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miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin

Osteosarcoma (OS), characterized by high morbidity and mortality, is the most common bone malignancy worldwide. MicroRNAs (miRNAs) play a crucial role in the initiation and development of OS. The purpose of this study was to investigate the roles of miR-1270 in OS. RT-qPCR and Western blot were appl...

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Autores principales: Liu, Yang, Guo, Weichun, Fang, Shuo, He, Bin, Li, Xiaohai, Fan, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678625/
https://www.ncbi.nlm.nih.gov/pubmed/34873899
http://dx.doi.org/10.4081/ejh.2021.3237
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author Liu, Yang
Guo, Weichun
Fang, Shuo
He, Bin
Li, Xiaohai
Fan, Li
author_facet Liu, Yang
Guo, Weichun
Fang, Shuo
He, Bin
Li, Xiaohai
Fan, Li
author_sort Liu, Yang
collection PubMed
description Osteosarcoma (OS), characterized by high morbidity and mortality, is the most common bone malignancy worldwide. MicroRNAs (miRNAs) play a crucial role in the initiation and development of OS. The purpose of this study was to investigate the roles of miR-1270 in OS. RT-qPCR and Western blot were applied to detect the mRNA and protein level, respectively. CCK-8, colony formation, and TUNEL assays were conducted to determine the cell viability, proliferation, and apoptosis of OS cells. Wound healing and transwell assay were performed to detect the migration and invasion ability of OS cells. Bioinformatics analysis and dual-luciferase reporter assay were used to predict the target genes of miR-1270. Tumor xenograft in vivo assay was carried out to determine miR-1270 effect on the tumor size, volume, and weight. In this study, miR-1270 was overexpressed in OS tissues and cells. However, miR-1270 knockdown inhibited the proliferation, migration and invasion, and promoted the OS cells’ apoptosis. Mechanistically, cingulin (CGN) was predicted and proved to be a target of miR-1270 and partially alleviated the effects of miR-1270 on the proliferation, migration and invasion ability of OS cells. Taken together, knockdown of miR-1270 may inhibit the development of OS via targeting CGN. This finding may provide a novel therapeutic strategy for OS.
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spelling pubmed-86786252022-01-06 miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin Liu, Yang Guo, Weichun Fang, Shuo He, Bin Li, Xiaohai Fan, Li Eur J Histochem Article Osteosarcoma (OS), characterized by high morbidity and mortality, is the most common bone malignancy worldwide. MicroRNAs (miRNAs) play a crucial role in the initiation and development of OS. The purpose of this study was to investigate the roles of miR-1270 in OS. RT-qPCR and Western blot were applied to detect the mRNA and protein level, respectively. CCK-8, colony formation, and TUNEL assays were conducted to determine the cell viability, proliferation, and apoptosis of OS cells. Wound healing and transwell assay were performed to detect the migration and invasion ability of OS cells. Bioinformatics analysis and dual-luciferase reporter assay were used to predict the target genes of miR-1270. Tumor xenograft in vivo assay was carried out to determine miR-1270 effect on the tumor size, volume, and weight. In this study, miR-1270 was overexpressed in OS tissues and cells. However, miR-1270 knockdown inhibited the proliferation, migration and invasion, and promoted the OS cells’ apoptosis. Mechanistically, cingulin (CGN) was predicted and proved to be a target of miR-1270 and partially alleviated the effects of miR-1270 on the proliferation, migration and invasion ability of OS cells. Taken together, knockdown of miR-1270 may inhibit the development of OS via targeting CGN. This finding may provide a novel therapeutic strategy for OS. PAGEPress Publications, Pavia, Italy 2021-12-07 /pmc/articles/PMC8678625/ /pubmed/34873899 http://dx.doi.org/10.4081/ejh.2021.3237 Text en ©Copyright: the Author(s) https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Liu, Yang
Guo, Weichun
Fang, Shuo
He, Bin
Li, Xiaohai
Fan, Li
miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin
title miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin
title_full miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin
title_fullStr miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin
title_full_unstemmed miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin
title_short miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin
title_sort mir-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678625/
https://www.ncbi.nlm.nih.gov/pubmed/34873899
http://dx.doi.org/10.4081/ejh.2021.3237
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