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Design considerations for analyzing protein translation regulation by condensates

One proposed role for biomolecular condensates that contain RNA is translation regulation. In several specific contexts, translation has been shown to be modulated by the presence of a phase-separating protein and under conditions which promote phase separation, and likely many more await discovery....

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Detalles Bibliográficos
Autores principales: Roden, Christine A., Gladfelter, Amy S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675288/
https://www.ncbi.nlm.nih.gov/pubmed/34670845
http://dx.doi.org/10.1261/rna.079002.121
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author Roden, Christine A.
Gladfelter, Amy S.
author_facet Roden, Christine A.
Gladfelter, Amy S.
author_sort Roden, Christine A.
collection PubMed
description One proposed role for biomolecular condensates that contain RNA is translation regulation. In several specific contexts, translation has been shown to be modulated by the presence of a phase-separating protein and under conditions which promote phase separation, and likely many more await discovery. A powerful tool for determining the rules for condensate-dependent translation is the use of engineered RNA sequences, which can serve as reporters for translation efficiency. This Perspective will discuss design features to consider in engineering RNA reporters to determine the role of phase separation in translational regulation. Specifically, we will cover (i) how to engineer RNA sequence to recapitulate native protein/RNA interactions, (ii) the advantages and disadvantages for commonly used reporter RNA sequences, and (iii) important control experiments to distinguish between binding- and condensation-dependent translational repression. The goal of this review is to promote the design and application of faithful translation reporters to demonstrate a physiological role of biomolecular condensates in translation.
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spelling pubmed-86752882022-01-01 Design considerations for analyzing protein translation regulation by condensates Roden, Christine A. Gladfelter, Amy S. RNA Perspective One proposed role for biomolecular condensates that contain RNA is translation regulation. In several specific contexts, translation has been shown to be modulated by the presence of a phase-separating protein and under conditions which promote phase separation, and likely many more await discovery. A powerful tool for determining the rules for condensate-dependent translation is the use of engineered RNA sequences, which can serve as reporters for translation efficiency. This Perspective will discuss design features to consider in engineering RNA reporters to determine the role of phase separation in translational regulation. Specifically, we will cover (i) how to engineer RNA sequence to recapitulate native protein/RNA interactions, (ii) the advantages and disadvantages for commonly used reporter RNA sequences, and (iii) important control experiments to distinguish between binding- and condensation-dependent translational repression. The goal of this review is to promote the design and application of faithful translation reporters to demonstrate a physiological role of biomolecular condensates in translation. Cold Spring Harbor Laboratory Press 2022-01 /pmc/articles/PMC8675288/ /pubmed/34670845 http://dx.doi.org/10.1261/rna.079002.121 Text en © 2022 Roden and Gladfelter; Published by Cold Spring Harbor Laboratory Press for the RNA Society https://creativecommons.org/licenses/by-nc/4.0/This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Perspective
Roden, Christine A.
Gladfelter, Amy S.
Design considerations for analyzing protein translation regulation by condensates
title Design considerations for analyzing protein translation regulation by condensates
title_full Design considerations for analyzing protein translation regulation by condensates
title_fullStr Design considerations for analyzing protein translation regulation by condensates
title_full_unstemmed Design considerations for analyzing protein translation regulation by condensates
title_short Design considerations for analyzing protein translation regulation by condensates
title_sort design considerations for analyzing protein translation regulation by condensates
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675288/
https://www.ncbi.nlm.nih.gov/pubmed/34670845
http://dx.doi.org/10.1261/rna.079002.121
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