Cargando…
Metabolic Profiling and Gene Expression Analysis Unveil Differences in Flavonoid and Lipid Metabolisms Between ‘Huapi’ Kumquat (Fortunella crassifolia Swingle) and Its Wild Type
To elucidate the mechanism underlying special characteristic differences between a spontaneous seedling mutant ‘Huapi’ kumquat (HP) and its wild-type ‘Rongan’ kumquat (RA), the fruit quality, metabolic profiles, and gene expressions of the peel and flesh were comprehensively analyzed. Compared with...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675212/ https://www.ncbi.nlm.nih.gov/pubmed/34925410 http://dx.doi.org/10.3389/fpls.2021.759968 |
_version_ | 1784615835615100928 |
---|---|
author | Ma, Qiaoli Hu, Yongwei Dong, Xinghua Zhou, Gaofeng Liu, Xiao Gu, Qingqing Wei, Qingjiang |
author_facet | Ma, Qiaoli Hu, Yongwei Dong, Xinghua Zhou, Gaofeng Liu, Xiao Gu, Qingqing Wei, Qingjiang |
author_sort | Ma, Qiaoli |
collection | PubMed |
description | To elucidate the mechanism underlying special characteristic differences between a spontaneous seedling mutant ‘Huapi’ kumquat (HP) and its wild-type ‘Rongan’ kumquat (RA), the fruit quality, metabolic profiles, and gene expressions of the peel and flesh were comprehensively analyzed. Compared with RA, HP fruit has distinctive phenotypes such as glossy peel, light color, and few amounts of oil glands. Interestingly, HP also accumulated higher flavonoid (approximately 4.1-fold changes) than RA. Based on metabolomics analysis, we identified 201 differential compounds, including 65 flavonoids and 37 lipids. Most of the differential flavonoids were glycosylated by hexoside and accumulated higher contents in the peel but lower in the flesh of HP than those of RA fruit. For differential lipids, most of them belonged to lysophosphatidycholines (LysoPCs) and lysophosphatidylethanolamines (LysoPEs) and exhibited low abundance in both peel and flesh of HP fruit. In addition, structural genes associated with the flavonoid and lipid pathways were differentially regulated between the two kumquat varieties. Gene expression analysis also revealed the significant roles of UDP-glycosyltransferase (UGT) and phospholipase genes in flavonoid and glycerophospholipid metabolisms, respectively. These findings provide valuable information for interpreting the mutation mechanism of HP kumquat. |
format | Online Article Text |
id | pubmed-8675212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86752122021-12-17 Metabolic Profiling and Gene Expression Analysis Unveil Differences in Flavonoid and Lipid Metabolisms Between ‘Huapi’ Kumquat (Fortunella crassifolia Swingle) and Its Wild Type Ma, Qiaoli Hu, Yongwei Dong, Xinghua Zhou, Gaofeng Liu, Xiao Gu, Qingqing Wei, Qingjiang Front Plant Sci Plant Science To elucidate the mechanism underlying special characteristic differences between a spontaneous seedling mutant ‘Huapi’ kumquat (HP) and its wild-type ‘Rongan’ kumquat (RA), the fruit quality, metabolic profiles, and gene expressions of the peel and flesh were comprehensively analyzed. Compared with RA, HP fruit has distinctive phenotypes such as glossy peel, light color, and few amounts of oil glands. Interestingly, HP also accumulated higher flavonoid (approximately 4.1-fold changes) than RA. Based on metabolomics analysis, we identified 201 differential compounds, including 65 flavonoids and 37 lipids. Most of the differential flavonoids were glycosylated by hexoside and accumulated higher contents in the peel but lower in the flesh of HP than those of RA fruit. For differential lipids, most of them