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Design considerations for analyzing protein translation regulation by condensates
One proposed role for biomolecular condensates that contain RNA is translation regulation. In several specific contexts, translation has been shown to be modulated by the presence of a phase-separating protein and under conditions which promote phase separation, and likely many more await discovery....
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675288/ https://www.ncbi.nlm.nih.gov/pubmed/34670845 http://dx.doi.org/10.1261/rna.079002.121 |
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author | Roden, Christine A. Gladfelter, Amy S. |
author_facet | Roden, Christine A. Gladfelter, Amy S. |
author_sort | Roden, Christine A. |
collection | PubMed |
description | One proposed role for biomolecular condensates that contain RNA is translation regulation. In several specific contexts, translation has been shown to be modulated by the presence of a phase-separating protein and under conditions which promote phase separation, and likely many more await discovery. A powerful tool for determining the rules for condensate-dependent translation is the use of engineered RNA sequences, which can serve as reporters for translation efficiency. This Perspective will discuss design features to consider in engineering RNA reporters to determine the role of phase separation in translational regulation. Specifically, we will cover (i) how to engineer RNA sequence to recapitulate native protein/RNA interactions, (ii) the advantages and disadvantages for commonly used reporter RNA sequences, and (iii) important control experiments to distinguish between binding- and condensation-dependent translational repression. The goal of this review is to promote the design and application of faithful translation reporters to demonstrate a physiological role of biomolecular condensates in translation. |
format | Online Article Text |
id | pubmed-8675288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-86752882022-01-01 Design considerations for analyzing protein translation regulation by condensates Roden, Christine A. Gladfelter, Amy S. RNA Perspective One proposed role for biomolecular condensates that contain RNA is translation regulation. In several specific contexts, translation has been shown to be modulated by the presence of a phase-separating protein and under conditions which promote phase separation, and likely many more await discovery. A powerful tool for determining the rules for condensate-dependent translation is the use of engineered RNA sequences, which can serve as reporters for translation efficiency. This Perspective will discuss design features to consider in engineering RNA reporters to determine the role of phase separation in translational regulation. Specifically, we will cover (i) how to engineer RNA sequence to recapitulate native protein/RNA interactions, (ii) the advantages and disadvantages for commonly used reporter RNA sequences, and (iii) important control experiments to distinguish between binding- and condensation-dependent translational repression. The goal of this review is to promote the design and application of faithful translation reporters to demonstrate a physiological role of biomolecular condensates in translation. Cold Spring Harbor Laboratory Press 2022-01 /pmc/articles/PMC8675288/ /pubmed/34670845 http://dx.doi.org/10.1261/rna.079002.121 Text en © 2022 Roden and Gladfelter; Published by Cold Spring Harbor Laboratory Press for the RNA Society https://creativecommons.org/licenses/by-nc/4.0/This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Perspective Roden, Christine A. Gladfelter, Amy S. Design considerations for analyzing protein translation regulation by condensates |
title | Design considerations for analyzing protein translation regulation by condensates |
title_full | Design considerations for analyzing protein translation regulation by condensates |
title_fullStr | Design considerations for analyzing protein translation regulation by condensates |
title_full_unstemmed | Design considerations for analyzing protein translation regulation by condensates |
title_short | Design considerations for analyzing protein translation regulation by condensates |
title_sort | design considerations for analyzing protein translation regulation by condensates |
topic | Perspective |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675288/ https://www.ncbi.nlm.nih.gov/pubmed/34670845 http://dx.doi.org/10.1261/rna.079002.121 |
work_keys_str_mv | AT rodenchristinea designconsiderationsforanalyzingproteintranslationregulationbycondensates AT gladfelteramys designconsiderationsforanalyzingproteintranslationregulationbycondensates |