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Characteristics of SP600125 Induced Tetraploid Cells in Comparison With Diploid and Tetraploid Cells of Fish

In our previous research, SP600125 (Anthrapyrazolone) was used to induce autotetraploid of crucian carp cells (SP4N cells), and tetraploid fry was generated from the SP4N cells by somatic cell nuclear transfer technique. However, it is still unclear about biological characteristics of the SP4N cells...

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Autores principales: Fan, Yunpeng, Zhang, Guangjing, Zhao, Kaiyue, Fu, Wen, Chen, Shujuan, Liu, Jinhui, Liu, Wenbin, Peng, Liangyue, Ren, Li, Liu, Shaojun, Xiao, Yamei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685524/
https://www.ncbi.nlm.nih.gov/pubmed/34938322
http://dx.doi.org/10.3389/fgene.2021.781007
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author Fan, Yunpeng
Zhang, Guangjing
Zhao, Kaiyue
Fu, Wen
Chen, Shujuan
Liu, Jinhui
Liu, Wenbin
Peng, Liangyue
Ren, Li
Liu, Shaojun
Xiao, Yamei
author_facet Fan, Yunpeng
Zhang, Guangjing
Zhao, Kaiyue
Fu, Wen
Chen, Shujuan
Liu, Jinhui
Liu, Wenbin
Peng, Liangyue
Ren, Li
Liu, Shaojun
Xiao, Yamei
author_sort Fan, Yunpeng
collection PubMed
description In our previous research, SP600125 (Anthrapyrazolone) was used to induce autotetraploid of crucian carp cells (SP4N cells), and tetraploid fry was generated from the SP4N cells by somatic cell nuclear transfer technique. However, it is still unclear about biological characteristics of the SP4N cells. In this article, the cytological characteristic and gene expression profiles of the SP4N cells are investigated in comparison with the crucian carp cells (2N cells) and the tetraploid crucian carp cells (CC4N cells). The SP4N cells have tetraploid characteristics in terms of morphology and DNA ploidy levels, and their chromosome behavior is stable during the cell proliferation. The migration ability and the mtDNA copy number of SP4N cells are both lower than those in the CC4N cells and the 2N cells, but there exist giant mitochondria in the SP4N cells. The similar expression trends in the cell cycle regulation genes of the SP4N cells and 2N cells, while the corresponding expression profiles are clearly different between the SP4N cells and the CC4N cells. Moreover, the significant difference genes are associated with energy metabolism pathways among the SP4N cells, 2N cells and CC4N cells. These results can provide deeper understanding of SP600125 induction, as well as finding applications in polyploidization breeding of fish species.
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spelling pubmed-86855242021-12-21 Characteristics of SP600125 Induced Tetraploid Cells in Comparison With Diploid and Tetraploid Cells of Fish Fan, Yunpeng Zhang, Guangjing Zhao, Kaiyue Fu, Wen Chen, Shujuan Liu, Jinhui Liu, Wenbin Peng, Liangyue Ren, Li Liu, Shaojun Xiao, Yamei Front Genet Genetics In our previous research, SP600125 (Anthrapyrazolone) was used to induce autotetraploid of crucian carp cells (SP4N cells), and tetraploid fry was generated from the SP4N cells by somatic cell nuclear transfer technique. However, it is still unclear about biological characteristics of the SP4N cells. In this article, the cytological characteristic and gene expression profiles of the SP4N cells are investigated in comparison with the crucian carp cells (2N cells) and the tetraploid crucian carp cells (CC4N cells). The SP4N cells have tetraploid characteristics in terms of morphology and DNA ploidy levels, and their chromosome behavior is stable during the cell proliferation. The migration ability and the mtDNA copy number of SP4N cells are both lower than those in the CC4N cells and the 2N cells, but there exist giant mitochondria in the SP4N cells. The similar expression trends in the cell cycle regulation genes of the SP4N cells and 2N cells, while the corresponding expression profiles are clearly different between the SP4N cells and the CC4N cells. Moreover, the significant difference genes are associated with energy metabolism pathways among the SP4N cells, 2N cells and CC4N cells. These results can provide deeper understanding of SP600125 induction, as well as finding applications in polyploidization breeding of fish species. Frontiers Media S.A. 2021-12-06 /pmc/articles/PMC8685524/ /pubmed/34938322 http://dx.doi.org/10.3389/fgene.2021.781007 Text en Copyright © 2021 Fan, Zhang, Zhao, Fu, Chen, Liu, Liu, Peng, Ren, Liu and Xiao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Fan, Yunpeng
Zhang, Guangjing
Zhao, Kaiyue
Fu, Wen
Chen, Shujuan
Liu, Jinhui
Liu, Wenbin
Peng, Liangyue
Ren, Li
Liu, Shaojun
Xiao, Yamei
Characteristics of SP600125 Induced Tetraploid Cells in Comparison With Diploid and Tetraploid Cells of Fish
title Characteristics of SP600125 Induced Tetraploid Cells in Comparison With Diploid and Tetraploid Cells of Fish
title_full Characteristics of SP600125 Induced Tetraploid Cells in Comparison With Diploid and Tetraploid Cells of Fish
title_fullStr Characteristics of SP600125 Induced Tetraploid Cells in Comparison With Diploid and Tetraploid Cells of Fish
title_full_unstemmed Characteristics of SP600125 Induced Tetraploid Cells in Comparison With Diploid and Tetraploid Cells of Fish
title_short Characteristics of SP600125 Induced Tetraploid Cells in Comparison With Diploid and Tetraploid Cells of Fish
title_sort characteristics of sp600125 induced tetraploid cells in comparison with diploid and tetraploid cells of fish
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685524/
https://www.ncbi.nlm.nih.gov/pubmed/34938322
http://dx.doi.org/10.3389/fgene.2021.781007
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