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miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin
Osteosarcoma (OS), characterized by high morbidity and mortality, is the most common bone malignancy worldwide. MicroRNAs (miRNAs) play a crucial role in the initiation and development of OS. The purpose of this study was to investigate the roles of miR-1270 in OS. RT-qPCR and Western blot were appl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PAGEPress Publications, Pavia, Italy
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678625/ https://www.ncbi.nlm.nih.gov/pubmed/34873899 http://dx.doi.org/10.4081/ejh.2021.3237 |
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author | Liu, Yang Guo, Weichun Fang, Shuo He, Bin Li, Xiaohai Fan, Li |
author_facet | Liu, Yang Guo, Weichun Fang, Shuo He, Bin Li, Xiaohai Fan, Li |
author_sort | Liu, Yang |
collection | PubMed |
description | Osteosarcoma (OS), characterized by high morbidity and mortality, is the most common bone malignancy worldwide. MicroRNAs (miRNAs) play a crucial role in the initiation and development of OS. The purpose of this study was to investigate the roles of miR-1270 in OS. RT-qPCR and Western blot were applied to detect the mRNA and protein level, respectively. CCK-8, colony formation, and TUNEL assays were conducted to determine the cell viability, proliferation, and apoptosis of OS cells. Wound healing and transwell assay were performed to detect the migration and invasion ability of OS cells. Bioinformatics analysis and dual-luciferase reporter assay were used to predict the target genes of miR-1270. Tumor xenograft in vivo assay was carried out to determine miR-1270 effect on the tumor size, volume, and weight. In this study, miR-1270 was overexpressed in OS tissues and cells. However, miR-1270 knockdown inhibited the proliferation, migration and invasion, and promoted the OS cells’ apoptosis. Mechanistically, cingulin (CGN) was predicted and proved to be a target of miR-1270 and partially alleviated the effects of miR-1270 on the proliferation, migration and invasion ability of OS cells. Taken together, knockdown of miR-1270 may inhibit the development of OS via targeting CGN. This finding may provide a novel therapeutic strategy for OS. |
format | Online Article Text |
id | pubmed-8678625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PAGEPress Publications, Pavia, Italy |
record_format | MEDLINE/PubMed |
spelling | pubmed-86786252022-01-06 miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin Liu, Yang Guo, Weichun Fang, Shuo He, Bin Li, Xiaohai Fan, Li Eur J Histochem Article Osteosarcoma (OS), characterized by high morbidity and mortality, is the most common bone malignancy worldwide. MicroRNAs (miRNAs) play a crucial role in the initiation and development of OS. The purpose of this study was to investigate the roles of miR-1270 in OS. RT-qPCR and Western blot were applied to detect the mRNA and protein level, respectively. CCK-8, colony formation, and TUNEL assays were conducted to determine the cell viability, proliferation, and apoptosis of OS cells. Wound healing and transwell assay were performed to detect the migration and invasion ability of OS cells. Bioinformatics analysis and dual-luciferase reporter assay were used to predict the target genes of miR-1270. Tumor xenograft in vivo assay was carried out to determine miR-1270 effect on the tumor size, volume, and weight. In this study, miR-1270 was overexpressed in OS tissues and cells. However, miR-1270 knockdown inhibited the proliferation, migration and invasion, and promoted the OS cells’ apoptosis. Mechanistically, cingulin (CGN) was predicted and proved to be a target of miR-1270 and partially alleviated the effects of miR-1270 on the proliferation, migration and invasion ability of OS cells. Taken together, knockdown of miR-1270 may inhibit the development of OS via targeting CGN. This finding may provide a novel therapeutic strategy for OS. PAGEPress Publications, Pavia, Italy 2021-12-07 /pmc/articles/PMC8678625/ /pubmed/34873899 http://dx.doi.org/10.4081/ejh.2021.3237 Text en ©Copyright: the Author(s) https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Liu, Yang Guo, Weichun Fang, Shuo He, Bin Li, Xiaohai Fan, Li miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin |
title | miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin |
title_full | miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin |
title_fullStr | miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin |
title_full_unstemmed | miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin |
title_short | miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin |
title_sort | mir-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678625/ https://www.ncbi.nlm.nih.gov/pubmed/34873899 http://dx.doi.org/10.4081/ejh.2021.3237 |
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