Tolerance of pesticides and antibiotics among beneficial soil microbes recovered from contaminated rhizosphere of edible crops

A total of 45 beneficial soil bacterial isolates (15 each of Pseudomonas, Azotobacter and phosphate solubilizing bacteria: PSB) recovered from polluted rhizosphere soils were morphologically and biochemically characterized. Bacterial isolates produced indole-3-acetic acid (IAA), phenolate siderophores; SA (salicylic acid) and 2, 3-dihydroxy benzoic acid (2, 3-DHBA), 1-amino cyclopropane 1-carboxylate (ACC) deaminase, solubilised insoluble phosphate (Pi), secreted exopolysaccharides (EPS) and produced ammonia and cyanogenic compound (HCN). Isolates were tested for their tolerance ability against 12 different agrochemicals (chemical pesticides) and 14 antibiotics. Among Pseudomonas, isolate PS1 showed maximum (2183 µg mL(−1)) tolerance to all tested agrochemicals. Likewise, among all Azotobacter isolates (n = 15), AZ12 showed maximum (1766 µg mL(−1)) while AZ7 had lowest (950 µg mL(−1)) tolerance ability to all tested agrochemicals. Moreover, among phosphate solubilizing bacterial isolates, maximum (1970 µg mL(−1)) and minimum (1308 µg mL(−1)) tolerance to agrochemicals was represented by PSB8 and PSB13 isolates, respectively. The antibiotic sensitivity/resistance among isolates varied considerably. As an example, Pseudomonas spp. was susceptible to several antibiotics, and inhibition zone differed between 10 mm (polymyxin B) to 34 mm (nalidixic acid). Also, isolate PS2 showed resistance to erythromycin, ciprofloxacin, methicillin, novobiocin and penicillin. The resistance percentage to multiple antibiotics among Azotobacter isolates varied between 7 and 33%. Among PSB isolates, inhibition zone differed between 10 and 40 mm and maximum and minimum resistance percentage to multiple antibiotics was recorded as 47% and 20%, respectively. The persistence of pesticides in agricultural soil may contribute to an increase in multidrug resistance among soil microorganisms. In conclusion, plant growth promoting (PGP) substances releasing soil microorganisms comprising of inherent/intrinsic properties of pesticides tolerance and antibiotics resistance may provide an attractive, agronomically feasible, and long-term prospective alternative for the augmentation of edible crops. However, in future, more research is needed to uncover the molecular processes behind the development of pesticide tolerance and antibiotic resistance among soil microorganisms.