Cargando…

Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay

Pneumocystis pneumonia (PCP) and pulmonary toxoplasmosis (PT) are caused by Pneumocystis jirovecii and Toxoplasma gondii. The clinical symptoms and imaging of PCP and PT are indistinguishable. A duplex qPCR was developed to differentiate between these two pathogens. In testing 92 clinical samples to...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Yun, Wang, Fei, Wang, Chaoyue, Tang, Xinming, Liu, Xianyong, Li, Shaogang, Waterfield, Nicholas R., Wang, Wei, Suo, Xun, Yang, Guowei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682901/
https://www.ncbi.nlm.nih.gov/pubmed/34919557
http://dx.doi.org/10.1371/journal.pntd.0010025
_version_ 1784617310195023872
author Wu, Yun
Wang, Fei
Wang, Chaoyue
Tang, Xinming
Liu, Xianyong
Li, Shaogang
Waterfield, Nicholas R.
Wang, Wei
Suo, Xun
Yang, Guowei
author_facet Wu, Yun
Wang, Fei
Wang, Chaoyue
Tang, Xinming
Liu, Xianyong
Li, Shaogang
Waterfield, Nicholas R.
Wang, Wei
Suo, Xun
Yang, Guowei
author_sort Wu, Yun
collection PubMed
description Pneumocystis pneumonia (PCP) and pulmonary toxoplasmosis (PT) are caused by Pneumocystis jirovecii and Toxoplasma gondii. The clinical symptoms and imaging of PCP and PT are indistinguishable. A duplex qPCR was developed to differentiate between these two pathogens. In testing 92 clinical samples to validate the performance of this method for P. jirovecii detection, it identified 31 positive samples for P. jirovecii infection, consistent with clinical diagnosis. Among the remainder of the 61 clinical samples with suspected PCP, yet showing as negative by the conventional PCR diagnosis approach, 6 of them proved positive using our new assay. Our new approach also produced similar results in identification of T. gondii infections, giving a result of 2 positive and 20 negative in clinical samples. An investigation was undertaken on the prevalence of P. jirovecii and T. gondii infections using 113 samples from lung infection patients. 9% (10/113) were shown to be positive with infections of P. jirovecii, 2% with T. gondii (2/113) and 5% (6/113) were co-infected with both pathogens. Although this duplex qPCR can detect individual P. jirovecii and T. gondii infection, and co-infection of both pathogens, further large-scale investigations are needed to validate its performance, especially in T. gondii detection. Our assay provides a rapid and accurate tool for PCP and PT diagnosis in immunocompromised population and clinical surveillance of these infections in patients with no immune defects.
format Online
Article
Text
id pubmed-8682901
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-86829012021-12-18 Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay Wu, Yun Wang, Fei Wang, Chaoyue Tang, Xinming Liu, Xianyong Li, Shaogang Waterfield, Nicholas R. Wang, Wei Suo, Xun Yang, Guowei PLoS Negl Trop Dis Research Article Pneumocystis pneumonia (PCP) and pulmonary toxoplasmosis (PT) are caused by Pneumocystis jirovecii and Toxoplasma gondii. The clinical symptoms and imaging of PCP and PT are indistinguishable. A duplex qPCR was developed to differentiate between these two pathogens. In testing 92 clinical samples to validate the performance of this method for P. jirovecii detection, it identified 31 positive samples for P. jirovecii infection, consistent with clinical diagnosis. Among the remainder of the 61 clinical samples with suspected PCP, yet showing as negative by the conventional PCR diagnosis approach, 6 of them proved positive using our new assay. Our new approach also produced similar results in identification of T. gondii infections, giving a result of 2 positive and 20 negative in clinical samples. An investigation was undertaken on the prevalence of P. jirovecii and T. gondii infections using 113 samples from lung infection patients. 9% (10/113) were shown to be positive with infections of P. jirovecii, 2% with T. gondii (2/113) and 5% (6/113) were co-infected with both pathogens. Although this duplex qPCR can detect individual P. jirovecii and T. gondii infection, and co-infection of both pathogens, further large-scale investigations are needed to validate its performance, especially in T. gondii detection. Our assay provides a rapid and accurate tool for PCP and PT diagnosis in immunocompromised population and clinical surveillance of these infections in patients with no immune defects. Public Library of Science 2021-12-17 /pmc/articles/PMC8682901/ /pubmed/34919557 http://dx.doi.org/10.1371/journal.pntd.0010025 Text en © 2021 Wu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wu, Yun
Wang, Fei
Wang, Chaoyue
Tang, Xinming
Liu, Xianyong
Li, Shaogang
Waterfield, Nicholas R.
Wang, Wei
Suo, Xun
Yang, Guowei
Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay
title Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay
title_full Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay
title_fullStr Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay
title_full_unstemmed Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay
title_short Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay
title_sort detection of pneumocystis jirovecii and toxoplasma gondii in patients with lung infections by a duplex qpcr assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682901/
https://www.ncbi.nlm.nih.gov/pubmed/34919557
http://dx.doi.org/10.1371/journal.pntd.0010025
work_keys_str_mv AT wuyun detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay
AT wangfei detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay
AT wangchaoyue detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay
AT tangxinming detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay
AT liuxianyong detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay
AT lishaogang detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay
AT waterfieldnicholasr detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay
AT wangwei detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay
AT suoxun detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay
AT yangguowei detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay