Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines

Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such as mass spectrometry and ribosome profiling, are sophi...

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Detalles Bibliográficos
Autores principales: Han, Cai, Sun, Linyu, Pan, Qi, Sun, Yumeng, Wang, Wentao, Chen, Yueqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683657/
https://www.ncbi.nlm.nih.gov/pubmed/34977682
http://dx.doi.org/10.1016/j.xpro.2021.101037
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author Han, Cai
Sun, Linyu
Pan, Qi
Sun, Yumeng
Wang, Wentao
Chen, Yueqin
author_facet Han, Cai
Sun, Linyu
Pan, Qi
Sun, Yumeng
Wang, Wentao
Chen, Yueqin
author_sort Han, Cai
collection PubMed
description Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such as mass spectrometry and ribosome profiling, are sophisticated and expensive. Here, we present an easy and cost-effective protocol to screen for potential micropeptide-encoding lncRNAs by polysome profiling in suspension cell lines. When combined with quantitative PCR, this protocol facilitates the identification of a number of translating lncRNAs simultaneously. For complete details on the use and execution of this protocol, please refer to Sun et al. (2021).
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spelling pubmed-86836572021-12-30 Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines Han, Cai Sun, Linyu Pan, Qi Sun, Yumeng Wang, Wentao Chen, Yueqin STAR Protoc Protocol Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such as mass spectrometry and ribosome profiling, are sophisticated and expensive. Here, we present an easy and cost-effective protocol to screen for potential micropeptide-encoding lncRNAs by polysome profiling in suspension cell lines. When combined with quantitative PCR, this protocol facilitates the identification of a number of translating lncRNAs simultaneously. For complete details on the use and execution of this protocol, please refer to Sun et al. (2021). Elsevier 2021-12-14 /pmc/articles/PMC8683657/ /pubmed/34977682 http://dx.doi.org/10.1016/j.xpro.2021.101037 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Han, Cai
Sun, Linyu
Pan, Qi
Sun, Yumeng
Wang, Wentao
Chen, Yueqin
Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines
title Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines
title_full Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines
title_fullStr Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines
title_full_unstemmed Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines
title_short Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines
title_sort polysome profiling followed by quantitative pcr for identifying potential micropeptide encoding long non-coding rnas in suspension cell lines
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683657/
https://www.ncbi.nlm.nih.gov/pubmed/34977682
http://dx.doi.org/10.1016/j.xpro.2021.101037
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