belonged to lysophosphatidycholines (LysoPCs) and lysophosphatidylethanolamines (LysoPEs) and exhibited low abundance in both peel and flesh of HP fruit. In addition, structural genes associated with the flavonoid and lipid pathways were differentially regulated between the two kumquat varieties. Gene expression analysis also revealed the significant roles of UDP-glycosyltransferase (UGT) and phospholipase genes in flavonoid and glycerophospholipid metabolisms, respectively. These findings provide valuable information for interpreting the mutation mechanism of HP kumquat. Frontiers Media S.A. 2021-12-02 /pmc/articles/PMC8675212/ /pubmed/34925410 http://dx.doi.org/10.3389/fpls.2021.759968 Text en Copyright © 2021 Ma, Hu, Dong, Zhou, Liu, Gu and Wei. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Ma, Qiaoli Hu, Yongwei Dong, Xinghua Zhou, Gaofeng Liu, Xiao Gu, Qingqing Wei, Qingjiang Metabolic Profiling and Gene Expression Analysis Unveil Differences in Flavonoid and Lipid Metabolisms Between ‘Huapi’ Kumquat (Fortunella crassifolia Swingle) and Its Wild Type |
title | Metabolic Profiling and Gene Expression Analysis Unveil Differences in Flavonoid and Lipid Metabolisms Between ‘Huapi’ Kumquat (Fortunella crassifolia Swingle) and Its Wild Type |
title_full | Metabolic Profiling and Gene Expression Analysis Unveil Differences in Flavonoid and Lipid Metabolisms Between ‘Huapi’ Kumquat (Fortunella crassifolia Swingle) and Its Wild Type |
title_fullStr | Metabolic Profiling and Gene Expression Analysis Unveil Differences in Flavonoid and Lipid Metabolisms Between ‘Huapi’ Kumquat (Fortunella crassifolia Swingle) and Its Wild Type |
title_full_unstemmed | Metabolic Profiling and Gene Expression Analysis Unveil Differences in Flavonoid and Lipid Metabolisms Between ‘Huapi’ Kumquat (Fortunella crassifolia Swingle) and Its Wild Type |
title_short | Metabolic Profiling and Gene Expression Analysis Unveil Differences in Flavonoid and Lipid Metabolisms Between ‘Huapi’ Kumquat (Fortunella crassifolia Swingle) and Its Wild Type |
title_sort | metabolic profiling and gene expression analysis unveil differences in flavonoid and lipid metabolisms between ‘huapi’ kumquat (fortunella crassifolia swingle) and its wild type |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675212/ https://www.ncbi.nlm.nih.gov/pubmed/34925410 http://dx.doi.org/10.3389/fpls.2021.759968 |
work_keys_str_mv | AT maqiaoli metabolicprofilingandgeneexpressionanalysisunveildifferencesinflavonoidandlipidmetabolismsbetweenhuapikumquatfortunellacrassifoliaswingleanditswildtype AT huyongwei metabolicprofilingandgeneexpressionanalysisunveildifferencesinflavonoidandlipidmetabolismsbetweenhuapikumquatfortunellacrassifoliaswingleanditswildtype AT dongxinghua metabolicprofilingandgeneexpressionanalysisunveildifferencesinflavonoidandlipidmetabolismsbetweenhuapikumquatfortunellacrassifoliaswingleanditswildtype AT zhougaofeng metabolicprofilingandgeneexpressionanalysisunveildifferencesinflavonoidandlipidmetabolismsbetweenhuapikumquatfortunellacrassifoliaswingleanditswildtype AT liuxiao metabolicprofilingandgeneexpressionanalysisunveildifferencesinflavonoidandlipidmetabolismsbetweenhuapikumquatfortunellacrassifoliaswingleanditswildtype AT guqingqing metabolicprofilingandgeneexpressionanalysisunveildifferencesinflavonoidandlipidmetabolismsbetweenhuapikumquatfortunellacrassifoliaswingleanditswildtype AT weiqingjiang metabolicprofilingandgeneexpressionanalysisunveildifferencesinflavonoidandlipidmetabolismsbetweenhuapikumquatfortunellacrassifoliaswingleanditswildtype |