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Arginase II Promotes Intervertebral Disc Degeneration Through Exacerbating Senescence and Apoptosis Caused by Oxidative Stress and Inflammation via the NF-κB Pathway

Intervertebral disc degeneration (IDD) has been generally accepted as the major cause of low back pain (LBP), which imposes massive clinical and socioeconomic burdens. Previous studies have demonstrated that oxidative stress and inflammation-induced senescence and apoptosis of nucleus pulposus cells...

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Autores principales: Li, Fudong, Sun, Xiaofei, Zheng, Bing, Sun, Kaiqiang, Zhu, Jian, Ji, Chenglong, Lin, Feng, Huan, Le, Luo, Xi, Yan, Chen, Xu, Jiashun, Hong, Yun, Wang, Yuan, Xu, Ximing, Sun, Jingchuan, Song, Zheming, Kong, Fanqi, Shi, Jiangang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8679914/
https://www.ncbi.nlm.nih.gov/pubmed/34926442
http://dx.doi.org/10.3389/fcell.2021.737809
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author Li, Fudong
Sun, Xiaofei
Zheng, Bing
Sun, Kaiqiang
Zhu, Jian
Ji, Chenglong
Lin, Feng
Huan, Le
Luo, Xi
Yan, Chen
Xu, Jiashun
Hong, Yun
Wang, Yuan
Xu, Ximing
Sun, Jingchuan
Song, Zheming
Kong, Fanqi
Shi, Jiangang
author_facet Li, Fudong
Sun, Xiaofei
Zheng, Bing
Sun, Kaiqiang
Zhu, Jian
Ji, Chenglong
Lin, Feng
Huan, Le
Luo, Xi
Yan, Chen
Xu, Jiashun
Hong, Yun
Wang, Yuan
Xu, Ximing
Sun, Jingchuan
Song, Zheming
Kong, Fanqi
Shi, Jiangang
author_sort Li, Fudong
collection PubMed
description Intervertebral disc degeneration (IDD) has been generally accepted as the major cause of low back pain (LBP), which imposes massive clinical and socioeconomic burdens. Previous studies have demonstrated that oxidative stress and inflammation-induced senescence and apoptosis of nucleus pulposus cells (NPCs) are the main cellular processes that cause IDD. Arginase II (ARG2), an enzyme involved in a variety of pathological processes, including cellular senescence, apoptosis, oxidative stress, and inflammation, has been shown to promote degeneration in several degenerative diseases, including osteoarticular diseases. Based on previous studies, we hypothesized that ARG2 deficiency might be conducive to the treatment of IDD by inhibiting the dyshomeostasis of the extracellular matrix (ECM), and the oxidative stress and inflammatory response-induced senescence and apoptosis via NF-κB. In this study, we found that ARG2 deficiency inhibited senescence and apoptosis of NPCs, and degeneration of the ECM induced by oxidative stress and the inflammatory response. Similar results were found with the selective NF-κB pathway inhibitor JSH-23. In contrast, overexpression of ARG2 had the opposite effect. Taken together, our results suggest that ARG2 deficiency prevents IDD via NF-κB, and may therefore, be a potential therapeutic strategy for IDD.
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spelling pubmed-86799142021-12-18 Arginase II Promotes Intervertebral Disc Degeneration Through Exacerbating Senescence and Apoptosis Caused by Oxidative Stress and Inflammation via the NF-κB Pathway Li, Fudong Sun, Xiaofei Zheng, Bing Sun, Kaiqiang Zhu, Jian Ji, Chenglong Lin, Feng Huan, Le Luo, Xi Yan, Chen Xu, Jiashun Hong, Yun Wang, Yuan Xu, Ximing Sun, Jingchuan Song, Zheming Kong, Fanqi Shi, Jiangang Front Cell Dev Biol Cell and Developmental Biology Intervertebral disc degeneration (IDD) has been generally accepted as the major cause of low back pain (LBP), which imposes massive clinical and socioeconomic burdens. Previous studies have demonstrated that oxidative stress and inflammation-induced senescence and apoptosis of nucleus pulposus cells (NPCs) are the main cellular processes that cause IDD. Arginase II (ARG2), an enzyme involved in a variety of pathological processes, including cellular senescence, apoptosis, oxidative stress, and inflammation, has been shown to promote degeneration in several degenerative diseases, including osteoarticular diseases. Based on previous studies, we hypothesized that ARG2 deficiency might be conducive to the treatment of IDD by inhibiting the dyshomeostasis of the extracellular matrix (ECM), and the oxidative stress and inflammatory response-induced senescence and apoptosis via NF-κB. In this study, we found that ARG2 deficiency inhibited senescence and apoptosis of NPCs, and degeneration of the ECM induced by oxidative stress and the inflammatory response. Similar results were found with the selective NF-κB pathway inhibitor JSH-23. In contrast, overexpression of ARG2 had the opposite effect. Taken together, our results suggest that ARG2 deficiency prevents IDD via NF-κB, and may therefore, be a potential therapeutic strategy for IDD. Frontiers Media S.A. 2021-12-03 /pmc/articles/PMC8679914/ /pubmed/34926442 http://dx.doi.org/10.3389/fcell.2021.737809 Text en Copyright © 2021 Li, Sun, Zheng, Sun, Zhu, Ji, Lin, Huan, Luo, Yan, Xu, Hong, Wang, Xu, Sun, Song, Kong and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Li, Fudong
Sun, Xiaofei
Zheng, Bing
Sun, Kaiqiang
Zhu, Jian
Ji, Chenglong
Lin, Feng
Huan, Le
Luo, Xi
Yan, Chen
Xu, Jiashun
Hong, Yun
Wang, Yuan
Xu, Ximing
Sun, Jingchuan
Song, Zheming
Kong, Fanqi
Shi, Jiangang
Arginase II Promotes Intervertebral Disc Degeneration Through Exacerbating Senescence and Apoptosis Caused by Oxidative Stress and Inflammation via the NF-κB Pathway
title Arginase II Promotes Intervertebral Disc Degeneration Through Exacerbating Senescence and Apoptosis Caused by Oxidative Stress and Inflammation via the NF-κB Pathway
title_full Arginase II Promotes Intervertebral Disc Degeneration Through Exacerbating Senescence and Apoptosis Caused by Oxidative Stress and Inflammation via the NF-κB Pathway
title_fullStr Arginase II Promotes Intervertebral Disc Degeneration Through Exacerbating Senescence and Apoptosis Caused by Oxidative Stress and Inflammation via the NF-κB Pathway
title_full_unstemmed Arginase II Promotes Intervertebral Disc Degeneration Through Exacerbating Senescence and Apoptosis Caused by Oxidative Stress and Inflammation via the NF-κB Pathway
title_short Arginase II Promotes Intervertebral Disc Degeneration Through Exacerbating Senescence and Apoptosis Caused by Oxidative Stress and Inflammation via the NF-κB Pathway
title_sort arginase ii promotes intervertebral disc degeneration through exacerbating senescence and apoptosis caused by oxidative stress and inflammation via the nf-κb pathway
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8679914/
https://www.ncbi.nlm.nih.gov/pubmed/34926442
http://dx.doi.org/10.3389/fcell.2021.737809
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