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Skin substitutes with noncultured autologous skin cell suspension heal porcine full‐thickness wounds in a one‐stage procedure

Clinical application of skin substitute is typically a two‐stage procedure with application of skin substitute matrix to the wound followed by engraftment of a split‐thickness skin graft (STSG). This two‐stage procedure requires multiple interventions, increasing the time until the wound is epitheli...

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Autores principales: Damaraju, Sita M., Mintz, Benjamin R., Park, J. Genevieve, Gandhi, Ankur, Saini, Sunil, Molnar, Joseph A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684857/
https://www.ncbi.nlm.nih.gov/pubmed/34036743
http://dx.doi.org/10.1111/iwj.13615
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author Damaraju, Sita M.
Mintz, Benjamin R.
Park, J. Genevieve
Gandhi, Ankur
Saini, Sunil
Molnar, Joseph A.
author_facet Damaraju, Sita M.
Mintz, Benjamin R.
Park, J. Genevieve
Gandhi, Ankur
Saini, Sunil
Molnar, Joseph A.
author_sort Damaraju, Sita M.
collection PubMed
description Clinical application of skin substitute is typically a two‐stage procedure with application of skin substitute matrix to the wound followed by engraftment of a split‐thickness skin graft (STSG). This two‐stage procedure requires multiple interventions, increasing the time until the wound is epithelialised. In this study, the feasibility of a one‐stage procedure by combining bioengineered collagen‐chondroitin‐6‐sulfate (DS1) or decellularised fetal bovine skin substitute (DS2) with autologous skin cell suspension (ASCS) in a porcine full‐thickness wound healing model was evaluated. Twelve full‐thickness excisional wounds on the backs of pigs received one of six different treatments: empty; ASCS; DS1 with or without ASCS; DS2 with or without ASCS. The ASCS was prepared using a point‐of‐care device and was seeded onto the bottom side of DS1, DS2, and empty wounds at 80 000 cells/cm(2). Wound measurements and photographs were taken on days 0, 9, 14, 21, 28, 35, and 42 post‐wounding. Histological analysis was performed on samples obtained on days 9, 14, 28, and 42. Wounds in the empty group or with ASCS alone showed increased wound contraction, fibrosis, and myofibroblast density compared with other treatment groups. The addition of ASCS to DS1 or DS2 resulted in a marked increase in re‐epithelialisation of wounds at 14 days, from 15 ± 11% to 71 ± 20% (DS1 vs DS1 + ASCS) or 28 ± 14% to 77 ± 26 (DS2 vs DS2 + ASCS) despite different mechanisms of tissue regeneration employed by the DS used. These results suggest that this approach may be a viable one‐stage treatment in clinical practice.
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spelling pubmed-86848572021-12-30 Skin substitutes with noncultured autologous skin cell suspension heal porcine full‐thickness wounds in a one‐stage procedure Damaraju, Sita M. Mintz, Benjamin R. Park, J. Genevieve Gandhi, Ankur Saini, Sunil Molnar, Joseph A. Int Wound J Original Articles Clinical application of skin substitute is typically a two‐stage procedure with application of skin substitute matrix to the wound followed by engraftment of a split‐thickness skin graft (STSG). This two‐stage procedure requires multiple interventions, increasing the time until the wound is epithelialised. In this study, the feasibility of a one‐stage procedure by combining bioengineered collagen‐chondroitin‐6‐sulfate (DS1) or decellularised fetal bovine skin substitute (DS2) with autologous skin cell suspension (ASCS) in a porcine full‐thickness wound healing model was evaluated. Twelve full‐thickness excisional wounds on the backs of pigs received one of six different treatments: empty; ASCS; DS1 with or without ASCS; DS2 with or without ASCS. The ASCS was prepared using a point‐of‐care device and was seeded onto the bottom side of DS1, DS2, and empty wounds at 80 000 cells/cm(2). Wound measurements and photographs were taken on days 0, 9, 14, 21, 28, 35, and 42 post‐wounding. Histological analysis was performed on samples obtained on days 9, 14, 28, and 42. Wounds in the empty group or with ASCS alone showed increased wound contraction, fibrosis, and myofibroblast density compared with other treatment groups. The addition of ASCS to DS1 or DS2 resulted in a marked increase in re‐epithelialisation of wounds at 14 days, from 15 ± 11% to 71 ± 20% (DS1 vs DS1 + ASCS) or 28 ± 14% to 77 ± 26 (DS2 vs DS2 + ASCS) despite different mechanisms of tissue regeneration employed by the DS used. These results suggest that this approach may be a viable one‐stage treatment in clinical practice. Blackwell Publishing Ltd 2021-05-25 /pmc/articles/PMC8684857/ /pubmed/34036743 http://dx.doi.org/10.1111/iwj.13615 Text en © 2021 The Authors. International Wound Journal published by Medicalhelplines.com Inc (3M) and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Damaraju, Sita M.
Mintz, Benjamin R.
Park, J. Genevieve
Gandhi, Ankur
Saini, Sunil
Molnar, Joseph A.
Skin substitutes with noncultured autologous skin cell suspension heal porcine full‐thickness wounds in a one‐stage procedure
title Skin substitutes with noncultured autologous skin cell suspension heal porcine full‐thickness wounds in a one‐stage procedure
title_full Skin substitutes with noncultured autologous skin cell suspension heal porcine full‐thickness wounds in a one‐stage procedure
title_fullStr Skin substitutes with noncultured autologous skin cell suspension heal porcine full‐thickness wounds in a one‐stage procedure
title_full_unstemmed Skin substitutes with noncultured autologous skin cell suspension heal porcine full‐thickness wounds in a one‐stage procedure
title_short Skin substitutes with noncultured autologous skin cell suspension heal porcine full‐thickness wounds in a one‐stage procedure
title_sort skin substitutes with noncultured autologous skin cell suspension heal porcine full‐thickness wounds in a one‐stage procedure
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684857/
https://www.ncbi.nlm.nih.gov/pubmed/34036743
http://dx.doi.org/10.1111/iwj.13615
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