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Analysis of blood group antigens on MUC5AC in mucinous ovarian cancer tissues using in situ proximity ligation assay

MUC5AC has been indicated to be a marker for mucinous ovarian cancer (OC). We investigated the use of in situ proximity ligation assay (PLA) for blood group ABH expressing MUC5AC to differentiate between serous and mucinous OC, to validate preceding observations that also MUC5AC ABH expression is in...

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Autores principales: Mateoiu, Constantina, Vitiazeva, Varvara, Kristjansdottir, Björg, Weijdegård, Birgitta, Örnros, Jessica, Gallini, Radiosa, Kamali-Moghaddam, Masood, Sundfeldt, Karin, Karlsson, Niclas G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684467/
https://www.ncbi.nlm.nih.gov/pubmed/34459484
http://dx.doi.org/10.1093/glycob/cwab090
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author Mateoiu, Constantina
Vitiazeva, Varvara
Kristjansdottir, Björg
Weijdegård, Birgitta
Örnros, Jessica
Gallini, Radiosa
Kamali-Moghaddam, Masood
Sundfeldt, Karin
Karlsson, Niclas G
author_facet Mateoiu, Constantina
Vitiazeva, Varvara
Kristjansdottir, Björg
Weijdegård, Birgitta
Örnros, Jessica
Gallini, Radiosa
Kamali-Moghaddam, Masood
Sundfeldt, Karin
Karlsson, Niclas G
author_sort Mateoiu, Constantina
collection PubMed
description MUC5AC has been indicated to be a marker for mucinous ovarian cancer (OC). We investigated the use of in situ proximity ligation assay (PLA) for blood group ABH expressing MUC5AC to differentiate between serous and mucinous OC, to validate preceding observations that also MUC5AC ABH expression is increased in mucinous OC. We developed PLA for anti-A, B, and H/anti-MUC5AC and a PLA using a combined lectin Ulex europaeus agglutinin I (UEA I)/anti-MUC5AC assay. The PLAs were verified with mass spectrometry, where mucinous OC secretor positive patients’ cysts fluids containing ABH O-linked oligosaccharides also showed positive OC tissue PLA staining. A nonsecretor mucinous OC cyst fluid was negative for ABH and displayed negative PLA staining of the matched tissue. Using the UEA I/MUC5AC PLA, we screened a tissue micro array of 410 ovarian tissue samples from patients with various stages of mucinous or serous OC, 32 samples with metastasis to the ovaries and 34 controls. The PLA allowed differentiating mucinous tumors with a sensitivity of 84% and a specificity of 97% both against serous cancer but also compared to tissues from controls. This sensitivity is close to the expected incidence of secretor individuals in a population. The recorded sensitivity was also found to be higher compared to mucinous type cancer with metastasis to the ovaries, where only 32% were positive. We conclude that UEA 1/MUC5AC PLA allows glycospecific differentiation between serous and mucinous OC in patients with positive secretor status and will not identify secretor negative individuals with mucinous OC.
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spelling pubmed-86844672021-12-20 Analysis of blood group antigens on MUC5AC in mucinous ovarian cancer tissues using in situ proximity ligation assay Mateoiu, Constantina Vitiazeva, Varvara Kristjansdottir, Björg Weijdegård, Birgitta Örnros, Jessica Gallini, Radiosa Kamali-Moghaddam, Masood Sundfeldt, Karin Karlsson, Niclas G Glycobiology Cancer Biology MUC5AC has been indicated to be a marker for mucinous ovarian cancer (OC). We investigated the use of in situ proximity ligation assay (PLA) for blood group ABH expressing MUC5AC to differentiate between serous and mucinous OC, to validate preceding observations that also MUC5AC ABH expression is increased in mucinous OC. We developed PLA for anti-A, B, and H/anti-MUC5AC and a PLA using a combined lectin Ulex europaeus agglutinin I (UEA I)/anti-MUC5AC assay. The PLAs were verified with mass spectrometry, where mucinous OC secretor positive patients’ cysts fluids containing ABH O-linked oligosaccharides also showed positive OC tissue PLA staining. A nonsecretor mucinous OC cyst fluid was negative for ABH and displayed negative PLA staining of the matched tissue. Using the UEA I/MUC5AC PLA, we screened a tissue micro array of 410 ovarian tissue samples from patients with various stages of mucinous or serous OC, 32 samples with metastasis to the ovaries and 34 controls. The PLA allowed differentiating mucinous tumors with a sensitivity of 84% and a specificity of 97% both against serous cancer but also compared to tissues from controls. This sensitivity is close to the expected incidence of secretor individuals in a population. The recorded sensitivity was also found to be higher compared to mucinous type cancer with metastasis to the ovaries, where only 32% were positive. We conclude that UEA 1/MUC5AC PLA allows glycospecific differentiation between serous and mucinous OC in patients with positive secretor status and will not identify secretor negative individuals with mucinous OC. Oxford University Press 2021-08-23 /pmc/articles/PMC8684467/ /pubmed/34459484 http://dx.doi.org/10.1093/glycob/cwab090 Text en © The Author(s) 2021. Published by Oxford University Press. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Cancer Biology
Mateoiu, Constantina
Vitiazeva, Varvara
Kristjansdottir, Björg
Weijdegård, Birgitta
Örnros, Jessica
Gallini, Radiosa
Kamali-Moghaddam, Masood
Sundfeldt, Karin
Karlsson, Niclas G
Analysis of blood group antigens on MUC5AC in mucinous ovarian cancer tissues using in situ proximity ligation assay
title Analysis of blood group antigens on MUC5AC in mucinous ovarian cancer tissues using in situ proximity ligation assay
title_full Analysis of blood group antigens on MUC5AC in mucinous ovarian cancer tissues using in situ proximity ligation assay
title_fullStr Analysis of blood group antigens on MUC5AC in mucinous ovarian cancer tissues using in situ proximity ligation assay
title_full_unstemmed Analysis of blood group antigens on MUC5AC in mucinous ovarian cancer tissues using in situ proximity ligation assay
title_short Analysis of blood group antigens on MUC5AC in mucinous ovarian cancer tissues using in situ proximity ligation assay
title_sort analysis of blood group antigens on muc5ac in mucinous ovarian cancer tissues using in situ proximity ligation assay
topic Cancer Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684467/
https://www.ncbi.nlm.nih.gov/pubmed/34459484
http://dx.doi.org/10.1093/glycob/cwab090
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