Cargando…
Feasibility of Using H(3)PO(4)/H(2)O(2) in the Synthesis of Antimicrobial TiO(2) Nanoporous Surfaces
Ti6Al4V alloys are the primary materials used for clinical bone regeneration and restoration; however, they are substantially susceptible to biomaterial-related infections. Therefore, in the present work, we applied a controllable and stable oxidative nanopatterning strategy by applying H(3)PO(4), a...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684504/ https://www.ncbi.nlm.nih.gov/pubmed/34931122 http://dx.doi.org/10.1155/2021/6209094 |
_version_ | 1784617632855490560 |
---|---|
author | Valdez-Salas, Benjamín Beltrán-Partida, Ernesto |
author_facet | Valdez-Salas, Benjamín Beltrán-Partida, Ernesto |
author_sort | Valdez-Salas, Benjamín |
collection | PubMed |
description | Ti6Al4V alloys are the primary materials used for clinical bone regeneration and restoration; however, they are substantially susceptible to biomaterial-related infections. Therefore, in the present work, we applied a controllable and stable oxidative nanopatterning strategy by applying H(3)PO(4), a weaker dissociating acid, as a substitute for H(2)SO(4) in the classical piranha reaction. The results suggest that our method acted as a concomitant platform to develop reproducible diameter-controlled TiO(2) nanopores (NPs). Interestingly, our procedure illustrated stable temperature reactions without exothermic responses since the addition of mixture preparation to the nanopatterning reactions. The reactions were carried out for 30 min (NP14), 1 h (NP7), and 2 h (NP36), suggesting the formation of a thin nanopore layer as observed by Raman spectroscopy. Moreover, the antimicrobial activity revealed that NP7 could disrupt active microbial colonization for 2 h and 6 h. The phenotype configuration strikingly showed that NP7 does not alter the cell morphology, thus proposing a disruptive adhesion pathway instead of cellular lysis. Furthermore, preliminary assays suggested an early promoted osteoblasts viability in comparison to the control material. Our work opens a new path for the rationale design of nanobiomaterials with “intelligent surfaces” capable of decreasing microbial adhesion, increasing osteoblast viability, and being scalable for industrial transfer. |
format | Online Article Text |
id | pubmed-8684504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-86845042021-12-19 Feasibility of Using H(3)PO(4)/H(2)O(2) in the Synthesis of Antimicrobial TiO(2) Nanoporous Surfaces Valdez-Salas, Benjamín Beltrán-Partida, Ernesto Bioinorg Chem Appl Research Article Ti6Al4V alloys are the primary materials used for clinical bone regeneration and restoration; however, they are substantially susceptible to biomaterial-related infections. Therefore, in the present work, we applied a controllable and stable oxidative nanopatterning strategy by applying H(3)PO(4), a weaker dissociating acid, as a substitute for H(2)SO(4) in the classical piranha reaction. The results suggest that our method acted as a concomitant platform to develop reproducible diameter-controlled TiO(2) nanopores (NPs). Interestingly, our procedure illustrated stable temperature reactions without exothermic responses since the addition of mixture preparation to the nanopatterning reactions. The reactions were carried out for 30 min (NP14), 1 h (NP7), and 2 h (NP36), suggesting the formation of a thin nanopore layer as observed by Raman spectroscopy. Moreover, the antimicrobial activity revealed that NP7 could disrupt active microbial colonization for 2 h and 6 h. The phenotype configuration strikingly showed that NP7 does not alter the cell morphology, thus proposing a disruptive adhesion pathway instead of cellular lysis. Furthermore, preliminary assays suggested an early promoted osteoblasts viability in comparison to the control material. Our work opens a new path for the rationale design of nanobiomaterials with “intelligent surfaces” capable of decreasing microbial adhesion, increasing osteoblast viability, and being scalable for industrial transfer. Hindawi 2021-12-11 /pmc/articles/PMC8684504/ /pubmed/34931122 http://dx.doi.org/10.1155/2021/6209094 Text en Copyright © 2021 Benjamín Valdez-Salas and Ernesto Beltrán-Partida. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Valdez-Salas, Benjamín Beltrán-Partida, Ernesto Feasibility of Using H(3)PO(4)/H(2)O(2) in the Synthesis of Antimicrobial TiO(2) Nanoporous Surfaces |
title | Feasibility of Using H(3)PO(4)/H(2)O(2) in the Synthesis of Antimicrobial TiO(2) Nanoporous Surfaces |
title_full | Feasibility of Using H(3)PO(4)/H(2)O(2) in the Synthesis of Antimicrobial TiO(2) Nanoporous Surfaces |
title_fullStr | Feasibility of Using H(3)PO(4)/H(2)O(2) in the Synthesis of Antimicrobial TiO(2) Nanoporous Surfaces |
title_full_unstemmed | Feasibility of Using H(3)PO(4)/H(2)O(2) in the Synthesis of Antimicrobial TiO(2) Nanoporous Surfaces |
title_short | Feasibility of Using H(3)PO(4)/H(2)O(2) in the Synthesis of Antimicrobial TiO(2) Nanoporous Surfaces |
title_sort | feasibility of using h(3)po(4)/h(2)o(2) in the synthesis of antimicrobial tio(2) nanoporous surfaces |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684504/ https://www.ncbi.nlm.nih.gov/pubmed/34931122 http://dx.doi.org/10.1155/2021/6209094 |
work_keys_str_mv | AT valdezsalasbenjamin feasibilityofusingh3po4h2o2inthesynthesisofantimicrobialtio2nanoporoussurfaces AT beltranpartidaernesto feasibilityofusingh3po4h2o2inthesynthesisofantimicrobialtio2nanoporoussurfaces |