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HACC-Based Nanoscale Delivery of the NbMLP28 Plasmid as a Crop Protection Strategy for Viral Diseases

[Image: see text] Resistant genes as an effective strategy to antivirus of plants are at the core of sustainability efforts. We use the antiviral protein major latex protein 28 (NbMLP28 plasmid) and N-2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) designated as the HACC/NbMLP28 complex...

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Autores principales: Zhang, Daoshun, Song, Liyun, Lin, Zhonglong, Huang, Kun, Liu, Chunming, Wang, Yong, Liu, Dongyang, Zhang, Songbai, Yang, Jinguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674983/
https://www.ncbi.nlm.nih.gov/pubmed/34926942
http://dx.doi.org/10.1021/acsomega.1c05295
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author Zhang, Daoshun
Song, Liyun
Lin, Zhonglong
Huang, Kun
Liu, Chunming
Wang, Yong
Liu, Dongyang
Zhang, Songbai
Yang, Jinguang
author_facet Zhang, Daoshun
Song, Liyun
Lin, Zhonglong
Huang, Kun
Liu, Chunming
Wang, Yong
Liu, Dongyang
Zhang, Songbai
Yang, Jinguang
author_sort Zhang, Daoshun
collection PubMed
description [Image: see text] Resistant genes as an effective strategy to antivirus of plants are at the core of sustainability efforts. We use the antiviral protein major latex protein 28 (NbMLP28 plasmid) and N-2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) designated as the HACC/NbMLP28 complex as protective gene delivery vectors to prepare nanonucleic acid drugs. The maximum drug loading capacity of HACC was 4. The particle size of HACC/NbMLP28 was measured by transmission electron microscopy and found to be approximately 40–120 nm, the particle dispersion index (PDI) was 0.448, and the ζ-potential was 22.3 mV. This facilitates its ability to deliver particles. Different controls of laser scanning confocal experiments verified the effective expression of NbMLP28 and the feasibility of nanodelivery. The optimal ratio of HACC/plasmid was 2:1. Finally, the nanoparticle/plasmid complex was tested for its ability to control diseases and was found to significantly improve resistance to three viruses. The enhanced resistance was particularly notable 4 days after inoculation. Taken together, these results indicate that HACC/NbMLP28 is a promising tool to treat plant viruses. To the best of our knowledge, this is the first study that successfully delivered and expressed antiviral protein particles in plants. This gene delivery system can effectively load antiviral plasmids and express them in plant leaves, significantly affecting the plant resistance of three RNA viruses.
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spelling pubmed-86749832021-12-17 HACC-Based Nanoscale Delivery of the NbMLP28 Plasmid as a Crop Protection Strategy for Viral Diseases Zhang, Daoshun Song, Liyun Lin, Zhonglong Huang, Kun Liu, Chunming Wang, Yong Liu, Dongyang Zhang, Songbai Yang, Jinguang ACS Omega [Image: see text] Resistant genes as an effective strategy to antivirus of plants are at the core of sustainability efforts. We use the antiviral protein major latex protein 28 (NbMLP28 plasmid) and N-2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) designated as the HACC/NbMLP28 complex as protective gene delivery vectors to prepare nanonucleic acid drugs. The maximum drug loading capacity of HACC was 4. The particle size of HACC/NbMLP28 was measured by transmission electron microscopy and found to be approximately 40–120 nm, the particle dispersion index (PDI) was 0.448, and the ζ-potential was 22.3 mV. This facilitates its ability to deliver particles. Different controls of laser scanning confocal experiments verified the effective expression of NbMLP28 and the feasibility of nanodelivery. The optimal ratio of HACC/plasmid was 2:1. Finally, the nanoparticle/plasmid complex was tested for its ability to control diseases and was found to significantly improve resistance to three viruses. The enhanced resistance was particularly notable 4 days after inoculation. Taken together, these results indicate that HACC/NbMLP28 is a promising tool to treat plant viruses. To the best of our knowledge, this is the first study that successfully delivered and expressed antiviral protein particles in plants. This gene delivery system can effectively load antiviral plasmids and express them in plant leaves, significantly affecting the plant resistance of three RNA viruses. American Chemical Society 2021-11-29 /pmc/articles/PMC8674983/ /pubmed/34926942 http://dx.doi.org/10.1021/acsomega.1c05295 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Zhang, Daoshun
Song, Liyun
Lin, Zhonglong
Huang, Kun
Liu, Chunming
Wang, Yong
Liu, Dongyang
Zhang, Songbai
Yang, Jinguang
HACC-Based Nanoscale Delivery of the NbMLP28 Plasmid as a Crop Protection Strategy for Viral Diseases
title HACC-Based Nanoscale Delivery of the NbMLP28 Plasmid as a Crop Protection Strategy for Viral Diseases
title_full HACC-Based Nanoscale Delivery of the NbMLP28 Plasmid as a Crop Protection Strategy for Viral Diseases
title_fullStr HACC-Based Nanoscale Delivery of the NbMLP28 Plasmid as a Crop Protection Strategy for Viral Diseases
title_full_unstemmed HACC-Based Nanoscale Delivery of the NbMLP28 Plasmid as a Crop Protection Strategy for Viral Diseases
title_short HACC-Based Nanoscale Delivery of the NbMLP28 Plasmid as a Crop Protection Strategy for Viral Diseases
title_sort hacc-based nanoscale delivery of the nbmlp28 plasmid as a crop protection strategy for viral diseases
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674983/
https://www.ncbi.nlm.nih.gov/pubmed/34926942
http://dx.doi.org/10.1021/acsomega.1c05295
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