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Protocol to quantify palmitoylation of cysteines in budding yeast

Palmitoylation is a special kind of lipid modification that targets proteins to membranes. This protocol introduces the acyl-biotin exchange (ABE) assay to determine the palmitoylation of protein cysteines in yeast. Palmitoylation is exchanged by biotinylated compounds so that the palmitoyl proteins...

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Detalles Bibliográficos
Autores principales:	Lei, Yuqing, Zhu, Jiangli, Li, Huihui, Kong, Eryan, Lu, Kefeng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686073/ https://www.ncbi.nlm.nih.gov/pubmed/34977681 http://dx.doi.org/10.1016/j.xpro.2021.101035

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