Cargando…
Controlled human malaria infection with a clone of Plasmodium vivax with high-quality genome assembly
Controlled human malaria infection (CHMI) provides a highly informative means to investigate host-pathogen interactions and enable in vivo proof-of-concept efficacy testing of new drugs and vaccines. However, unlike Plasmodium falciparum, well-characterized P. vivax parasites that are safe and suita...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Clinical Investigation
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675201/ https://www.ncbi.nlm.nih.gov/pubmed/34609964 http://dx.doi.org/10.1172/jci.insight.152465 |
_version_ | 1784615833486491648 |
---|---|
author | Minassian, Angela M. Themistocleous, Yrene Silk, Sarah E. Barrett, Jordan R. Kemp, Alison Quinkert, Doris Nielsen, Carolyn M. Edwards, Nick J. Rawlinson, Thomas A. Ramos Lopez, Fernando Roobsoong, Wanlapa Ellis, Katherine J.D. Cho, Jee-Sun Aunin, Eerik Otto, Thomas D. Reid, Adam J. Bach, Florian A. Labbé, Geneviève M.C. Poulton, Ian D. Marini, Arianna Zaric, Marija Mulatier, Margaux Lopez Ramon, Raquel Baker, Megan Mitton, Celia H. Sousa, Jason C. Rachaphaew, Nattawan Kumpitak, Chalermpon Maneechai, Nongnuj Suansomjit, Chayanut Piteekan, Tianrat Hou, Mimi M. Khozoee, Baktash McHugh, Kirsty Roberts, David J. Lawrie, Alison M. Blagborough, Andrew M. Nugent, Fay L. Taylor, Iona J. Johnson, Kimberly J. Spence, Philip J. Sattabongkot, Jetsumon Biswas, Sumi Rayner, Julian C. Draper, Simon J. |
author_facet | Minassian, Angela M. Themistocleous, Yrene Silk, Sarah E. Barrett, Jordan R. Kemp, Alison Quinkert, Doris Nielsen, Carolyn M. Edwards, Nick J. Rawlinson, Thomas A. Ramos Lopez, Fernando Roobsoong, Wanlapa Ellis, Katherine J.D. Cho, Jee-Sun Aunin, Eerik Otto, Thomas D. Reid, Adam J. Bach, Florian A. Labbé, Geneviève M.C. Poulton, Ian D. Marini, Arianna Zaric, Marija Mulatier, Margaux Lopez Ramon, Raquel Baker, Megan Mitton, Celia H. Sousa, Jason C. Rachaphaew, Nattawan Kumpitak, Chalermpon Maneechai, Nongnuj Suansomjit, Chayanut Piteekan, Tianrat Hou, Mimi M. Khozoee, Baktash McHugh, Kirsty Roberts, David J. Lawrie, Alison M. Blagborough, Andrew M. Nugent, Fay L. Taylor, Iona J. Johnson, Kimberly J. Spence, Philip J. Sattabongkot, Jetsumon Biswas, Sumi Rayner, Julian C. Draper, Simon J. |
author_sort | Minassian, Angela M. |
collection | PubMed |
description | Controlled human malaria infection (CHMI) provides a highly informative means to investigate host-pathogen interactions and enable in vivo proof-of-concept efficacy testing of new drugs and vaccines. However, unlike Plasmodium falciparum, well-characterized P. vivax parasites that are safe and suitable for use in modern CHMI models are limited. Here, 2 healthy malaria-naive United Kingdom adults with universal donor blood group were safely infected with a clone of P. vivax from Thailand by mosquito-bite CHMI. Parasitemia developed in both volunteers, and prior to treatment, each volunteer donated blood to produce a cryopreserved stabilate of infected RBCs. Following stringent safety screening, the parasite stabilate from one of these donors (PvW1) was thawed and used to inoculate 6 healthy malaria-naive United Kingdom adults by blood-stage CHMI, at 3 different dilutions. Parasitemia developed in all volunteers, who were then successfully drug treated. PvW1 parasite DNA was isolated and sequenced to produce a high-quality genome assembly by using a hybrid assembly method. We analyzed leading vaccine candidate antigens and multigene families, including the vivax interspersed repeat (VIR) genes, of which we identified 1145 in the PvW1 genome. Our genomic analysis will guide future assessment of candidate vaccines and drugs, as well as experimental medicine studies. |
format | Online Article Text |
id | pubmed-8675201 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Clinical Investigation |
record_format | MEDLINE/PubMed |
spelling | pubmed-86752012021-12-21 Controlled human malaria infection with a clone of Plasmodium vivax with high-quality genome assembly Minassian, Angela M. Themistocleous, Yrene Silk, Sarah E. Barrett, Jordan R. Kemp, Alison Quinkert, Doris Nielsen, Carolyn M. Edwards, Nick J. Rawlinson, Thomas A. Ramos Lopez, Fernando Roobsoong, Wanlapa Ellis, Katherine J.D. Cho, Jee-Sun Aunin, Eerik Otto, Thomas D. Reid, Adam J. Bach, Florian A. Labbé, Geneviève M.C. Poulton, Ian D. Marini, Arianna Zaric, Marija Mulatier, Margaux Lopez Ramon, Raquel Baker, Megan Mitton, Celia H. Sousa, Jason C. Rachaphaew, Nattawan Kumpitak, Chalermpon Maneechai, Nongnuj Suansomjit, Chayanut Piteekan, Tianrat Hou, Mimi M. Khozoee, Baktash McHugh, Kirsty Roberts, David J. Lawrie, Alison M. Blagborough, Andrew M. Nugent, Fay L. Taylor, Iona J. Johnson, Kimberly J. Spence, Philip J. Sattabongkot, Jetsumon Biswas, Sumi Rayner, Julian C. Draper, Simon J. JCI Insight Resource and Technical Advance Controlled human malaria infection (CHMI) provides a highly informative means to investigate host-pathogen interactions and enable in vivo proof-of-concept efficacy testing of new drugs and vaccines. However, unlike Plasmodium falciparum, well-characterized P. vivax parasites that are safe and suitable for use in modern CHMI models are limited. Here, 2 healthy malaria-naive United Kingdom adults with universal donor blood group were safely infected with a clone of P. vivax from Thailand by mosquito-bite CHMI. Parasitemia developed in both volunteers, and prior to treatment, each volunteer donated blood to produce a cryopreserved stabilate of infected RBCs. Following stringent safety screening, the parasite stabilate from one of these donors (PvW1) was thawed and used to inoculate 6 healthy malaria-naive United Kingdom adults by blood-stage CHMI, at 3 different dilutions. Parasitemia developed in all volunteers, who were then successfully drug treated. PvW1 parasite DNA was isolated