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Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications
Nanofibers as elements for bioscaffolds are pushing the development of tissue engineering. In this study, tussah silk was mechanically disintegrated into nanofibers dispersed in aqueous solution which was cast to generate tussah silk fibroin (TSF) nanofiber mats. The effect of treatment time on the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8677428/ https://www.ncbi.nlm.nih.gov/pubmed/34926415 http://dx.doi.org/10.3389/fbioe.2021.746016 |
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author | Chen, Ming Qin, Jianzhong Lu, Shijun Zhang, Feng Zuo, Baoqi |
author_facet | Chen, Ming Qin, Jianzhong Lu, Shijun Zhang, Feng Zuo, Baoqi |
author_sort | Chen, Ming |
collection | PubMed |
description | Nanofibers as elements for bioscaffolds are pushing the development of tissue engineering. In this study, tussah silk was mechanically disintegrated into nanofibers dispersed in aqueous solution which was cast to generate tussah silk fibroin (TSF) nanofiber mats. The effect of treatment time on the morphology, structure, and mechanical properties of nanofiber mats was examined. SEM indicated decreasing diameter of the nanofiber with shearing time, and the diameter of the nanofiber was 139.7 nm after 30 min treatment. These nanofiber mats exhibited excellent mechanical properties; the breaking strength increased from 26.31 to 72.68 MPa with the decrease of fiber diameter from 196.5 to 139.7 nm. The particulate debris was observed on protease XIV degraded nanofiber mats, and the weight loss was greater than 10% after 30 days in vitro degradation. The cell compatibility experiment confirmed adhesion and spreading of NIH-3T3 cells and enhanced cell proliferation on TSF nanofiber mats compared to that on Bombyx mori silk nanofiber mats. In conclusion, results indicate that TSF nanofiber mats prepared in this study are mechanically robust, slow biodegradable, and biocompatible materials, and have promising application in regenerative medicine. |
format | Online Article Text |
id | pubmed-8677428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86774282021-12-17 Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications Chen, Ming Qin, Jianzhong Lu, Shijun Zhang, Feng Zuo, Baoqi Front Bioeng Biotechnol Bioengineering and Biotechnology Nanofibers as elements for bioscaffolds are pushing the development of tissue engineering. In this study, tussah silk was mechanically disintegrated into nanofibers dispersed in aqueous solution which was cast to generate tussah silk fibroin (TSF) nanofiber mats. The effect of treatment time on the morphology, structure, and mechanical properties of nanofiber mats was examined. SEM indicated decreasing diameter of the nanofiber with shearing time, and the diameter of the nanofiber was 139.7 nm after 30 min treatment. These nanofiber mats exhibited excellent mechanical properties; the breaking strength increased from 26.31 to 72.68 MPa with the decrease of fiber diameter from 196.5 to 139.7 nm. The particulate debris was observed on protease XIV degraded nanofiber mats, and the weight loss was greater than 10% after 30 days in vitro degradation. The cell compatibility experiment confirmed adhesion and spreading of NIH-3T3 cells and enhanced cell proliferation on TSF nanofiber mats compared to that on Bombyx mori silk nanofiber mats. In conclusion, results indicate that TSF nanofiber mats prepared in this study are mechanically robust, slow biodegradable, and biocompatible materials, and have promising application in regenerative medicine. Frontiers Media S.A. 2021-12-02 /pmc/articles/PMC8677428/ /pubmed/34926415 http://dx.doi.org/10.3389/fbioe.2021.746016 Text en Copyright © 2021 Chen, Qin, Lu, Zhang and Zuo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Chen, Ming Qin, Jianzhong Lu, Shijun Zhang, Feng Zuo, Baoqi Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications |
title | Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications |
title_full | Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications |
title_fullStr | Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications |
title_full_unstemmed | Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications |
title_short | Robust Nanofiber Mats Exfoliated From Tussah Silk for Potential Biomedical Applications |
title_sort | robust nanofiber mats exfoliated from tussah silk for potential biomedical applications |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8677428/ https://www.ncbi.nlm.nih.gov/pubmed/34926415 http://dx.doi.org/10.3389/fbioe.2021.746016 |
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