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Abnormal Aggregation of Invasive Cancer Cells Induced by Collective Polarization and ECM-Mediated Mechanical Coupling in Coculture Systems

Studies on pattern formation in coculture cell systems can provide insights into many physiological and pathological processes. Here, we investigate how the extracellular matrix (ECM) may influence the patterning in coculture systems. The model coculture system we use is composed of highly motile in...

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Autores principales: Wang, Xiaochen, Chen, Shaohua, Nan, Hanqing, Liu, Ruchuan, Ding, Yu, Song, Kena, Shuai, Jianwei, Fan, Qihui, Zheng, Yu, Ye, Fangfu, Jiao, Yang, Liu, Liyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AAAS 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678614/
https://www.ncbi.nlm.nih.gov/pubmed/34957406
http://dx.doi.org/10.34133/2021/9893131
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author Wang, Xiaochen
Chen, Shaohua
Nan, Hanqing
Liu, Ruchuan
Ding, Yu
Song, Kena
Shuai, Jianwei
Fan, Qihui
Zheng, Yu
Ye, Fangfu
Jiao, Yang
Liu, Liyu
author_facet Wang, Xiaochen
Chen, Shaohua
Nan, Hanqing
Liu, Ruchuan
Ding, Yu
Song, Kena
Shuai, Jianwei
Fan, Qihui
Zheng, Yu
Ye, Fangfu
Jiao, Yang
Liu, Liyu
author_sort Wang, Xiaochen
collection PubMed
description Studies on pattern formation in coculture cell systems can provide insights into many physiological and pathological processes. Here, we investigate how the extracellular matrix (ECM) may influence the patterning in coculture systems. The model coculture system we use is composed of highly motile invasive breast cancer cells, initially mixed with inert nonmetastatic cells on a 2D substrate and covered with a Matrigel layer introduced to mimic ECM. We observe that the invasive cells exhibit persistent centripetal motion and yield abnormal aggregation, rather than random spreading, due to a “collective pulling” effect resulting from ECM-mediated transmission of active contractile forces generated by the polarized migration of the invasive cells along the vertical direction. The mechanism we report may open a new window for the understanding of biological processes that involve multiple types of cells.
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spelling pubmed-86786142021-12-23 Abnormal Aggregation of Invasive Cancer Cells Induced by Collective Polarization and ECM-Mediated Mechanical Coupling in Coculture Systems Wang, Xiaochen Chen, Shaohua Nan, Hanqing Liu, Ruchuan Ding, Yu Song, Kena Shuai, Jianwei Fan, Qihui Zheng, Yu Ye, Fangfu Jiao, Yang Liu, Liyu Research (Wash D C) Research Article Studies on pattern formation in coculture cell systems can provide insights into many physiological and pathological processes. Here, we investigate how the extracellular matrix (ECM) may influence the patterning in coculture systems. The model coculture system we use is composed of highly motile invasive breast cancer cells, initially mixed with inert nonmetastatic cells on a 2D substrate and covered with a Matrigel layer introduced to mimic ECM. We observe that the invasive cells exhibit persistent centripetal motion and yield abnormal aggregation, rather than random spreading, due to a “collective pulling” effect resulting from ECM-mediated transmission of active contractile forces generated by the polarized migration of the invasive cells along the vertical direction. The mechanism we report may open a new window for the understanding of biological processes that involve multiple types of cells. AAAS 2021-12-08 /pmc/articles/PMC8678614/ /pubmed/34957406 http://dx.doi.org/10.34133/2021/9893131 Text en Copyright © 2021 Xiaochen Wang et al. https://creativecommons.org/licenses/by/4.0/Exclusive Licensee Science and Technology Review Publishing House. Distributed under a Creative Commons Attribution License (CC BY 4.0).
spellingShingle Research Article
Wang, Xiaochen
Chen, Shaohua
Nan, Hanqing
Liu, Ruchuan
Ding, Yu
Song, Kena
Shuai, Jianwei
Fan, Qihui
Zheng, Yu
Ye, Fangfu
Jiao, Yang
Liu, Liyu
Abnormal Aggregation of Invasive Cancer Cells Induced by Collective Polarization and ECM-Mediated Mechanical Coupling in Coculture Systems
title Abnormal Aggregation of Invasive Cancer Cells Induced by Collective Polarization and ECM-Mediated Mechanical Coupling in Coculture Systems
title_full Abnormal Aggregation of Invasive Cancer Cells Induced by Collective Polarization and ECM-Mediated Mechanical Coupling in Coculture Systems
title_fullStr Abnormal Aggregation of Invasive Cancer Cells Induced by Collective Polarization and ECM-Mediated Mechanical Coupling in Coculture Systems
title_full_unstemmed Abnormal Aggregation of Invasive Cancer Cells Induced by Collective Polarization and ECM-Mediated Mechanical Coupling in Coculture Systems
title_short Abnormal Aggregation of Invasive Cancer Cells Induced by Collective Polarization and ECM-Mediated Mechanical Coupling in Coculture Systems
title_sort abnormal aggregation of invasive cancer cells induced by collective polarization and ecm-mediated mechanical coupling in coculture systems
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678614/
https://www.ncbi.nlm.nih.gov/pubmed/34957406
http://dx.doi.org/10.34133/2021/9893131
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