Protocol to quantify palmitoylation of cysteines in budding yeast

Palmitoylation is a special kind of lipid modification that targets proteins to membranes. This protocol introduces the acyl-biotin exchange (ABE) assay to determine the palmitoylation of protein cysteines in yeast. Palmitoylation is exchanged by biotinylated compounds so that the palmitoyl proteins...

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Detalles Bibliográficos
Autores principales: Lei, Yuqing, Zhu, Jiangli, Li, Huihui, Kong, Eryan, Lu, Kefeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686073/
https://www.ncbi.nlm.nih.gov/pubmed/34977681
http://dx.doi.org/10.1016/j.xpro.2021.101035
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author Lei, Yuqing
Zhu, Jiangli
Li, Huihui
Kong, Eryan
Lu, Kefeng
author_facet Lei, Yuqing
Zhu, Jiangli
Li, Huihui
Kong, Eryan
Lu, Kefeng
author_sort Lei, Yuqing
collection PubMed
description Palmitoylation is a special kind of lipid modification that targets proteins to membranes. This protocol introduces the acyl-biotin exchange (ABE) assay to determine the palmitoylation of protein cysteines in yeast. Palmitoylation is exchanged by biotinylated compounds so that the palmitoyl proteins can be affinity-purified for downstream assay by western blot. This protocol is easy to perform and can be applied to other biological sources with slight modifications. This protocol is limited to the detection of cysteine-based palmitoylation. For complete details on the use and execution of this profile, please refer to Lei et al. (2021).
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spelling pubmed-86860732021-12-30 Protocol to quantify palmitoylation of cysteines in budding yeast Lei, Yuqing Zhu, Jiangli Li, Huihui Kong, Eryan Lu, Kefeng STAR Protoc Protocol Palmitoylation is a special kind of lipid modification that targets proteins to membranes. This protocol introduces the acyl-biotin exchange (ABE) assay to determine the palmitoylation of protein cysteines in yeast. Palmitoylation is exchanged by biotinylated compounds so that the palmitoyl proteins can be affinity-purified for downstream assay by western blot. This protocol is easy to perform and can be applied to other biological sources with slight modifications. This protocol is limited to the detection of cysteine-based palmitoylation. For complete details on the use and execution of this profile, please refer to Lei et al. (2021). Elsevier 2021-12-15 /pmc/articles/PMC8686073/ /pubmed/34977681 http://dx.doi.org/10.1016/j.xpro.2021.101035 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lei, Yuqing
Zhu, Jiangli
Li, Huihui
Kong, Eryan
Lu, Kefeng
Protocol to quantify palmitoylation of cysteines in budding yeast
title Protocol to quantify palmitoylation of cysteines in budding yeast
title_full Protocol to quantify palmitoylation of cysteines in budding yeast
title_fullStr Protocol to quantify palmitoylation of cysteines in budding yeast
title_full_unstemmed Protocol to quantify palmitoylation of cysteines in budding yeast
title_short Protocol to quantify palmitoylation of cysteines in budding yeast
title_sort protocol to quantify palmitoylation of cysteines in budding yeast
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686073/
https://www.ncbi.nlm.nih.gov/pubmed/34977681
http://dx.doi.org/10.1016/j.xpro.2021.101035
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