High-content single-cell combinatorial indexing

Single-cell combinatorial indexing (sci) with transposase-based library construction increases the throughput of single-cell genomics assays but produces sparse coverage in terms of usable reads per cell. We develop symmetrical strand sci (‘s3’), a uracil-based adapter switching approach that improv...

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Detalles Bibliográficos
Autores principales: Mulqueen, Ryan M., Pokholok, Dmitry, O’Connell, Brendan L., Thornton, Casey A., Zhang, Fan, O’Roak, Brian J., Link, Jason, Yardımcı, Galip Gürkan, Sears, Rosalie C., Steemers, Frank J., Adey, Andrew C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678206/
https://www.ncbi.nlm.nih.gov/pubmed/34226710
http://dx.doi.org/10.1038/s41587-021-00962-z

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