Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect

The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature...

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Autores principales: Vulders, R. C. M., van Hoogenhuizen, R. C., van der Giessen, E., van der Zaag, P. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675714/
https://www.ncbi.nlm.nih.gov/pubmed/34914768
http://dx.doi.org/10.1371/journal.pone.0261417
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author Vulders, R. C. M.
van Hoogenhuizen, R. C.
van der Giessen, E.
van der Zaag, P. J.
author_facet Vulders, R. C. M.
van Hoogenhuizen, R. C.
van der Giessen, E.
van der Zaag, P. J.
author_sort Vulders, R. C. M.
collection PubMed
description The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature are incoherent. Here, we report that for a classical clearing agent, benzyl-alcohol benzyl-benzoate (BABB), the shrinkage decreases significantly with increasing sample size, and present an analytical formula describing this.
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spelling pubmed-86757142021-12-17 Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect Vulders, R. C. M. van Hoogenhuizen, R. C. van der Giessen, E. van der Zaag, P. J. PLoS One Research Article The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature are incoherent. Here, we report that for a classical clearing agent, benzyl-alcohol benzyl-benzoate (BABB), the shrinkage decreases significantly with increasing sample size, and present an analytical formula describing this. Public Library of Science 2021-12-16 /pmc/articles/PMC8675714/ /pubmed/34914768 http://dx.doi.org/10.1371/journal.pone.0261417 Text en © 2021 Vulders et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Vulders, R. C. M.
van Hoogenhuizen, R. C.
van der Giessen, E.
van der Zaag, P. J.
Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect
title Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect
title_full Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect
title_fullStr Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect
title_full_unstemmed Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect
title_short Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect
title_sort clearing-induced tisssue shrinkage: a novel observation of a thickness size effect
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675714/
https://www.ncbi.nlm.nih.gov/pubmed/34914768
http://dx.doi.org/10.1371/journal.pone.0261417
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