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Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication
Bioprinting emerges as a powerful flexible approach for tissue engineering with prospective capability to produce tissue on demand, including biomimetic hollow-core fiber structures. In spite of significance for tissue engineering, hollow-core structures proved difficult to fabricate, with the exist...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678487/ https://www.ncbi.nlm.nih.gov/pubmed/34926429 http://dx.doi.org/10.3389/fbioe.2021.783834 |
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author | Savelyev, Alexander G. Sochilina, Anastasia V. Akasov, Roman A. Mironov, Anton V. Kapitannikova, Alina Yu. Borodina, Tatiana N. Sholina, Natalya V. Khaydukov, Kirill V. Zvyagin, Andrei V. Generalova, Alla N. Khaydukov, Evgeny V. |
author_facet | Savelyev, Alexander G. Sochilina, Anastasia V. Akasov, Roman A. Mironov, Anton V. Kapitannikova, Alina Yu. Borodina, Tatiana N. Sholina, Natalya V. Khaydukov, Kirill V. Zvyagin, Andrei V. Generalova, Alla N. Khaydukov, Evgeny V. |
author_sort | Savelyev, Alexander G. |
collection | PubMed |
description | Bioprinting emerges as a powerful flexible approach for tissue engineering with prospective capability to produce tissue on demand, including biomimetic hollow-core fiber structures. In spite of significance for tissue engineering, hollow-core structures proved difficult to fabricate, with the existing methods limited to multistage, time-consuming, and cumbersome procedures. Here, we report a versatile cell-friendly photopolymerization approach that enables single-step prototyping of hollow-core as well as solid-core hydrogel fibers initially loaded with living cells. This approach was implemented by extruding cell-laden hyaluronic acid glycidyl methacrylate hydrogel directly into aqueous solution containing free radicals generated by continuous blue light photoexcitation of the flavin mononucleotide/triethanolamine photoinitiator. Diffusion of free radicals from the solution to the extruded structure initiated cross-linking of the hydrogel, progressing from the structure surface inwards. Thus, the cross-linked wall is formed and its thickness is limited by penetration of free radicals in the hydrogel volume. After developing in water, the hollow-core fiber is formed with centimeter range of lengths. Amazingly, HaCaT cells embedded in the hydrogel successfully go through the fabrication procedure. The broad size ranges have been demonstrated: from solid core to 6% wall thickness of the outer diameter, which was variable from sub-millimeter to 6 mm, and Young’s modulus ∼1.6 ± 0.4 MPa. This new proof-of-concept fibers photofabrication approach opens lucrative opportunities for facile three-dimensional fabrication of hollow-core biostructures with controllable geometry. |
format | Online Article Text |
id | pubmed-8678487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86784872021-12-18 Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication Savelyev, Alexander G. Sochilina, Anastasia V. Akasov, Roman A. Mironov, Anton V. Kapitannikova, Alina Yu. Borodina, Tatiana N. Sholina, Natalya V. Khaydukov, Kirill V. Zvyagin, Andrei V. Generalova, Alla N. Khaydukov, Evgeny V. Front Bioeng Biotechnol Bioengineering and Biotechnology Bioprinting emerges as a powerful flexible approach for tissue engineering with prospective capability to produce tissue on demand, including biomimetic hollow-core fiber structures. In spite of significance for tissue engineering, hollow-core structures proved difficult to fabricate, with the existing methods limited to multistage, time-consuming, and cumbersome procedures. Here, we report a versatile cell-friendly photopolymerization approach that enables single-step prototyping of hollow-core as well as solid-core hydrogel fibers initially loaded with living cells. This approach was implemented by extruding cell-laden hyaluronic acid glycidyl methacrylate hydrogel directly into aqueous solution containing free radicals generated by continuous blue light photoexcitation of the flavin mononucleotide/triethanolamine photoinitiator. Diffusion of free radicals from the solution to the extruded structure initiated cross-linking of the hydrogel, progressing from the structure surface inwards. Thus, the cross-linked wall is formed and its thickness is limited by penetration of free radicals in the hydrogel volume. After developing in water, the hollow-core fiber is formed with centimeter range of lengths. Amazingly, HaCaT cells embedded in the hydrogel successfully go through the fabrication procedure. The broad size ranges have been demonstrated: from solid core to 6% wall thickness of the outer diameter, which was variable from sub-millimeter to 6 mm, and Young’s modulus ∼1.6 ± 0.4 MPa. This new proof-of-concept fibers photofabrication approach opens lucrative opportunities for facile three-dimensional fabrication of hollow-core biostructures with controllable geometry. Frontiers Media S.A. 2021-12-03 /pmc/articles/PMC8678487/ /pubmed/34926429 http://dx.doi.org/10.3389/fbioe.2021.783834 Text en Copyright © 2021 Savelyev, Sochilina, Akasov, Mironov, Kapitannikova, Borodina, Sholina, Khaydukov, Zvyagin, Generalova and Khaydukov. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Savelyev, Alexander G. Sochilina, Anastasia V. Akasov, Roman A. Mironov, Anton V. Kapitannikova, Alina Yu. Borodina, Tatiana N. Sholina, Natalya V. Khaydukov, Kirill V. Zvyagin, Andrei V. Generalova, Alla N. Khaydukov, Evgeny V. Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication |
title | Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication |
title_full | Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication |
title_fullStr | Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication |
title_full_unstemmed | Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication |
title_short | Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication |
title_sort | facile cell-friendly hollow-core fiber diffusion-limited photofabrication |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678487/ https://www.ncbi.nlm.nih.gov/pubmed/34926429 http://dx.doi.org/10.3389/fbioe.2021.783834 |
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