and sequenced to produce a high-quality genome assembly by using a hybrid assembly method. We analyzed leading vaccine candidate antigens and multigene families, including the vivax interspersed repeat (VIR) genes, of which we identified 1145 in the PvW1 genome. Our genomic analysis will guide future assessment of candidate vaccines and drugs, as well as experimental medicine studies. American Society for Clinical Investigation 2021-12-08 /pmc/articles/PMC8675201/ /pubmed/34609964 http://dx.doi.org/10.1172/jci.insight.152465 Text en © 2021 Minassian et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Resource and Technical Advance Minassian, Angela M. Themistocleous, Yrene Silk, Sarah E. Barrett, Jordan R. Kemp, Alison Quinkert, Doris Nielsen, Carolyn M. Edwards, Nick J. Rawlinson, Thomas A. Ramos Lopez, Fernando Roobsoong, Wanlapa Ellis, Katherine J.D. Cho, Jee-Sun Aunin, Eerik Otto, Thomas D. Reid, Adam J. Bach, Florian A. Labbé, Geneviève M.C. Poulton, Ian D. Marini, Arianna Zaric, Marija Mulatier, Margaux Lopez Ramon, Raquel Baker, Megan Mitton, Celia H. Sousa, Jason C. Rachaphaew, Nattawan Kumpitak, Chalermpon Maneechai, Nongnuj Suansomjit, Chayanut Piteekan, Tianrat Hou, Mimi M. Khozoee, Baktash McHugh, Kirsty Roberts, David J. Lawrie, Alison M. Blagborough, Andrew M. Nugent, Fay L. Taylor, Iona J. Johnson, Kimberly J. Spence, Philip J. Sattabongkot, Jetsumon Biswas, Sumi Rayner, Julian C. Draper, Simon J. Controlled human malaria infection with a clone of Plasmodium vivax with high-quality genome assembly |
title | Controlled human malaria infection with a clone of Plasmodium vivax with high-quality genome assembly |
title_full | Controlled human malaria infection with a clone of Plasmodium vivax with high-quality genome assembly |
title_fullStr | Controlled human malaria infection with a clone of Plasmodium vivax with high-quality genome assembly |
title_full_unstemmed | Controlled human malaria infection with a clone of Plasmodium vivax with high-quality genome assembly |
title_short | Controlled human malaria infection with a clone of Plasmodium vivax with high-quality genome assembly |
title_sort | controlled human malaria infection with a clone of plasmodium vivax with high-quality genome assembly |
topic | Resource and Technical Advance |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675201/ https://www.ncbi.nlm.nih.gov/pubmed/34609964 http://dx.doi.org/10.1172/jci.insight.152465 |
work_keys_str_mv | AT minassianangelam controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT themistocleousyrene controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT silksarahe controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT barrettjordanr controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT kempalison controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT quinkertdoris controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT nielsencarolynm controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT edwardsnickj controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT rawlinsonthomasa controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT ramoslopezfernando controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT roobsoongwanlapa controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT elliskatherinejd controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT chojeesun controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT aunineerik controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT ottothomasd controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT reidadamj controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT bachfloriana controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT labbegenevievemc controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT poultoniand controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT mariniarianna controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT zaricmarija controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT mulatiermargaux controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT lopezramonraquel controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT bakermegan controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT mittonceliah controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT sousajasonc controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT rachaphaewnattawan controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT kumpitakchalermpon controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT maneechainongnuj controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT suansomjitchayanut controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT piteekantianrat controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT houmimim controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT khozoeebaktash controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT mchughkirsty controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT robertsdavidj controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT lawriealisonm controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT blagboroughandrewm controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT nugentfayl controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT taylorionaj controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT johnsonkimberlyj controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT spencephilipj controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT sattabongkotjetsumon controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT biswassumi controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT raynerjulianc controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly AT drapersimonj controlledhumanmalariainfectionwithacloneofplasmodiumvivaxwithhighqualitygenomeassembly |