Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect

The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature...

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Detalles Bibliográficos
Autores principales: Vulders, R. C. M., van Hoogenhuizen, R. C., van der Giessen, E., van der Zaag, P. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675714/
https://www.ncbi.nlm.nih.gov/pubmed/34914768
http://dx.doi.org/10.1371/journal.pone.0261417
Descripción
Sumario:The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature are incoherent. Here, we report that for a classical clearing agent, benzyl-alcohol benzyl-benzoate (BABB), the shrinkage decreases significantly with increasing sample size, and present an analytical formula describing this.

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