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Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica
During plant interaction, endophytes provide benefits to the host plant. Endophytes also contribute a variety of structural attributes with biological potential. Nigrospora sphaerica, which produces phomalactone from Adiantum philippense L., was subjected to epigenetic modification. High-performance...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678036/ https://www.ncbi.nlm.nih.gov/pubmed/34925254 http://dx.doi.org/10.3389/fmicb.2021.730355 |
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author | Ramesha, Kolathuru Puttamadaiah Chandra Mohana, Nagabhushana Chandra Nayaka, Siddaiah Satish, Sreedharamurthy |
author_facet | Ramesha, Kolathuru Puttamadaiah Chandra Mohana, Nagabhushana Chandra Nayaka, Siddaiah Satish, Sreedharamurthy |
author_sort | Ramesha, Kolathuru Puttamadaiah |
collection | PubMed |
description | During plant interaction, endophytes provide benefits to the host plant. Endophytes also contribute a variety of structural attributes with biological potential. Nigrospora sphaerica, which produces phomalactone from Adiantum philippense L., was subjected to epigenetic modification. High-performance liquid chromatography (HPLC) and Gas chromatography-mass spectrometry (GCMS) analysis were used to determine secondary metabolite profiling. Epigenetic modifiers like DNA Methyltransferase (DNMT) and Histone deacetylase (HDAC) inhibitors increased the expression of biosynthetic pathways. The activation of new metabolites was observed as a result of the activation of cryptic biosynthetic gene clusters, as well as the silencing of phomalactone in some treatments. When compared to DNMT treatments, HDAC treatments showed a significant increase in cryptic metabolite induction. The induction of cryptic metabolites with biological significance by HDAC treatment is supported by our findings. |
format | Online Article Text |
id | pubmed-8678036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86780362021-12-18 Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica Ramesha, Kolathuru Puttamadaiah Chandra Mohana, Nagabhushana Chandra Nayaka, Siddaiah Satish, Sreedharamurthy Front Microbiol Microbiology During plant interaction, endophytes provide benefits to the host plant. Endophytes also contribute a variety of structural attributes with biological potential. Nigrospora sphaerica, which produces phomalactone from Adiantum philippense L., was subjected to epigenetic modification. High-performance liquid chromatography (HPLC) and Gas chromatography-mass spectrometry (GCMS) analysis were used to determine secondary metabolite profiling. Epigenetic modifiers like DNA Methyltransferase (DNMT) and Histone deacetylase (HDAC) inhibitors increased the expression of biosynthetic pathways. The activation of new metabolites was observed as a result of the activation of cryptic biosynthetic gene clusters, as well as the silencing of phomalactone in some treatments. When compared to DNMT treatments, HDAC treatments showed a significant increase in cryptic metabolite induction. The induction of cryptic metabolites with biological significance by HDAC treatment is supported by our findings. Frontiers Media S.A. 2021-12-02 /pmc/articles/PMC8678036/ /pubmed/34925254 http://dx.doi.org/10.3389/fmicb.2021.730355 Text en Copyright © 2021 Ramesha, Chandra Mohana, Chandra Nayaka and Satish. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Ramesha, Kolathuru Puttamadaiah Chandra Mohana, Nagabhushana Chandra Nayaka, Siddaiah Satish, Sreedharamurthy Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica |
title | Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica |
title_full | Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica |
title_fullStr | Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica |
title_full_unstemmed | Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica |
title_short | Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica |
title_sort | epigenetic modifiers revamp secondary metabolite production in endophytic nigrospora sphaerica |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678036/ https://www.ncbi.nlm.nih.gov/pubmed/34925254 http://dx.doi.org/10.3389/fmicb.2021.730355 |
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