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Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites
Artemisinin-based combination therapies (ACT) are the frontline treatments against malaria worldwide. Recently the use of traditional infusions from Artemisia annua (from which artemisinin is obtained) or Artemisia afra (lacking artemisinin) has been controversially advocated. Such unregulated plant...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675911/ https://www.ncbi.nlm.nih.gov/pubmed/34857648 http://dx.doi.org/10.26508/lsa.202101237 |
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author | Ashraf, Kutub Tajeri, Shahin Arnold, Christophe-Sébastien Amanzougaghene, Nadia Franetich, Jean-François Vantaux, Amélie Soulard, Valérie Bordessoulles, Mallaury Cazals, Guillaume Bousema, Teun van Gemert, Geert-Jan Le Grand, Roger Dereuddre-Bosquet, Nathalie Barale, Jean-Christophe Witkowski, Benoit Snounou, Georges Duval, Romain Botté, Cyrille Y Mazier, Dominique |
author_facet | Ashraf, Kutub Tajeri, Shahin Arnold, Christophe-Sébastien Amanzougaghene, Nadia Franetich, Jean-François Vantaux, Amélie Soulard, Valérie Bordessoulles, Mallaury Cazals, Guillaume Bousema, Teun van Gemert, Geert-Jan Le Grand, Roger Dereuddre-Bosquet, Nathalie Barale, Jean-Christophe Witkowski, Benoit Snounou, Georges Duval, Romain Botté, Cyrille Y Mazier, Dominique |
author_sort | Ashraf, Kutub |
collection | PubMed |
description | Artemisinin-based combination therapies (ACT) are the frontline treatments against malaria worldwide. Recently the use of traditional infusions from Artemisia annua (from which artemisinin is obtained) or Artemisia afra (lacking artemisinin) has been controversially advocated. Such unregulated plant-based remedies are strongly discouraged as they might constitute sub-optimal therapies and promote drug resistance. Here, we conducted the first comparative study of the anti-malarial effects of both plant infusions in vitro against the asexual erythrocytic stages of Plasmodium falciparum and the pre-erythrocytic (i.e., liver) stages of various Plasmodium species. Low concentrations of either infusion accounted for significant inhibitory activities across every parasite species and stage studied. We show that these antiplasmodial effects were essentially artemisinin-independent and were additionally monitored by observations of the parasite apicoplast and mitochondrion. In particular, the infusions significantly incapacitated sporozoites, and for Plasmodium vivax and P. cynomolgi, disrupted the hypnozoites. This provides the first indication that compounds other than 8-aminoquinolines could be effective antimalarials against relapsing parasites. These observations advocate for further screening to uncover urgently needed novel antimalarial lead compounds. |
format | Online Article Text |
id | pubmed-8675911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-86759112022-01-05 Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites Ashraf, Kutub Tajeri, Shahin Arnold, Christophe-Sébastien Amanzougaghene, Nadia Franetich, Jean-François Vantaux, Amélie Soulard, Valérie Bordessoulles, Mallaury Cazals, Guillaume Bousema, Teun van Gemert, Geert-Jan Le Grand, Roger Dereuddre-Bosquet, Nathalie Barale, Jean-Christophe Witkowski, Benoit Snounou, Georges Duval, Romain Botté, Cyrille Y Mazier, Dominique Life Sci Alliance Research Articles Artemisinin-based combination therapies (ACT) are the frontline treatments against malaria worldwide. Recently the use of traditional infusions from Artemisia annua (from which artemisinin is obtained) or Artemisia afra (lacking artemisinin) has been controversially advocated. Such unregulated plant-based remedies are strongly discouraged as they might constitute sub-optimal therapies and promote drug resistance. Here, we conducted the first comparative study of the anti-malarial effects of both plant infusions in vitro against the asexual erythrocytic stages of Plasmodium falciparum and the pre-erythrocytic (i.e., liver) stages of various Plasmodium species. Low concentrations of either infusion accounted for significant inhibitory activities across every parasite species and stage studied. We show that these antiplasmodial effects were essentially artemisinin-independent and were additionally monitored by observations of the parasite apicoplast and mitochondrion. In particular, the infusions significantly incapacitated sporozoites, and for Plasmodium vivax and P. cynomolgi, disrupted the hypnozoites. This provides the first indication that compounds other than 8-aminoquinolines could be effective antimalarials against relapsing parasites. These observations advocate for further screening to uncover urgently needed novel antimalarial lead compounds. Life Science Alliance LLC 2021-12-02 /pmc/articles/PMC8675911/ /pubmed/34857648 http://dx.doi.org/10.26508/lsa.202101237 Text en © 2021 Ashraf et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Ashraf, Kutub Tajeri, Shahin Arnold, Christophe-Sébastien Amanzougaghene, Nadia Franetich, Jean-François Vantaux, Amélie Soulard, Valérie Bordessoulles, Mallaury Cazals, Guillaume Bousema, Teun van Gemert, Geert-Jan Le Grand, Roger Dereuddre-Bosquet, Nathalie Barale, Jean-Christophe Witkowski, Benoit Snounou, Georges Duval, Romain Botté, Cyrille Y Mazier, Dominique Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites |
title | Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites |
title_full | Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites |
title_fullStr | Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites |
title_full_unstemmed | Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites |
title_short | Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites |
title_sort | artemisinin-independent inhibitory activity of artemisia sp. infusions against different plasmodium stages including relapse-causing hypnozoites |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675911/ https://www.ncbi.nlm.nih.gov/pubmed/34857648 http://dx.doi.org/10.26508/lsa.202101237 |
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