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Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications

Nanofibers as elements for bioscaffolds are pushing the development of tissue engineering. In this study, tussah silk was mechanically disintegrated into nanofibers dispersed in aqueous solution which was cast to generate tussah silk fibroin (TSF) nanofiber mats. The effect of treatment time on the...

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Autores principales: Chen, Ming, Qin, Jianzhong, Lu, Shijun, Zhang, Feng, Zuo, Baoqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8677428/
https://www.ncbi.nlm.nih.gov/pubmed/34926415
http://dx.doi.org/10.3389/fbioe.2021.746016
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author Chen, Ming
Qin, Jianzhong
Lu, Shijun
Zhang, Feng
Zuo, Baoqi
author_facet Chen, Ming
Qin, Jianzhong
Lu, Shijun
Zhang, Feng
Zuo, Baoqi
author_sort Chen, Ming
collection PubMed
description Nanofibers as elements for bioscaffolds are pushing the development of tissue engineering. In this study, tussah silk was mechanically disintegrated into nanofibers dispersed in aqueous solution which was cast to generate tussah silk fibroin (TSF) nanofiber mats. The effect of treatment time on the morphology, structure, and mechanical properties of nanofiber mats was examined. SEM indicated decreasing diameter of the nanofiber with shearing time, and the diameter of the nanofiber was 139.7 nm after 30 min treatment. These nanofiber mats exhibited excellent mechanical properties; the breaking strength increased from 26.31 to 72.68 MPa with the decrease of fiber diameter from 196.5 to 139.7 nm. The particulate debris was observed on protease XIV degraded nanofiber mats, and the weight loss was greater than 10% after 30 days in vitro degradation. The cell compatibility experiment confirmed adhesion and spreading of NIH-3T3 cells and enhanced cell proliferation on TSF nanofiber mats compared to that on Bombyx mori silk nanofiber mats. In conclusion, results indicate that TSF nanofiber mats prepared in this study are mechanically robust, slow biodegradable, and biocompatible materials, and have promising application in regenerative medicine.
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spelling pubmed-86774282021-12-17 Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications Chen, Ming Qin, Jianzhong Lu, Shijun Zhang, Feng Zuo, Baoqi Front Bioeng Biotechnol Bioengineering and Biotechnology Nanofibers as elements for bioscaffolds are pushing the development of tissue engineering. In this study, tussah silk was mechanically disintegrated into nanofibers dispersed in aqueous solution which was cast to generate tussah silk fibroin (TSF) nanofiber mats. The effect of treatment time on the morphology, structure, and mechanical properties of nanofiber mats was examined. SEM indicated decreasing diameter of the nanofiber with shearing time, and the diameter of the nanofiber was 139.7 nm after 30 min treatment. These nanofiber mats exhibited excellent mechanical properties; the breaking strength increased from 26.31 to 72.68 MPa with the decrease of fiber diameter from 196.5 to 139.7 nm. The particulate debris was observed on protease XIV degraded nanofiber mats, and the weight loss was greater than 10% after 30 days in vitro degradation. The cell compatibility experiment confirmed adhesion and spreading of NIH-3T3 cells and enhanced cell proliferation on TSF nanofiber mats compared to that on Bombyx mori silk nanofiber mats. In conclusion, results indicate that TSF nanofiber mats prepared in this study are mechanically robust, slow biodegradable, and biocompatible materials, and have promising application in regenerative medicine. Frontiers Media S.A. 2021-12-02 /pmc/articles/PMC8677428/ /pubmed/34926415 http://dx.doi.org/10.3389/fbioe.2021.746016 Text en Copyright © 2021 Chen, Qin, Lu, Zhang and Zuo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Chen, Ming
Qin, Jianzhong
Lu, Shijun
Zhang, Feng
Zuo, Baoqi
Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications
title Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications
title_full Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications
title_fullStr Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications
title_full_unstemmed Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications
title_short Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications
title_sort robust nanofiber mats exfoliated from tussah silk for potential biomedical applications
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8677428/
https://www.ncbi.nlm.nih.gov/pubmed/34926415
http://dx.doi.org/10.3389/fbioe.2021.746016
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