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Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer's Disease

OBJECTIVES: This study compares glycoproteomes in Thai Alzheimer's disease (AD) patients with those of cognitively normal individuals. METHODS: Study participants included outpatients with clinically diagnosed AD (N = 136) and healthy controls without cognitive impairment (N = 183). Blood sampl...

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Autores principales: Kerdsaeng, Naphatthakarn, Roytrakul, Sittiruk, Chanprasertyothin, Suwannee, Charernwat, Piangporn, Chansirikarnjana, Sirintorn, Sritara, Piyamitr, Sirivarasai, Jintana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684523/
https://www.ncbi.nlm.nih.gov/pubmed/34931130
http://dx.doi.org/10.1155/2021/1434076
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author Kerdsaeng, Naphatthakarn
Roytrakul, Sittiruk
Chanprasertyothin, Suwannee
Charernwat, Piangporn
Chansirikarnjana, Sirintorn
Sritara, Piyamitr
Sirivarasai, Jintana
author_facet Kerdsaeng, Naphatthakarn
Roytrakul, Sittiruk
Chanprasertyothin, Suwannee
Charernwat, Piangporn
Chansirikarnjana, Sirintorn
Sritara, Piyamitr
Sirivarasai, Jintana
author_sort Kerdsaeng, Naphatthakarn
collection PubMed
description OBJECTIVES: This study compares glycoproteomes in Thai Alzheimer's disease (AD) patients with those of cognitively normal individuals. METHODS: Study participants included outpatients with clinically diagnosed AD (N = 136) and healthy controls without cognitive impairment (N = 183). Blood samples were collected from all participants for biochemical analysis and for Apolipoprotein E (APOE) genotyping by real-time TaqMan PCR assays. Comparative serum glycoproteomic profiling by liquid chromatography-tandem mass spectrometry was then performed to identify differentially abundant proteins with functional relevance. RESULTS: Statistical differences in age, educational level, and APOE ɛ3/ɛ4 and ɛ4/ɛ4 haplotype frequencies were found between the AD and control groups. The frequency of the APOE ɛ4 allele was significantly higher in the AD group than in the control group. In total, 871 glycoproteins were identified, including 266 and 259 unique proteins in control and AD groups, respectively. There were 49 and 297 upregulated and downregulated glycoproteins, respectively, in AD samples compared with the controls. Unique AD glycoproteins were associated with numerous pathways, including Alzheimer's disease-presenilin pathway (16.6%), inflammation pathway mediated by chemokine and cytokine signaling (9.2%), Wnt signaling pathway (8.2%), and apoptosis signaling pathway (6.7%). CONCLUSION: Functions and pathways associated with protein-protein interactions were identified in AD. Significant changes in these proteins can indicate the molecular mechanisms involved in the pathogenesis of AD, and they have the potential to serve as AD biomarkers. Such findings could allow us to better understand AD pathology.
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spelling pubmed-86845232021-12-19 Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer's Disease Kerdsaeng, Naphatthakarn Roytrakul, Sittiruk Chanprasertyothin, Suwannee Charernwat, Piangporn Chansirikarnjana, Sirintorn Sritara, Piyamitr Sirivarasai, Jintana Behav Neurol Research Article OBJECTIVES: This study compares glycoproteomes in Thai Alzheimer's disease (AD) patients with those of cognitively normal individuals. METHODS: Study participants included outpatients with clinically diagnosed AD (N = 136) and healthy controls without cognitive impairment (N = 183). Blood samples were collected from all participants for biochemical analysis and for Apolipoprotein E (APOE) genotyping by real-time TaqMan PCR assays. Comparative serum glycoproteomic profiling by liquid chromatography-tandem mass spectrometry was then performed to identify differentially abundant proteins with functional relevance. RESULTS: Statistical differences in age, educational level, and APOE ɛ3/ɛ4 and ɛ4/ɛ4 haplotype frequencies were found between the AD and control groups. The frequency of the APOE ɛ4 allele was significantly higher in the AD group than in the control group. In total, 871 glycoproteins were identified, including 266 and 259 unique proteins in control and AD groups, respectively. There were 49 and 297 upregulated and downregulated glycoproteins, respectively, in AD samples compared with the controls. Unique AD glycoproteins were associated with numerous pathways, including Alzheimer's disease-presenilin pathway (16.6%), inflammation pathway mediated by chemokine and cytokine signaling (9.2%), Wnt signaling pathway (8.2%), and apoptosis signaling pathway (6.7%). CONCLUSION: Functions and pathways associated with protein-protein interactions were identified in AD. Significant changes in these proteins can indicate the molecular mechanisms involved in the pathogenesis of AD, and they have the potential to serve as AD biomarkers. Such findings could allow us to better understand AD pathology. Hindawi 2021-12-11 /pmc/articles/PMC8684523/ /pubmed/34931130 http://dx.doi.org/10.1155/2021/1434076 Text en Copyright © 2021 Naphatthakarn Kerdsaeng et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kerdsaeng, Naphatthakarn
Roytrakul, Sittiruk
Chanprasertyothin, Suwannee
Charernwat, Piangporn
Chansirikarnjana, Sirintorn
Sritara, Piyamitr
Sirivarasai, Jintana
Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer's Disease
title Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer's Disease
title_full Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer's Disease
title_fullStr Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer's Disease
title_full_unstemmed Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer's Disease
title_short Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer's Disease
title_sort serum glycoproteomics and identification of potential mechanisms underlying alzheimer's disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684523/
https://www.ncbi.nlm.nih.gov/pubmed/34931130
http://dx.doi.org/10.1155/2021/1434076
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