Cargando…

Microbial associations of shallow-water Mediterranean marine cave Solenogastres (Mollusca)

The first cave-dwelling Solenogastres—marine shell-less worm-like mollusks—were sampled from Mediterranean marine caves floor silt in the Marseille area. The mollusks were 1.5 mm in length, had a transparent body with shiny spicules and appear to represent a new Tegulaherpia species. Electron micros...

Descripción completa

Detalles Bibliográficos
Autores principales: Vortsepneva, Elena, Chevaldonné, Pierre, Klyukina, Alexandra, Naduvaeva, Elizaveta, Todt, Christiane, Zhadan, Anna, Tzetlin, Alexander, Kublanov, Ilya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684320/
https://www.ncbi.nlm.nih.gov/pubmed/35003936
http://dx.doi.org/10.7717/peerj.12655
_version_ 1784617595891089408
author Vortsepneva, Elena
Chevaldonné, Pierre
Klyukina, Alexandra
Naduvaeva, Elizaveta
Todt, Christiane
Zhadan, Anna
Tzetlin, Alexander
Kublanov, Ilya
author_facet Vortsepneva, Elena
Chevaldonné, Pierre
Klyukina, Alexandra
Naduvaeva, Elizaveta
Todt, Christiane
Zhadan, Anna
Tzetlin, Alexander
Kublanov, Ilya
author_sort Vortsepneva, Elena
collection PubMed
description The first cave-dwelling Solenogastres—marine shell-less worm-like mollusks—were sampled from Mediterranean marine caves floor silt in the Marseille area. The mollusks were 1.5 mm in length, had a transparent body with shiny spicules and appear to represent a new Tegulaherpia species. Electron microscopy revealed a high number of microbial cells, located on the surface of the spicules as well as in the cuticle of Tegulaherpia sp. The observed microbial cells varied in morphology and were unequally distributed through the cuticle, reaching a highest density on the dorsal and lateral sides and being practically absent on the ventral side. Next Generation Sequencing (NGS) of V4 region of 16S rRNA gene amplicons, obtained from the DNA samples of whole bodies of Tegulaherpia sp. revealed three dominating microorganisms, two of which were bacteria of Bacteroidetes and Nitrospirae phyla, while the third one represented archaea of Thaumarchaeota phylum. The Operational Taxonomic Unit (OTU), affiliated with Bacteroidetes was an uncultured bacteria of the family Saprospiraceae (93–95% of Bacteroidetes and 25–44% of the total community, depending on sample), OTU, affiliated with Nitrospirae belonged to the genus Nitrospira (8–30% of the community), while the thaumarchaeal OTU was classified as Candidatus Nitrosopumilus (11–15% of the community). Members of these three microbial taxa are known to form associations with various marine animals such as sponges or snails where they contribute to nitrogen metabolism or the decomposition of biopolymers. A similar role is assumed to be played by the microorganisms associated with Tegulaherpia sp.
format Online
Article
Text
id pubmed-8684320
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-86843202022-01-06 Microbial associations of shallow-water Mediterranean marine cave Solenogastres (Mollusca) Vortsepneva, Elena Chevaldonné, Pierre Klyukina, Alexandra Naduvaeva, Elizaveta Todt, Christiane Zhadan, Anna Tzetlin, Alexander Kublanov, Ilya PeerJ Marine Biology The first cave-dwelling Solenogastres—marine shell-less worm-like mollusks—were sampled from Mediterranean marine caves floor silt in the Marseille area. The mollusks were 1.5 mm in length, had a transparent body with shiny spicules and appear to represent a new Tegulaherpia species. Electron microscopy revealed a high number of microbial cells, located on the surface of the spicules as well as in the cuticle of Tegulaherpia sp. The observed microbial cells varied in morphology and were unequally distributed through the cuticle, reaching a highest density on the dorsal and lateral sides and being practically absent on the ventral side. Next Generation Sequencing (NGS) of V4 region of 16S rRNA gene amplicons, obtained from the DNA samples of whole bodies of Tegulaherpia sp. revealed three dominating microorganisms, two of which were bacteria of Bacteroidetes and Nitrospirae phyla, while the third one represented archaea of Thaumarchaeota phylum. The Operational Taxonomic Unit (OTU), affiliated with Bacteroidetes was an uncultured bacteria of the family Saprospiraceae (93–95% of Bacteroidetes and 25–44% of the total community, depending on sample), OTU, affiliated with Nitrospirae belonged to the genus Nitrospira (8–30% of the community), while the thaumarchaeal OTU was classified as Candidatus Nitrosopumilus (11–15% of the community). Members of these three microbial taxa are known to form associations with various marine animals such as sponges or snails where they contribute to nitrogen metabolism or the decomposition of biopolymers. A similar role is assumed to be played by the microorganisms associated with Tegulaherpia sp. PeerJ Inc. 2021-12-15 /pmc/articles/PMC8684320/ /pubmed/35003936 http://dx.doi.org/10.7717/peerj.12655 Text en ©2021 Vortsepneva et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Marine Biology
Vortsepneva, Elena
Chevaldonné, Pierre
Klyukina, Alexandra
Naduvaeva, Elizaveta
Todt, Christiane
Zhadan, Anna
Tzetlin, Alexander
Kublanov, Ilya
Microbial associations of shallow-water Mediterranean marine cave Solenogastres (Mollusca)
title Microbial associations of shallow-water Mediterranean marine cave Solenogastres (Mollusca)
title_full Microbial associations of shallow-water Mediterranean marine cave Solenogastres (Mollusca)
title_fullStr Microbial associations of shallow-water Mediterranean marine cave Solenogastres (Mollusca)
title_full_unstemmed Microbial associations of shallow-water Mediterranean marine cave Solenogastres (Mollusca)
title_short Microbial associations of shallow-water Mediterranean marine cave Solenogastres (Mollusca)
title_sort microbial associations of shallow-water mediterranean marine cave solenogastres (mollusca)
topic Marine Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684320/
https://www.ncbi.nlm.nih.gov/pubmed/35003936
http://dx.doi.org/10.7717/peerj.12655
work_keys_str_mv AT vortsepnevaelena microbialassociationsofshallowwatermediterraneanmarinecavesolenogastresmollusca
AT chevaldonnepierre microbialassociationsofshallowwatermediterraneanmarinecavesolenogastresmollusca
AT klyukinaalexandra microbialassociationsofshallowwatermediterraneanmarinecavesolenogastresmollusca
AT naduvaevaelizaveta microbialassociationsofshallowwatermediterraneanmarinecavesolenogastresmollusca
AT todtchristiane microbialassociationsofshallowwatermediterraneanmarinecavesolenogastresmollusca
AT zhadananna microbialassociationsofshallowwatermediterraneanmarinecavesolenogastresmollusca
AT tzetlinalexander microbialassociationsofshallowwatermediterraneanmarinecavesolenogastresmollusca
AT kublanovilya microbialassociationsofshallowwatermediterraneanmarinecavesolenogastresmollusca