Cargando…

The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein

There are a wide variety of porcine parvoviruses (PPVs) referred to as PPV1 to PPV7. The latter was discovered in 2016 and later reported in some countries in America, Asia, and Europe. PPV7 as a pathogenic agent or coinfection with other pathogens causing disease has not yet been determined. In the...

Descripción completa

Detalles Bibliográficos
Autores principales: Vargas-Bermudez, Diana S., Rendon-Marin, Santiago, Ruiz-Saenz, Julian, Mogollón, Dario, Jaime, Jairo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675767/
https://www.ncbi.nlm.nih.gov/pubmed/34914702
http://dx.doi.org/10.1371/journal.pone.0258311
_version_ 1784615941637668864
author Vargas-Bermudez, Diana S.
Rendon-Marin, Santiago
Ruiz-Saenz, Julian
Mogollón, Dario
Jaime, Jairo
author_facet Vargas-Bermudez, Diana S.
Rendon-Marin, Santiago
Ruiz-Saenz, Julian
Mogollón, Dario
Jaime, Jairo
author_sort Vargas-Bermudez, Diana S.
collection PubMed
description There are a wide variety of porcine parvoviruses (PPVs) referred to as PPV1 to PPV7. The latter was discovered in 2016 and later reported in some countries in America, Asia, and Europe. PPV7 as a pathogenic agent or coinfection with other pathogens causing disease has not yet been determined. In the present study, we report the identification of PPV7 for the first time in Colombia, where it was found retrospectively since 2015 in 40% of the provinces that make up the country (13/32), and the virus was ratified for 2018 in 4/5 provinces evaluated. Additionally, partial sequencing (nucleotides 380 to 4000) was performed of four Colombian strains completely covering the VP2 and NS1 viral genes. A sequence identity greater than 99% was found when comparing them with reference strains from the USA and China. In three of the four Colombian strains, an insertion of 15 nucleotides (five amino acids) was found in the PPV7-VP2 capsid protein (540–5554 nt; 180–184 aa). Based on this insertion, the VP2 phylogenetic analysis exhibited two well-differentiated evolutionarily related groups. To evaluate the impact of this insertion on the structure of the PPV7-VP2 capsid protein, the secondary structure of two different Colombian strains was predicted, and it was determined that the insertion is located in the coil region and not involved in significant changes in the structure of the protein. The 3D structure of the PPV7-VP2 capsid protein was determined by threading and homology modeling, and it was shown that the insertion did not imply a change in the shape of the protein. Additionally, it was determined that the insertion is not involved in suppressing a potential B cell epitope, although the increase in length of the epitope could affect the interaction with molecules that allow a specific immune response.
format Online
Article
Text
id pubmed-8675767
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-86757672021-12-17 The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein Vargas-Bermudez, Diana S. Rendon-Marin, Santiago Ruiz-Saenz, Julian Mogollón, Dario Jaime, Jairo PLoS One Research Article There are a wide variety of porcine parvoviruses (PPVs) referred to as PPV1 to PPV7. The latter was discovered in 2016 and later reported in some countries in America, Asia, and Europe. PPV7 as a pathogenic agent or coinfection with other pathogens causing disease has not yet been determined. In the present study, we report the identification of PPV7 for the first time in Colombia, where it was found retrospectively since 2015 in 40% of the provinces that make up the country (13/32), and the virus was ratified for 2018 in 4/5 provinces evaluated. Additionally, partial sequencing (nucleotides 380 to 4000) was performed of four Colombian strains completely covering the VP2 and NS1 viral genes. A sequence identity greater than 99% was found when comparing them with reference strains from the USA and China. In three of the four Colombian strains, an insertion of 15 nucleotides (five amino acids) was found in the PPV7-VP2 capsid protein (540–5554 nt; 180–184 aa). Based on this insertion, the VP2 phylogenetic analysis exhibited two well-differentiated evolutionarily related groups. To evaluate the impact of this insertion on the structure of the PPV7-VP2 capsid protein, the secondary structure of two different Colombian strains was predicted, and it was determined that the insertion is located in the coil region and not involved in significant changes in the structure of the protein. The 3D structure of the PPV7-VP2 capsid protein was determined by threading and homology modeling, and it was shown that the insertion did not imply a change in the shape of the protein. Additionally, it was determined that the insertion is not involved in suppressing a potential B cell epitope, although the increase in length of the epitope could affect the interaction with molecules that allow a specific immune response. Public Library of Science 2021-12-16 /pmc/articles/PMC8675767/ /pubmed/34914702 http://dx.doi.org/10.1371/journal.pone.0258311 Text en © 2021 Vargas-Bermudez et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Vargas-Bermudez, Diana S.
Rendon-Marin, Santiago
Ruiz-Saenz, Julian
Mogollón, Dario
Jaime, Jairo
The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein
title The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein
title_full The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein
title_fullStr The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein
title_full_unstemmed The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein
title_short The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein
title_sort first report of porcine parvovirus 7 (ppv7) in colombia demonstrates the presence of variants associated with modifications at the level of the vp2-capsid protein
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675767/
https://www.ncbi.nlm.nih.gov/pubmed/34914702
http://dx.doi.org/10.1371/journal.pone.0258311
work_keys_str_mv AT vargasbermudezdianas thefirstreportofporcineparvovirus7ppv7incolombiademonstratesthepresenceofvariantsassociatedwithmodificationsatthelevelofthevp2capsidprotein
AT rendonmarinsantiago thefirstreportofporcineparvovirus7ppv7incolombiademonstratesthepresenceofvariantsassociatedwithmodificationsatthelevelofthevp2capsidprotein
AT ruizsaenzjulian thefirstreportofporcineparvovirus7ppv7incolombiademonstratesthepresenceofvariantsassociatedwithmodificationsatthelevelofthevp2capsidprotein
AT mogollondario thefirstreportofporcineparvovirus7ppv7incolombiademonstratesthepresenceofvariantsassociatedwithmodificationsatthelevelofthevp2capsidprotein
AT jaimejairo thefirstreportofporcineparvovirus7ppv7incolombiademonstratesthepresenceofvariantsassociatedwithmodificationsatthelevelofthevp2capsidprotein
AT vargasbermudezdianas firstreportofporcineparvovirus7ppv7incolombiademonstratesthepresenceofvariantsassociatedwithmodificationsatthelevelofthevp2capsidprotein
AT rendonmarinsantiago firstreportofporcineparvovirus7ppv7incolombiademonstratesthepresenceofvariantsassociatedwithmodificationsatthelevelofthevp2capsidprotein
AT ruizsaenzjulian firstreportofporcineparvovirus7ppv7incolombiademonstratesthepresenceofvariantsassociatedwithmodificationsatthelevelofthevp2capsidprotein
AT mogollondario firstreportofporcineparvovirus7ppv7incolombiademonstratesthepresenceofvariantsassociatedwithmodificationsatthelevelofthevp2capsidprotein
AT jaimejairo firstreportofporcineparvovirus7ppv7incolombiademonstratesthepresenceofvariantsassociatedwithmodificationsatthelevelofthevp2capsidprotein