A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis

Euglena gracilis is a source of high-value natural products. A major factor affecting consistent production of Euglena biomass is strain stability. Cryopreservation is a leading strategy for cell-line storage that helps ensure process reproducibility. We developed a simple cryopreservation protocol...

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Detalles Bibliográficos
Autores principales: Shah, Mahfuzur R., Morrison, Erin N., Noble, Adam J., Farrow, Scott C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686060/
https://www.ncbi.nlm.nih.gov/pubmed/34977684
http://dx.doi.org/10.1016/j.xpro.2021.101043
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author Shah, Mahfuzur R.
Morrison, Erin N.
Noble, Adam J.
Farrow, Scott C.
author_facet Shah, Mahfuzur R.
Morrison, Erin N.
Noble, Adam J.
Farrow, Scott C.
author_sort Shah, Mahfuzur R.
collection PubMed
description Euglena gracilis is a source of high-value natural products. A major factor affecting consistent production of Euglena biomass is strain stability. Cryopreservation is a leading strategy for cell-line storage that helps ensure process reproducibility. We developed a simple cryopreservation protocol for heterotrophically cultured Euglena that enables the recovery of cells after 1 year with a cell viability of ≅80%. This protocol is suitable for labs interested in the long-term preservation of heterotrophic cultures of Euglena and related species.
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spelling pubmed-86860602021-12-30 A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis Shah, Mahfuzur R. Morrison, Erin N. Noble, Adam J. Farrow, Scott C. STAR Protoc Protocol Euglena gracilis is a source of high-value natural products. A major factor affecting consistent production of Euglena biomass is strain stability. Cryopreservation is a leading strategy for cell-line storage that helps ensure process reproducibility. We developed a simple cryopreservation protocol for heterotrophically cultured Euglena that enables the recovery of cells after 1 year with a cell viability of ≅80%. This protocol is suitable for labs interested in the long-term preservation of heterotrophic cultures of Euglena and related species. Elsevier 2021-12-15 /pmc/articles/PMC8686060/ /pubmed/34977684 http://dx.doi.org/10.1016/j.xpro.2021.101043 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Shah, Mahfuzur R.
Morrison, Erin N.
Noble, Adam J.
Farrow, Scott C.
A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis
title A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis
title_full A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis
title_fullStr A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis
title_full_unstemmed A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis
title_short A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis
title_sort simple and effective cryopreservation protocol for the industrially important and model organism, euglena gracilis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686060/
https://www.ncbi.nlm.nih.gov/pubmed/34977684
http://dx.doi.org/10.1016/j.xpro.2021.101043
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