Entropy-regularized deconvolution of cellular cryotransmission electron tomograms

Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularize...

Descripción completa

Detalles Bibliográficos
Autores principales: Croxford, Matthew, Elbaum, Michael, Arigovindan, Muthuvel, Kam, Zvi, Agard, David, Villa, Elizabeth, Sedat, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2021
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685678/
https://www.ncbi.nlm.nih.gov/pubmed/34876518
http://dx.doi.org/10.1073/pnas.2108738118
_version_ 1784617878825205760
author Croxford, Matthew
Elbaum, Michael
Arigovindan, Muthuvel
Kam, Zvi
Agard, David
Villa, Elizabeth
Sedat, John
author_facet Croxford, Matthew
Elbaum, Michael
Arigovindan, Muthuvel
Kam, Zvi
Agard, David
Villa, Elizabeth
Sedat, John
author_sort Croxford, Matthew
collection PubMed
description Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA).
format Online
Article
Text
id pubmed-8685678
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher National Academy of Sciences
record_format MEDLINE/PubMed
spelling pubmed-86856782022-01-06 Entropy-regularized deconvolution of cellular cryotransmission electron tomograms Croxford, Matthew Elbaum, Michael Arigovindan, Muthuvel Kam, Zvi Agard, David Villa, Elizabeth Sedat, John Proc Natl Acad Sci U S A Biological Sciences Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA). National Academy of Sciences 2021-12-07 2021-12-14 /pmc/articles/PMC8685678/ /pubmed/34876518 http://dx.doi.org/10.1073/pnas.2108738118 Text en Copyright © 2021 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Croxford, Matthew
Elbaum, Michael
Arigovindan, Muthuvel
Kam, Zvi
Agard, David
Villa, Elizabeth
Sedat, John
Entropy-regularized deconvolution of cellular cryotransmission electron tomograms
title Entropy-regularized deconvolution of cellular cryotransmission electron tomograms
title_full Entropy-regularized deconvolution of cellular cryotransmission electron tomograms
title_fullStr Entropy-regularized deconvolution of cellular cryotransmission electron tomograms
title_full_unstemmed Entropy-regularized deconvolution of cellular cryotransmission electron tomograms
title_short Entropy-regularized deconvolution of cellular cryotransmission electron tomograms
title_sort entropy-regularized deconvolution of cellular cryotransmission electron tomograms
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685678/
https://www.ncbi.nlm.nih.gov/pubmed/34876518
http://dx.doi.org/10.1073/pnas.2108738118
work_keys_str_mv AT croxfordmatthew entropyregularizeddeconvolutionofcellularcryotransmissionelectrontomograms
AT elbaummichael entropyregularizeddeconvolutionofcellularcryotransmissionelectrontomograms
AT arigovindanmuthuvel entropyregularizeddeconvolutionofcellularcryotransmissionelectrontomograms
AT kamzvi entropyregularizeddeconvolutionofcellularcryotransmissionelectrontomograms
AT agarddavid entropyregularizeddeconvolutionofcellularcryotransmissionelectrontomograms
AT villaelizabeth entropyregularizeddeconvolutionofcellularcryotransmissionelectrontomograms
AT sedatjohn entropyregularizeddeconvolutionofcellularcryotransmissionelectrontomograms

Ejemplares similares