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Improved methanol tolerance of Rhizomucor miehei lipase based on N‑glycosylation within the α-helix region and its application in biodiesel production
BACKGROUND: Liquid lipases are widely used to convert oil into biodiesel. Methanol-resistant lipases with high catalytic activity are the first choice for practical production. Rhizomucor miehei lipase (RML) is a single-chain α/β-type protein that is widely used in biodiesel preparation. Improving t...
Autores principales: | Tian, Miao, Yang, Lingmei, Wang, Zhiyuan, Lv, Pengmei, Fu, Junying, Miao, Changlin, Li, Ming, Liu, Tao, Luo, Wen |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675521/ https://www.ncbi.nlm.nih.gov/pubmed/34911574 http://dx.doi.org/10.1186/s13068-021-02087-6 |
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