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Improved methanol tolerance of Rhizomucor miehei lipase based on N‑glycosylation within the α-helix region and its application in biodiesel production

BACKGROUND: Liquid lipases are widely used to convert oil into biodiesel. Methanol-resistant lipases with high catalytic activity are the first choice for practical production. Rhizomucor miehei lipase (RML) is a single-chain α/β-type protein that is widely used in biodiesel preparation. Improving t...

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Detalles Bibliográficos
Autores principales: Tian, Miao, Yang, Lingmei, Wang, Zhiyuan, Lv, Pengmei, Fu, Junying, Miao, Changlin, Li, Ming, Liu, Tao, Luo, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675521/
https://www.ncbi.nlm.nih.gov/pubmed/34911574
http://dx.doi.org/10.1186/s13068-021-02087-6