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S-RNase Alleles Associated With Self-Compatibility in the Tomato Clade: Structure, Origins, and Expression Plasticity

The self-incompatibility (SI) system in the Solanaceae is comprised of cytotoxic pistil S-RNases which are countered by S-locus F-box (SLF) resistance factors found in pollen. Under this barrier-resistance architecture, mating system transitions from SI to self-compatibility (SC) typically result fr...

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Autores principales: Broz, Amanda K., Miller, Christopher M., Baek, You Soon, Tovar-Méndez, Alejandro, Acosta-Quezada, Pablo Geovanny, Riofrío-Cuenca, Tanya Elizabet, Rusch, Douglas B., Bedinger, Patricia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685505/
https://www.ncbi.nlm.nih.gov/pubmed/34938321
http://dx.doi.org/10.3389/fgene.2021.780793
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author Broz, Amanda K.
Miller, Christopher M.
Baek, You Soon
Tovar-Méndez, Alejandro
Acosta-Quezada, Pablo Geovanny
Riofrío-Cuenca, Tanya Elizabet
Rusch, Douglas B.
Bedinger, Patricia A.
author_facet Broz, Amanda K.
Miller, Christopher M.
Baek, You Soon
Tovar-Méndez, Alejandro
Acosta-Quezada, Pablo Geovanny
Riofrío-Cuenca, Tanya Elizabet
Rusch, Douglas B.
Bedinger, Patricia A.
author_sort Broz, Amanda K.
collection PubMed
description The self-incompatibility (SI) system in the Solanaceae is comprised of cytotoxic pistil S-RNases which are countered by S-locus F-box (SLF) resistance factors found in pollen. Under this barrier-resistance architecture, mating system transitions from SI to self-compatibility (SC) typically result from loss-of-function mutations in genes encoding pistil SI factors such as S-RNase. However, the nature of these mutations is often not well characterized. Here we use a combination of S-RNase sequence analysis, transcript profiling, protein expression and reproductive phenotyping to better understand different mechanisms that result in loss of S-RNase function. Our analysis focuses on 12 S-RNase alleles identified in SC species and populations across the tomato clade. In six cases, the reason for gene dysfunction due to mutations is evident. The six other alleles potentially encode functional S-RNase proteins but are typically transcriptionally silenced. We identified three S-RNase alleles which are transcriptionally silenced under some conditions but actively expressed in others. In one case, expression of the S-RNase is associated with SI. In another case, S-RNase expression does not lead to SI, but instead confers a reproductive barrier against pollen tubes from other tomato species. In the third case, expression of S-RNase does not affect self, interspecific or inter-population reproductive barriers. Our results indicate that S-RNase expression is more dynamic than previously thought, and that changes in expression can impact different reproductive barriers within or between natural populations.
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spelling pubmed-86855052021-12-21 S-RNase Alleles Associated With Self-Compatibility in the Tomato Clade: Structure, Origins, and Expression Plasticity Broz, Amanda K. Miller, Christopher M. Baek, You Soon Tovar-Méndez, Alejandro Acosta-Quezada, Pablo Geovanny Riofrío-Cuenca, Tanya Elizabet Rusch, Douglas B. Bedinger, Patricia A. Front Genet Genetics The self-incompatibility (SI) system in the Solanaceae is comprised of cytotoxic pistil S-RNases which are countered by S-locus F-box (SLF) resistance factors found in pollen. Under this barrier-resistance architecture, mating system transitions from SI to self-compatibility (SC) typically result from loss-of-function mutations in genes encoding pistil SI factors such as S-RNase. However, the nature of these mutations is often not well characterized. Here we use a combination of S-RNase sequence analysis, transcript profiling, protein expression and reproductive phenotyping to better understand different mechanisms that result in loss of S-RNase function. Our analysis focuses on 12 S-RNase alleles identified in SC species and populations across the tomato clade. In six cases, the reason for gene dysfunction due to mutations is evident. The six other alleles potentially encode functional S-RNase proteins but are typically transcriptionally silenced. We identified three S-RNase alleles which are transcriptionally silenced under some conditions but actively expressed in others. In one case, expression of the S-RNase is associated with SI. In another case, S-RNase expression does not lead to SI, but instead confers a reproductive barrier against pollen tubes from other tomato species. In the third case, expression of S-RNase does not affect self, interspecific or inter-population reproductive barriers. Our results indicate that S-RNase expression is more dynamic than previously thought, and that changes in expression can impact different reproductive barriers within or between natural populations. Frontiers Media S.A. 2021-12-06 /pmc/articles/PMC8685505/ /pubmed/34938321 http://dx.doi.org/10.3389/fgene.2021.780793 Text en Copyright © 2021 Broz, Miller, Baek, Tovar-Méndez, Acosta-Quezada, Riofrío-Cuenca, Rusch and Bedinger. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Broz, Amanda K.
Miller, Christopher M.
Baek, You Soon
Tovar-Méndez, Alejandro
Acosta-Quezada, Pablo Geovanny
Riofrío-Cuenca, Tanya Elizabet
Rusch, Douglas B.
Bedinger, Patricia A.
S-RNase Alleles Associated With Self-Compatibility in the Tomato Clade: Structure, Origins, and Expression Plasticity
title S-RNase Alleles Associated With Self-Compatibility in the Tomato Clade: Structure, Origins, and Expression Plasticity
title_full S-RNase Alleles Associated With Self-Compatibility in the Tomato Clade: Structure, Origins, and Expression Plasticity
title_fullStr S-RNase Alleles Associated With Self-Compatibility in the Tomato Clade: Structure, Origins, and Expression Plasticity
title_full_unstemmed S-RNase Alleles Associated With Self-Compatibility in the Tomato Clade: Structure, Origins, and Expression Plasticity
title_short S-RNase Alleles Associated With Self-Compatibility in the Tomato Clade: Structure, Origins, and Expression Plasticity
title_sort s-rnase alleles associated with self-compatibility in the tomato clade: structure, origins, and expression plasticity
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685505/
https://www.ncbi.nlm.nih.gov/pubmed/34938321
http://dx.doi.org/10.3389/fgene.2021.780793
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