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Associations between stool micro-transcriptome, gut microbiota, and infant growth

Rapid infant growth increases the risk for adult obesity. The gut microbiome is associated with early weight status; however, no study has examined how interactions between microbial and host ribonucleic acid (RNA) expression influence infant growth. We hypothesized that dynamics in infant stool mic...

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Autores principales: Carney, Molly C., Zhan, Xiang, Rangnekar, Akanksha, Chroneos, Maria Z., Craig, Sarah J.C., Makova, Kateryna D., Paul, Ian M., Hicks, Steven D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675179/
https://www.ncbi.nlm.nih.gov/pubmed/33407969
http://dx.doi.org/10.1017/S2040174420001324
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author Carney, Molly C.
Zhan, Xiang
Rangnekar, Akanksha
Chroneos, Maria Z.
Craig, Sarah J.C.
Makova, Kateryna D.
Paul, Ian M.
Hicks, Steven D.
author_facet Carney, Molly C.
Zhan, Xiang
Rangnekar, Akanksha
Chroneos, Maria Z.
Craig, Sarah J.C.
Makova, Kateryna D.
Paul, Ian M.
Hicks, Steven D.
author_sort Carney, Molly C.
collection PubMed
description Rapid infant growth increases the risk for adult obesity. The gut microbiome is associated with early weight status; however, no study has examined how interactions between microbial and host ribonucleic acid (RNA) expression influence infant growth. We hypothesized that dynamics in infant stool micro-ribonucleic acids (miRNAs) would be associated with both microbial activity and infant growth via putative metabolic targets. Stool was collected twice from 30 full-term infants, at 1 month and again between 6 and 12 months. Stool RNA were measured with high-throughput sequencing and aligned to human and microbial databases. Infant growth was measured by weight-for-length z-score at birth and 12 months. Increased RNA transcriptional activity of Clostridia (R = 0.55; Adj p = 3.7E-2) and Burkholderia (R = −0.820, Adj p = 2.62E-3) were associated with infant growth. Of the 25 human RNAs associated with growth, 16 were miRNAs. The miRNAs demonstrated significant target enrichment (Adj p < 0.05) for four metabolic pathways. There were four associations between growth-related miRNAs and growth-related phyla. We have shown that longitudinal trends in gut microbiota activity and human miRNA levels are associated with infant growth and the metabolic targets of miRNAs suggest these molecules may regulate the biosynthetic landscape of the gut and influence microbial activity.
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spelling pubmed-86751792022-12-01 Associations between stool micro-transcriptome, gut microbiota, and infant growth Carney, Molly C. Zhan, Xiang Rangnekar, Akanksha Chroneos, Maria Z. Craig, Sarah J.C. Makova, Kateryna D. Paul, Ian M. Hicks, Steven D. J Dev Orig Health Dis Article Rapid infant growth increases the risk for adult obesity. The gut microbiome is associated with early weight status; however, no study has examined how interactions between microbial and host ribonucleic acid (RNA) expression influence infant growth. We hypothesized that dynamics in infant stool micro-ribonucleic acids (miRNAs) would be associated with both microbial activity and infant growth via putative metabolic targets. Stool was collected twice from 30 full-term infants, at 1 month and again between 6 and 12 months. Stool RNA were measured with high-throughput sequencing and aligned to human and microbial databases. Infant growth was measured by weight-for-length z-score at birth and 12 months. Increased RNA transcriptional activity of Clostridia (R = 0.55; Adj p = 3.7E-2) and Burkholderia (R = −0.820, Adj p = 2.62E-3) were associated with infant growth. Of the 25 human RNAs associated with growth, 16 were miRNAs. The miRNAs demonstrated significant target enrichment (Adj p < 0.05) for four metabolic pathways. There were four associations between growth-related miRNAs and growth-related phyla. We have shown that longitudinal trends in gut microbiota activity and human miRNA levels are associated with infant growth and the metabolic targets of miRNAs suggest these molecules may regulate the biosynthetic landscape of the gut and influence microbial activity. 2021-12 2021-01-07 /pmc/articles/PMC8675179/ /pubmed/33407969 http://dx.doi.org/10.1017/S2040174420001324 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Carney, Molly C.
Zhan, Xiang
Rangnekar, Akanksha
Chroneos, Maria Z.
Craig, Sarah J.C.
Makova, Kateryna D.
Paul, Ian M.
Hicks, Steven D.
Associations between stool micro-transcriptome, gut microbiota, and infant growth
title Associations between stool micro-transcriptome, gut microbiota, and infant growth
title_full Associations between stool micro-transcriptome, gut microbiota, and infant growth
title_fullStr Associations between stool micro-transcriptome, gut microbiota, and infant growth
title_full_unstemmed Associations between stool micro-transcriptome, gut microbiota, and infant growth
title_short Associations between stool micro-transcriptome, gut microbiota, and infant growth
title_sort associations between stool micro-transcriptome, gut microbiota, and infant growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675179/
https://www.ncbi.nlm.nih.gov/pubmed/33407969
http://dx.doi.org/10.1017/S2040174420001324
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