Cargando…
Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay
Pneumocystis pneumonia (PCP) and pulmonary toxoplasmosis (PT) are caused by Pneumocystis jirovecii and Toxoplasma gondii. The clinical symptoms and imaging of PCP and PT are indistinguishable. A duplex qPCR was developed to differentiate between these two pathogens. In testing 92 clinical samples to...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682901/ https://www.ncbi.nlm.nih.gov/pubmed/34919557 http://dx.doi.org/10.1371/journal.pntd.0010025 |
_version_ | 1784617310195023872 |
---|---|
author | Wu, Yun Wang, Fei Wang, Chaoyue Tang, Xinming Liu, Xianyong Li, Shaogang Waterfield, Nicholas R. Wang, Wei Suo, Xun Yang, Guowei |
author_facet | Wu, Yun Wang, Fei Wang, Chaoyue Tang, Xinming Liu, Xianyong Li, Shaogang Waterfield, Nicholas R. Wang, Wei Suo, Xun Yang, Guowei |
author_sort | Wu, Yun |
collection | PubMed |
description | Pneumocystis pneumonia (PCP) and pulmonary toxoplasmosis (PT) are caused by Pneumocystis jirovecii and Toxoplasma gondii. The clinical symptoms and imaging of PCP and PT are indistinguishable. A duplex qPCR was developed to differentiate between these two pathogens. In testing 92 clinical samples to validate the performance of this method for P. jirovecii detection, it identified 31 positive samples for P. jirovecii infection, consistent with clinical diagnosis. Among the remainder of the 61 clinical samples with suspected PCP, yet showing as negative by the conventional PCR diagnosis approach, 6 of them proved positive using our new assay. Our new approach also produced similar results in identification of T. gondii infections, giving a result of 2 positive and 20 negative in clinical samples. An investigation was undertaken on the prevalence of P. jirovecii and T. gondii infections using 113 samples from lung infection patients. 9% (10/113) were shown to be positive with infections of P. jirovecii, 2% with T. gondii (2/113) and 5% (6/113) were co-infected with both pathogens. Although this duplex qPCR can detect individual P. jirovecii and T. gondii infection, and co-infection of both pathogens, further large-scale investigations are needed to validate its performance, especially in T. gondii detection. Our assay provides a rapid and accurate tool for PCP and PT diagnosis in immunocompromised population and clinical surveillance of these infections in patients with no immune defects. |
format | Online Article Text |
id | pubmed-8682901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-86829012021-12-18 Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay Wu, Yun Wang, Fei Wang, Chaoyue Tang, Xinming Liu, Xianyong Li, Shaogang Waterfield, Nicholas R. Wang, Wei Suo, Xun Yang, Guowei PLoS Negl Trop Dis Research Article Pneumocystis pneumonia (PCP) and pulmonary toxoplasmosis (PT) are caused by Pneumocystis jirovecii and Toxoplasma gondii. The clinical symptoms and imaging of PCP and PT are indistinguishable. A duplex qPCR was developed to differentiate between these two pathogens. In testing 92 clinical samples to validate the performance of this method for P. jirovecii detection, it identified 31 positive samples for P. jirovecii infection, consistent with clinical diagnosis. Among the remainder of the 61 clinical samples with suspected PCP, yet showing as negative by the conventional PCR diagnosis approach, 6 of them proved positive using our new assay. Our new approach also produced similar results in identification of T. gondii infections, giving a result of 2 positive and 20 negative in clinical samples. An investigation was undertaken on the prevalence of P. jirovecii and T. gondii infections using 113 samples from lung infection patients. 9% (10/113) were shown to be positive with infections of P. jirovecii, 2% with T. gondii (2/113) and 5% (6/113) were co-infected with both pathogens. Although this duplex qPCR can detect individual P. jirovecii and T. gondii infection, and co-infection of both pathogens, further large-scale investigations are needed to validate its performance, especially in T. gondii detection. Our assay provides a rapid and accurate tool for PCP and PT diagnosis in immunocompromised population and clinical surveillance of these infections in patients with no immune defects. Public Library of Science 2021-12-17 /pmc/articles/PMC8682901/ /pubmed/34919557 http://dx.doi.org/10.1371/journal.pntd.0010025 Text en © 2021 Wu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wu, Yun Wang, Fei Wang, Chaoyue Tang, Xinming Liu, Xianyong Li, Shaogang Waterfield, Nicholas R. Wang, Wei Suo, Xun Yang, Guowei Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay |
title | Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay |
title_full | Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay |
title_fullStr | Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay |
title_full_unstemmed | Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay |
title_short | Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay |
title_sort | detection of pneumocystis jirovecii and toxoplasma gondii in patients with lung infections by a duplex qpcr assay |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682901/ https://www.ncbi.nlm.nih.gov/pubmed/34919557 http://dx.doi.org/10.1371/journal.pntd.0010025 |
work_keys_str_mv | AT wuyun detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay AT wangfei detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay AT wangchaoyue detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay AT tangxinming detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay AT liuxianyong detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay AT lishaogang detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay AT waterfieldnicholasr detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay AT wangwei detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay AT suoxun detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay AT yangguowei detectionofpneumocystisjiroveciiandtoxoplasmagondiiinpatientswithlunginfectionsbyaduplexqpcrassay |