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Insecticide susceptibility status and knockdown resistance (kdr) mutation in Aedes albopictus in China
BACKGROUND: Aedes (Stegomyia) albopictus (Skuse, 1894) is the main vector of dengue virus in China. The resistance to insecticides is a huge obstacle for the control of this species, and determining its resistance status and mechanisms in China is essential for the implementation of vector managemen...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684111/ https://www.ncbi.nlm.nih.gov/pubmed/34922622 http://dx.doi.org/10.1186/s13071-021-05095-5 |
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author | Wei, Yong Zheng, Xueli He, Song Xin, Xuli Zhang, Jiachun Hu, Ke Zhou, Guofa Zhong, Daibin |
author_facet | Wei, Yong Zheng, Xueli He, Song Xin, Xuli Zhang, Jiachun Hu, Ke Zhou, Guofa Zhong, Daibin |
author_sort | Wei, Yong |
collection | PubMed |
description | BACKGROUND: Aedes (Stegomyia) albopictus (Skuse, 1894) is the main vector of dengue virus in China. The resistance to insecticides is a huge obstacle for the control of this species, and determining its resistance status and mechanisms in China is essential for the implementation of vector management strategies. METHODS: We have investigated the larval and adult resistance status of Ae. albopictus to deltamethrin in eight field populations in China. Mutations at the voltage-gated sodium channel gene, related to the knockdown resistance (kdr) effect, were detected by sequencing of PCR products. The eight field populations were examined for pyrethroid resistance using the World Health Organization standard bioassays, and the association between the mutations and phenotypic resistance was tested. RESULTS: The eight field populations of larvae of Ae. albopictus in China exhibited high resistance to deltamethrin; the RR(50) values ranged from 12 (ZJ) to 44 (GZ). Adult bioassay revealed that Ae. albopictus populations were resistant to deltamethrin (mortality rate < 90%), except ZJ population (probably resistant, mortality rate = 93.5%). Long knockdown time in the field populations was consistent with low mortality rates in adult bioassay. F1534S mutation showed increased protection against deltamethrin in all populations except BJ and SJZ populations, whereas I1532T mutation showed increased protection against deltamethrin in only BJ population. CONCLUSION: There were different degrees of resistance to deltamethrin in field Ae. albopictus populations in China. The longest knockdown time and lowest mortality rate observed in Ae. albopictus population in Guangzhou indicate the severity of high resistance to deltamethrin. The patchy distribution of deltamethrin resistance and kdr mutations in Ae. albopictus mosquitoes suggests the necessity for resistance management and developing counter measures to mitigate the spread of resistance. GRAPHICAL ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-8684111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-86841112021-12-20 Insecticide susceptibility status and knockdown resistance (kdr) mutation in Aedes albopictus in China Wei, Yong Zheng, Xueli He, Song Xin, Xuli Zhang, Jiachun Hu, Ke Zhou, Guofa Zhong, Daibin Parasit Vectors Research BACKGROUND: Aedes (Stegomyia) albopictus (Skuse, 1894) is the main vector of dengue virus in China. The resistance to insecticides is a huge obstacle for the control of this species, and determining its resistance status and mechanisms in China is essential for the implementation of vector management strategies. METHODS: We have investigated the larval and adult resistance status of Ae. albopictus to deltamethrin in eight field populations in China. Mutations at the voltage-gated sodium channel gene, related to the knockdown resistance (kdr) effect, were detected by sequencing of PCR products. The eight field populations were examined for pyrethroid resistance using the World Health Organization standard bioassays, and the association between the mutations and phenotypic resistance was tested. RESULTS: The eight field populations of larvae of Ae. albopictus in China exhibited high resistance to deltamethrin; the RR(50) values ranged from 12 (ZJ) to 44 (GZ). Adult bioassay revealed that Ae. albopictus populations were resistant to deltamethrin (mortality rate < 90%), except ZJ population (probably resistant, mortality rate = 93.5%). Long knockdown time in the field populations was consistent with low mortality rates in adult bioassay. F1534S mutation showed increased protection against deltamethrin in all populations except BJ and SJZ populations, whereas I1532T mutation showed increased protection against deltamethrin in only BJ population. CONCLUSION: There were different degrees of resistance to deltamethrin in field Ae. albopictus populations in China. The longest knockdown time and lowest mortality rate observed in Ae. albopictus population in Guangzhou indicate the severity of high resistance to deltamethrin. The patchy distribution of deltamethrin resistance and kdr mutations in Ae. albopictus mosquitoes suggests the necessity for resistance management and developing counter measures to mitigate the spread of resistance. GRAPHICAL ABSTRACT: [Image: see text] BioMed Central 2021-12-18 /pmc/articles/PMC8684111/ /pubmed/34922622 http://dx.doi.org/10.1186/s13071-021-05095-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Wei, Yong Zheng, Xueli He, Song Xin, Xuli Zhang, Jiachun Hu, Ke Zhou, Guofa Zhong, Daibin Insecticide susceptibility status and knockdown resistance (kdr) mutation in Aedes albopictus in China |
title | Insecticide susceptibility status and knockdown resistance (kdr) mutation in Aedes albopictus in China |
title_full | Insecticide susceptibility status and knockdown resistance (kdr) mutation in Aedes albopictus in China |
title_fullStr | Insecticide susceptibility status and knockdown resistance (kdr) mutation in Aedes albopictus in China |
title_full_unstemmed | Insecticide susceptibility status and knockdown resistance (kdr) mutation in Aedes albopictus in China |
title_short | Insecticide susceptibility status and knockdown resistance (kdr) mutation in Aedes albopictus in China |
title_sort | insecticide susceptibility status and knockdown resistance (kdr) mutation in aedes albopictus in china |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684111/ https://www.ncbi.nlm.nih.gov/pubmed/34922622 http://dx.doi.org/10.1186/s13071-021-05095-5 |
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