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Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo

OBJECTIVE: Coronaviruses (CoVs) are natural commensals of bats. Two subgenera, namely Sarbecoviruses and Merbecoviruses have a high zoonotic potential and have been associated with three separate spillover events in the past 2 decades, making surveillance of bat-CoVs crucial for the prevention of th...

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Autores principales: Tan, Cheng-Siang, Noni, Vaenessa, Sathiya Seelan, Jaya Seelan, Denel, Azroie, Anwarali Khan, Faisal Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686085/
https://www.ncbi.nlm.nih.gov/pubmed/34930456
http://dx.doi.org/10.1186/s13104-021-05880-6
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author Tan, Cheng-Siang
Noni, Vaenessa
Sathiya Seelan, Jaya Seelan
Denel, Azroie
Anwarali Khan, Faisal Ali
author_facet Tan, Cheng-Siang
Noni, Vaenessa
Sathiya Seelan, Jaya Seelan
Denel, Azroie
Anwarali Khan, Faisal Ali
author_sort Tan, Cheng-Siang
collection PubMed
description OBJECTIVE: Coronaviruses (CoVs) are natural commensals of bats. Two subgenera, namely Sarbecoviruses and Merbecoviruses have a high zoonotic potential and have been associated with three separate spillover events in the past 2 decades, making surveillance of bat-CoVs crucial for the prevention of the next epidemic. The study was aimed to elucidate the presence of coronavirus in fresh bat guano sampled from Wind Cave Nature Reserve (WCNR) in Sarawak, Malaysian Borneo. Samples collected were placed into viral transport medium, transported on ice within the collection day, and preserved at − 80 °C. Nucleic acid was extracted using the column method and screened using consensus PCR primers targeting the RNA-dependent RNA polymerase (RdRp) gene. Amplicons were sequenced bidirectionally using the Sanger method. Phylogenetic tree with maximum-likelihood bootstrap and Bayesian posterior probability were constructed. RESULTS: CoV-RNA was detected in ten specimens (47.6%, n  = 21). Six alphacoronavirus and four betacoronaviruses were identified. The bat-CoVs can be phylogenetically grouped into four novel clades which are closely related to Decacovirus-1 and Decacovirus-2, Sarbecovirus, and an unclassified CoV. CoVs lineages unique to the Island of Borneo were discovered in Sarawak, Malaysia, with one of them closely related to Sarbecovirus. All of them are distant from currently known human coronaviruses.
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spelling pubmed-86860852021-12-20 Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo Tan, Cheng-Siang Noni, Vaenessa Sathiya Seelan, Jaya Seelan Denel, Azroie Anwarali Khan, Faisal Ali BMC Res Notes Research Note OBJECTIVE: Coronaviruses (CoVs) are natural commensals of bats. Two subgenera, namely Sarbecoviruses and Merbecoviruses have a high zoonotic potential and have been associated with three separate spillover events in the past 2 decades, making surveillance of bat-CoVs crucial for the prevention of the next epidemic. The study was aimed to elucidate the presence of coronavirus in fresh bat guano sampled from Wind Cave Nature Reserve (WCNR) in Sarawak, Malaysian Borneo. Samples collected were placed into viral transport medium, transported on ice within the collection day, and preserved at − 80 °C. Nucleic acid was extracted using the column method and screened using consensus PCR primers targeting the RNA-dependent RNA polymerase (RdRp) gene. Amplicons were sequenced bidirectionally using the Sanger method. Phylogenetic tree with maximum-likelihood bootstrap and Bayesian posterior probability were constructed. RESULTS: CoV-RNA was detected in ten specimens (47.6%, n  = 21). Six alphacoronavirus and four betacoronaviruses were identified. The bat-CoVs can be phylogenetically grouped into four novel clades which are closely related to Decacovirus-1 and Decacovirus-2, Sarbecovirus, and an unclassified CoV. CoVs lineages unique to the Island of Borneo were discovered in Sarawak, Malaysia, with one of them closely related to Sarbecovirus. All of them are distant from currently known human coronaviruses. BioMed Central 2021-12-20 /pmc/articles/PMC8686085/ /pubmed/34930456 http://dx.doi.org/10.1186/s13104-021-05880-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Tan, Cheng-Siang
Noni, Vaenessa
Sathiya Seelan, Jaya Seelan
Denel, Azroie
Anwarali Khan, Faisal Ali
Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
title Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
title_full Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
title_fullStr Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
title_full_unstemmed Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
title_short Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
title_sort ecological surveillance of bat coronaviruses in sarawak, malaysian borneo
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686085/
https://www.ncbi.nlm.nih.gov/pubmed/34930456
http://dx.doi.org/10.1186/s13104-021-05880-6
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