Coordinated Actions of Cas9 HNH and RuvC Nuclease Domains Are Regulated by the Bridge Helix and the Target DNA Sequence

[Image: see text] CRISPR-Cas systems are RNA-guided nucleases that provide adaptive immune protection in bacteria and archaea against intruding genomic materials. Cas9, a type-II CRISPR effector protein, is widely used for gene editing applications since a single guide RNA can direct Cas9 to cleave...

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Detalles Bibliográficos
Autores principales: Babu, Kesavan, Kathiresan, Venkatesan, Kumari, Pratibha, Newsom, Sydney, Parameshwaran, Hari Priya, Chen, Xiongping, Liu, Jin, Qin, Peter Z., Rajan, Rakhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675354/
https://www.ncbi.nlm.nih.gov/pubmed/34757726
http://dx.doi.org/10.1021/acs.biochem.1c00354

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