Cargando…

Cellular models of pain: New technologies and their potential to progress preclinical research

In vitro models fill a vital niche in preclinical pain research, allowing detailed study of molecular pathways, and in the case of humanised systems, providing a translational bridge between in vivo animal models and human patients. Significant advances in cellular technology available to basic pain...

Descripción completa

Detalles Bibliográficos
Autores principales: Chrysostomidou, Lina, Cooper, Andrew H., Weir, Greg A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683679/
https://www.ncbi.nlm.nih.gov/pubmed/34977426
http://dx.doi.org/10.1016/j.ynpai.2021.100063
_version_ 1784617468674703360
author Chrysostomidou, Lina
Cooper, Andrew H.
Weir, Greg A.
author_facet Chrysostomidou, Lina
Cooper, Andrew H.
Weir, Greg A.
author_sort Chrysostomidou, Lina
collection PubMed
description In vitro models fill a vital niche in preclinical pain research, allowing detailed study of molecular pathways, and in the case of humanised systems, providing a translational bridge between in vivo animal models and human patients. Significant advances in cellular technology available to basic pain researchers have occurred in the last decade, including developing protocols to differentiate sensory neuron-like cells from stem cells and greater access to human dorsal root ganglion tissue. In this review, we discuss the use of both models in preclinical pain research: What can a human sensory neuron in a dish tell us that rodent in vivo models cannot? How similar are these models to their endogenous counterparts, and how should we judge them? What limitations do we need to consider? How can we leverage cell models to improve translational success? In vitro human sensory neuron models equip pain researchers with a valuable tool to investigate human nociception. With continual development, consideration for their advantages and limitations, and effective integration with other experimental strategies, they could become a driving force for the pain field's advancement.
format Online
Article
Text
id pubmed-8683679
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-86836792021-12-30 Cellular models of pain: New technologies and their potential to progress preclinical research Chrysostomidou, Lina Cooper, Andrew H. Weir, Greg A. Neurobiol Pain Mini Review In vitro models fill a vital niche in preclinical pain research, allowing detailed study of molecular pathways, and in the case of humanised systems, providing a translational bridge between in vivo animal models and human patients. Significant advances in cellular technology available to basic pain researchers have occurred in the last decade, including developing protocols to differentiate sensory neuron-like cells from stem cells and greater access to human dorsal root ganglion tissue. In this review, we discuss the use of both models in preclinical pain research: What can a human sensory neuron in a dish tell us that rodent in vivo models cannot? How similar are these models to their endogenous counterparts, and how should we judge them? What limitations do we need to consider? How can we leverage cell models to improve translational success? In vitro human sensory neuron models equip pain researchers with a valuable tool to investigate human nociception. With continual development, consideration for their advantages and limitations, and effective integration with other experimental strategies, they could become a driving force for the pain field's advancement. Elsevier 2021-05-21 /pmc/articles/PMC8683679/ /pubmed/34977426 http://dx.doi.org/10.1016/j.ynpai.2021.100063 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Mini Review
Chrysostomidou, Lina
Cooper, Andrew H.
Weir, Greg A.
Cellular models of pain: New technologies and their potential to progress preclinical research
title Cellular models of pain: New technologies and their potential to progress preclinical research
title_full Cellular models of pain: New technologies and their potential to progress preclinical research
title_fullStr Cellular models of pain: New technologies and their potential to progress preclinical research
title_full_unstemmed Cellular models of pain: New technologies and their potential to progress preclinical research
title_short Cellular models of pain: New technologies and their potential to progress preclinical research
title_sort cellular models of pain: new technologies and their potential to progress preclinical research
topic Mini Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683679/
https://www.ncbi.nlm.nih.gov/pubmed/34977426
http://dx.doi.org/10.1016/j.ynpai.2021.100063
work_keys_str_mv AT chrysostomidoulina cellularmodelsofpainnewtechnologiesandtheirpotentialtoprogresspreclinicalresearch
AT cooperandrewh cellularmodelsofpainnewtechnologiesandtheirpotentialtoprogresspreclinicalresearch
AT weirgrega cellularmodelsofpainnewtechnologiesandtheirpotentialtoprogresspreclinicalresearch