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Effect of pH on the Electrochemical Behavior of Hydrogen Peroxide in the Presence of Pseudomonas aeruginosa
The influence of pH on the electrochemical behavior of hydrogen peroxide in the presence of Pseudomonas aeruginosa was investigated using electrochemical techniques. Cyclic and square wave voltammetry were used to monitor the enzymatic activity. A modified cobalt phthalocyanine (CoPc) carbon electro...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685425/ https://www.ncbi.nlm.nih.gov/pubmed/34938720 http://dx.doi.org/10.3389/fbioe.2021.749057 |
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author | Espinoza-Vergara, Javier Molina, Paulo Walter, Mariana Gulppi, Miguel Vejar, Nelson Melo, Francisco Urzua, Marcela Muñoz, Hugo Zagal, José H. Zhou, Xiaorong Azocar, Manuel I. Paez, Maritza A. |
author_facet | Espinoza-Vergara, Javier Molina, Paulo Walter, Mariana Gulppi, Miguel Vejar, Nelson Melo, Francisco Urzua, Marcela Muñoz, Hugo Zagal, José H. Zhou, Xiaorong Azocar, Manuel I. Paez, Maritza A. |
author_sort | Espinoza-Vergara, Javier |
collection | PubMed |
description | The influence of pH on the electrochemical behavior of hydrogen peroxide in the presence of Pseudomonas aeruginosa was investigated using electrochemical techniques. Cyclic and square wave voltammetry were used to monitor the enzymatic activity. A modified cobalt phthalocyanine (CoPc) carbon electrode (OPG), a known catalyst for reducing O(2) to H(2)O(2), was used to detect species resulting from the enzyme activity. The electrolyte was a sterilized aqueous medium containing Mueller-Hinton (MH) broth. The open-circuit potential (OCP) of the Pseudomonas aeruginosa culture in MH decreased rapidly with time, reaching a stable state after 4 h. Peculiarities in the E / I response were observed in voltammograms conducted in less than 4 h of exposure to the culture medium. Such particular E/I responses are due to the catalase’s enzymatic action related to the conversion of hydrogen peroxide to oxygen, confirming the authors’ previous findings related to the behavior of other catalase-positive microorganisms. The enzymatic activity exhibits maximum activity at pH 7.5, assessed by the potential at which oxygen is reduced to hydrogen peroxide. At higher or lower pHs, the oxygen reduction reaction (ORR) occurs at higher overpotentials, i.e., at more negative potentials. In addition, and to assess the influence of bacterial adhesion on the electrochemical behavior, measurements of the bacterial-substrate metal interaction were performed at different pH using atomic force microscopy. |
format | Online Article Text |
id | pubmed-8685425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86854252021-12-21 Effect of pH on the Electrochemical Behavior of Hydrogen Peroxide in the Presence of Pseudomonas aeruginosa Espinoza-Vergara, Javier Molina, Paulo Walter, Mariana Gulppi, Miguel Vejar, Nelson Melo, Francisco Urzua, Marcela Muñoz, Hugo Zagal, José H. Zhou, Xiaorong Azocar, Manuel I. Paez, Maritza A. Front Bioeng Biotechnol Bioengineering and Biotechnology The influence of pH on the electrochemical behavior of hydrogen peroxide in the presence of Pseudomonas aeruginosa was investigated using electrochemical techniques. Cyclic and square wave voltammetry were used to monitor the enzymatic activity. A modified cobalt phthalocyanine (CoPc) carbon electrode (OPG), a known catalyst for reducing O(2) to H(2)O(2), was used to detect species resulting from the enzyme activity. The electrolyte was a sterilized aqueous medium containing Mueller-Hinton (MH) broth. The open-circuit potential (OCP) of the Pseudomonas aeruginosa culture in MH decreased rapidly with time, reaching a stable state after 4 h. Peculiarities in the E / I response were observed in voltammograms conducted in less than 4 h of exposure to the culture medium. Such particular E/I responses are due to the catalase’s enzymatic action related to the conversion of hydrogen peroxide to oxygen, confirming the authors’ previous findings related to the behavior of other catalase-positive microorganisms. The enzymatic activity exhibits maximum activity at pH 7.5, assessed by the potential at which oxygen is reduced to hydrogen peroxide. At higher or lower pHs, the oxygen reduction reaction (ORR) occurs at higher overpotentials, i.e., at more negative potentials. In addition, and to assess the influence of bacterial adhesion on the electrochemical behavior, measurements of the bacterial-substrate metal interaction were performed at different pH using atomic force microscopy. Frontiers Media S.A. 2021-12-06 /pmc/articles/PMC8685425/ /pubmed/34938720 http://dx.doi.org/10.3389/fbioe.2021.749057 Text en Copyright © 2021 Espinoza-Vergara, Molina, Walter, Gulppi, Vejar, Melo, Urzua, Muñoz, Zagal, Zhou, Azocar and Paez. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Espinoza-Vergara, Javier Molina, Paulo Walter, Mariana Gulppi, Miguel Vejar, Nelson Melo, Francisco Urzua, Marcela Muñoz, Hugo Zagal, José H. Zhou, Xiaorong Azocar, Manuel I. Paez, Maritza A. Effect of pH on the Electrochemical Behavior of Hydrogen Peroxide in the Presence of Pseudomonas aeruginosa |
title | Effect of pH on the Electrochemical Behavior of Hydrogen Peroxide in the Presence of Pseudomonas aeruginosa
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title_full | Effect of pH on the Electrochemical Behavior of Hydrogen Peroxide in the Presence of Pseudomonas aeruginosa
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title_fullStr | Effect of pH on the Electrochemical Behavior of Hydrogen Peroxide in the Presence of Pseudomonas aeruginosa
|
title_full_unstemmed | Effect of pH on the Electrochemical Behavior of Hydrogen Peroxide in the Presence of Pseudomonas aeruginosa
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title_short | Effect of pH on the Electrochemical Behavior of Hydrogen Peroxide in the Presence of Pseudomonas aeruginosa
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title_sort | effect of ph on the electrochemical behavior of hydrogen peroxide in the presence of pseudomonas aeruginosa |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685425/ https://www.ncbi.nlm.nih.gov/pubmed/34938720 http://dx.doi.org/10.3389/fbioe.2021.749057 |
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