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Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat

The inositol phospholipid signaling system mediates plant growth, development, and responses to adverse conditions. Diacylglycerol kinase (DGK) is one of the key enzymes in the phosphoinositide-cycle (PI-cycle), which catalyzes the phosphorylation of diacylglycerol (DAG) to form phosphatidic acid (P...

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Autores principales: Jia, Xiaowei, Si, Xuyang, Jia, Yangyang, Zhang, Hongyan, Tian, Shijun, Li, Wenjing, Zhang, Ke, Pan, Yanyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8679913/
https://www.ncbi.nlm.nih.gov/pubmed/34993014
http://dx.doi.org/10.7717/peerj.12480
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author Jia, Xiaowei
Si, Xuyang
Jia, Yangyang
Zhang, Hongyan
Tian, Shijun
Li, Wenjing
Zhang, Ke
Pan, Yanyun
author_facet Jia, Xiaowei
Si, Xuyang
Jia, Yangyang
Zhang, Hongyan
Tian, Shijun
Li, Wenjing
Zhang, Ke
Pan, Yanyun
author_sort Jia, Xiaowei
collection PubMed
description The inositol phospholipid signaling system mediates plant growth, development, and responses to adverse conditions. Diacylglycerol kinase (DGK) is one of the key enzymes in the phosphoinositide-cycle (PI-cycle), which catalyzes the phosphorylation of diacylglycerol (DAG) to form phosphatidic acid (PA). To date, comprehensive genomic and functional analyses of DGKs have not been reported in wheat. In this study, 24 DGK gene family members from the wheat genome (TaDGKs) were identified and analyzed. Each putative protein was found to consist of a DGK catalytic domain and an accessory domain. The analyses of phylogenetic and gene structure analyses revealed that each TaDGK gene could be grouped into clusters I, II, or III. In each phylogenetic subgroup, the TaDGKs demonstrated high conservation of functional domains, for example, of gene structure and amino acid sequences. Four coding sequences were then cloned from Chinese Spring wheat. Expression analysis of these four genes revealed that each had a unique spatial and developmental expression pattern, indicating their functional diversification across wheat growth and development processes. Additionally, TaDGKs were also prominently up-regulated under salt and drought stresses, suggesting their possible roles in dealing with adverse environmental conditions. Further cis-regulatory elements analysis elucidated transcriptional regulation and potential biological functions. These results provide valuable information for understanding the putative functions of DGKs in wheat and support deeper functional analysis of this pivotal gene family. The 24 TaDGKs identified and analyzed in this study provide a strong foundation for further exploration of the biological function and regulatory mechanisms of TaDGKs in response to environmental stimuli.
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spelling pubmed-86799132022-01-05 Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat Jia, Xiaowei Si, Xuyang Jia, Yangyang Zhang, Hongyan Tian, Shijun Li, Wenjing Zhang, Ke Pan, Yanyun PeerJ Agricultural Science The inositol phospholipid signaling system mediates plant growth, development, and responses to adverse conditions. Diacylglycerol kinase (DGK) is one of the key enzymes in the phosphoinositide-cycle (PI-cycle), which catalyzes the phosphorylation of diacylglycerol (DAG) to form phosphatidic acid (PA). To date, comprehensive genomic and functional analyses of DGKs have not been reported in wheat. In this study, 24 DGK gene family members from the wheat genome (TaDGKs) were identified and analyzed. Each putative protein was found to consist of a DGK catalytic domain and an accessory domain. The analyses of phylogenetic and gene structure analyses revealed that each TaDGK gene could be grouped into clusters I, II, or III. In each phylogenetic subgroup, the TaDGKs demonstrated high conservation of functional domains, for example, of gene structure and amino acid sequences. Four coding sequences were then cloned from Chinese Spring wheat. Expression analysis of these four genes revealed that each had a unique spatial and developmental expression pattern, indicating their functional diversification across wheat growth and development processes. Additionally, TaDGKs were also prominently up-regulated under salt and drought stresses, suggesting their possible roles in dealing with adverse environmental conditions. Further cis-regulatory elements analysis elucidated transcriptional regulation and potential biological functions. These results provide valuable information for understanding the putative functions of DGKs in wheat and support deeper functional analysis of this pivotal gene family. The 24 TaDGKs identified and analyzed in this study provide a strong foundation for further exploration of the biological function and regulatory mechanisms of TaDGKs in response to environmental stimuli. PeerJ Inc. 2021-12-14 /pmc/articles/PMC8679913/ /pubmed/34993014 http://dx.doi.org/10.7717/peerj.12480 Text en ©2021 Jia et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Jia, Xiaowei
Si, Xuyang
Jia, Yangyang
Zhang, Hongyan
Tian, Shijun
Li, Wenjing
Zhang, Ke
Pan, Yanyun
Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat
title Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat
title_full Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat
title_fullStr Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat
title_full_unstemmed Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat
title_short Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat
title_sort genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8679913/
https://www.ncbi.nlm.nih.gov/pubmed/34993014
http://dx.doi.org/10.7717/peerj.12480
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