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Photoluminescence-Based Bioassay With Cysteamine-Capped TiO(2) Nanoparticles for the Selective Recognition of N-Acyl Homoserine Lactones

Currently available diagnostic procedures for infections are laborious and time-consuming, resulting in a substantial financial burden by increasing morbidity, increased costs of hospitalization, and mortality. Therefore, innovative approaches to design diagnostic biomarkers are imperative to assist...

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Detalles Bibliográficos
Autores principales: Vasudevan, Sahana, Srinivasan, Parthasarathy, Neelakantan, Prasanna, Rayappan, John Bosco Balaguru, Solomon, Adline Princy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678280/
https://www.ncbi.nlm.nih.gov/pubmed/34926417
http://dx.doi.org/10.3389/fbioe.2021.750933
Descripción
Sumario:Currently available diagnostic procedures for infections are laborious and time-consuming, resulting in a substantial financial burden by increasing morbidity, increased costs of hospitalization, and mortality. Therefore, innovative approaches to design diagnostic biomarkers are imperative to assist in the rapid and sensitive diagnosis of microbial infections. Acyl homoserine lactones (AHLs) are ubiquitous bacterial signaling molecules that are found to be significantly upregulated in infected sites. In this pioneering work, we have developed a simple photoluminescence-based assay using cysteamine-capped titanium oxide (TiO(2)) nanoparticles for AHL detection. The PL intensity variation of the oxygen defect state of TiO(2) was used for the biosensing measurements. The bioassays were validated using two well-studied AHL molecules (C4-HSL and 3-oxo-C12 HSL) of an important human pathogen, Pseudomonas aeruginosa. The developed system has a maximum relative response of 98%. Furthermore, the efficacy of the system in simulated host urine using an artificial urine medium showed a linear detection range of 10–160 nM. Also, we confirmed the relative response and specificity of the system in detecting AHLs produced by P. aeruginosa in a temporal manner.