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Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain
Streptococcus suis is a zoonotic pathogen of swine involved in arthritis, polyserositis, and meningitis. Colonization of piglets by S. suis is very common and occurs early in life. The clinical outcome of infection is influenced by the virulence of the S. suis strains and the immunity of the animals...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684544/ https://www.ncbi.nlm.nih.gov/pubmed/34924012 http://dx.doi.org/10.1186/s13567-021-01013-w |
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author | Neila-Ibáñez, Carlos Brogaard, Louise Pailler-García, Lola Martínez, Jorge Segalés, Joaquim Segura, Mariela Heegaard, Peter M. H. Aragon, Virginia |
author_facet | Neila-Ibáñez, Carlos Brogaard, Louise Pailler-García, Lola Martínez, Jorge Segalés, Joaquim Segura, Mariela Heegaard, Peter M. H. Aragon, Virginia |
author_sort | Neila-Ibáñez, Carlos |
collection | PubMed |
description | Streptococcus suis is a zoonotic pathogen of swine involved in arthritis, polyserositis, and meningitis. Colonization of piglets by S. suis is very common and occurs early in life. The clinical outcome of infection is influenced by the virulence of the S. suis strains and the immunity of the animals. Here, the role of innate immunity was studied in cesarean-derived colostrum-deprived piglets inoculated intranasally with either virulent S. suis strain 10 (S10) or non-virulent S. suis strain T15. Colonization of the inoculated piglets was confirmed at the end of the study by PCR and immunohistochemistry. Fever (≥40.5 °C) was more prevalent in piglets inoculated with S10 compared to T15 at 4 h after inoculation. During the 3 days of monitoring, no other major clinical signs were detected. Accordingly, only small changes in transcription of genes associated with the antibacterial innate immune response were observed at systemic sites, with S10 inducing an earlier response than T15 in blood. Local inflammatory response to the inoculation, evaluated by transcriptional analysis of selected genes in nasal swabs, was more sustained in piglets inoculated with the virulent S10, as demonstrated by transcription of inflammation-related genes, such as IL1B, IL1A, and IRF7. In contrast, most of the gene expression changes in trachea, lungs, and associated lymph nodes were observed in response to the non-virulent T15 strain. Thus, S. suis colonization in the absence of systemic infection induces an innate immune response in piglets that appears to be related to the virulence potential of the colonizing strain. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-021-01013-w. |
format | Online Article Text |
id | pubmed-8684544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-86845442021-12-20 Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain Neila-Ibáñez, Carlos Brogaard, Louise Pailler-García, Lola Martínez, Jorge Segalés, Joaquim Segura, Mariela Heegaard, Peter M. H. Aragon, Virginia Vet Res Research Article Streptococcus suis is a zoonotic pathogen of swine involved in arthritis, polyserositis, and meningitis. Colonization of piglets by S. suis is very common and occurs early in life. The clinical outcome of infection is influenced by the virulence of the S. suis strains and the immunity of the animals. Here, the role of innate immunity was studied in cesarean-derived colostrum-deprived piglets inoculated intranasally with either virulent S. suis strain 10 (S10) or non-virulent S. suis strain T15. Colonization of the inoculated piglets was confirmed at the end of the study by PCR and immunohistochemistry. Fever (≥40.5 °C) was more prevalent in piglets inoculated with S10 compared to T15 at 4 h after inoculation. During the 3 days of monitoring, no other major clinical signs were detected. Accordingly, only small changes in transcription of genes associated with the antibacterial innate immune response were observed at systemic sites, with S10 inducing an earlier response than T15 in blood. Local inflammatory response to the inoculation, evaluated by transcriptional analysis of selected genes in nasal swabs, was more sustained in piglets inoculated with the virulent S10, as demonstrated by transcription of inflammation-related genes, such as IL1B, IL1A, and IRF7. In contrast, most of the gene expression changes in trachea, lungs, and associated lymph nodes were observed in response to the non-virulent T15 strain. Thus, S. suis colonization in the absence of systemic infection induces an innate immune response in piglets that appears to be related to the virulence potential of the colonizing strain. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-021-01013-w. BioMed Central 2021-12-19 2021 /pmc/articles/PMC8684544/ /pubmed/34924012 http://dx.doi.org/10.1186/s13567-021-01013-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Neila-Ibáñez, Carlos Brogaard, Louise Pailler-García, Lola Martínez, Jorge Segalés, Joaquim Segura, Mariela Heegaard, Peter M. H. Aragon, Virginia Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
title | Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
title_full | Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
title_fullStr | Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
title_full_unstemmed | Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
title_short | Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
title_sort | piglet innate immune response to streptococcus suis colonization is modulated by the virulence of the strain |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684544/ https://www.ncbi.nlm.nih.gov/pubmed/34924012 http://dx.doi.org/10.1186/s13567-021-01013-w |
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