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Maintenance of Physical Function in Adult and Older Adult Mice Using Aerobic Exercise

As we age, physical and neuromuscular function declines gradually. Exercise is a therapy to improve neuromuscular ability. Pre-clinical models are needed to understand molecular mechanisms contributing to age-associated functional decline and how exercise affects that downward trajectory. Our goal w...

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Autores principales: Graber, Ted, Pajski, Megan, Byrd, Christopher, Nadigama, Nainika, Fennel, Alyssa, Seguin, Emily, Seguin, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682566/
http://dx.doi.org/10.1093/geroni/igab046.3500
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author Graber, Ted
Pajski, Megan
Byrd, Christopher
Nadigama, Nainika
Fennel, Alyssa
Seguin, Emily
Seguin, Anna
author_facet Graber, Ted
Pajski, Megan
Byrd, Christopher
Nadigama, Nainika
Fennel, Alyssa
Seguin, Emily
Seguin, Anna
author_sort Graber, Ted
collection PubMed
description As we age, physical and neuromuscular function declines gradually. Exercise is a therapy to improve neuromuscular ability. Pre-clinical models are needed to understand molecular mechanisms contributing to age-associated functional decline and how exercise affects that downward trajectory. Our goal was to compare the differences in effects of two validated mouse models of endurance exercise designed to mimic human training studies: high intensity interval training (HIIT) and voluntary wheel running (VWR). We hypothesized that both adult and older adults (10 and 26 months old at end, respectively: 10m and 26m) would respond to both exercise regimens by improving or maintaining exercise/physical capacity, but that adult mice would benefit more. We randomly assigned male C57BL/6 mice into experimental groups: 10m: (VWR, HIIT, sedentary control, CON, n=8 per group), and 26m (VWR, n=8, HIIT, n=10). We measured functional ability (pre- and post-intervention) using CFAB (comprehensive functional assessment battery), our composite scoring system (grip strength, inverted cling, treadmill endurance, activity rate, rotarod), tracked body composition (EchoMRI), and measured muscle wet mass. We found that significant retention of ability (CFAB difference, repeated measures ANOVA, p<0.05) and fat percentage (ANOVA., %change: 10m: CON +125%, HIIT +101%, VWR +52%; 26m: VWR -42%, HIIT +26%, p<0.05) was promoted by both exercise modalities compared to control, and furthermore HIIT may have better efficacy in the adult versus the older mice. In conclusion, both exercises are valid models with derived benefits as expected in similar human studies. We anticipate future work using these models to undertake mechanistic investigations.
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spelling pubmed-86825662021-12-20 Maintenance of Physical Function in Adult and Older Adult Mice Using Aerobic Exercise Graber, Ted Pajski, Megan Byrd, Christopher Nadigama, Nainika Fennel, Alyssa Seguin, Emily Seguin, Anna Innov Aging Abstracts As we age, physical and neuromuscular function declines gradually. Exercise is a therapy to improve neuromuscular ability. Pre-clinical models are needed to understand molecular mechanisms contributing to age-associated functional decline and how exercise affects that downward trajectory. Our goal was to compare the differences in effects of two validated mouse models of endurance exercise designed to mimic human training studies: high intensity interval training (HIIT) and voluntary wheel running (VWR). We hypothesized that both adult and older adults (10 and 26 months old at end, respectively: 10m and 26m) would respond to both exercise regimens by improving or maintaining exercise/physical capacity, but that adult mice would benefit more. We randomly assigned male C57BL/6 mice into experimental groups: 10m: (VWR, HIIT, sedentary control, CON, n=8 per group), and 26m (VWR, n=8, HIIT, n=10). We measured functional ability (pre- and post-intervention) using CFAB (comprehensive functional assessment battery), our composite scoring system (grip strength, inverted cling, treadmill endurance, activity rate, rotarod), tracked body composition (EchoMRI), and measured muscle wet mass. We found that significant retention of ability (CFAB difference, repeated measures ANOVA, p<0.05) and fat percentage (ANOVA., %change: 10m: CON +125%, HIIT +101%, VWR +52%; 26m: VWR -42%, HIIT +26%, p<0.05) was promoted by both exercise modalities compared to control, and furthermore HIIT may have better efficacy in the adult versus the older mice. In conclusion, both exercises are valid models with derived benefits as expected in similar human studies. We anticipate future work using these models to undertake mechanistic investigations. Oxford University Press 2021-12-17 /pmc/articles/PMC8682566/ http://dx.doi.org/10.1093/geroni/igab046.3500 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of The Gerontological Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Abstracts
Graber, Ted
Pajski, Megan
Byrd, Christopher
Nadigama, Nainika
Fennel, Alyssa
Seguin, Emily
Seguin, Anna
Maintenance of Physical Function in Adult and Older Adult Mice Using Aerobic Exercise
title Maintenance of Physical Function in Adult and Older Adult Mice Using Aerobic Exercise
title_full Maintenance of Physical Function in Adult and Older Adult Mice Using Aerobic Exercise
title_fullStr Maintenance of Physical Function in Adult and Older Adult Mice Using Aerobic Exercise
title_full_unstemmed Maintenance of Physical Function in Adult and Older Adult Mice Using Aerobic Exercise
title_short Maintenance of Physical Function in Adult and Older Adult Mice Using Aerobic Exercise
title_sort maintenance of physical function in adult and older adult mice using aerobic exercise
topic Abstracts
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682566/
http://dx.doi.org/10.1093/geroni/igab046.3500
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