Cargando…

Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366

BACKGROUND: Known as the prerequisite component for the heterosis breeding system, the male sterile line determines the hybrid yield and seed purity. Therefore, a deep understanding of the mechanism and gene network that leads to male sterility is crucial. BS366, a temperature-sensitive genic male s...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yong-jie, Li, Dan, Gong, Jie, Wang, Yong-bo, Chen, Zhao-bo, Pang, Bin-shuang, Chen, Xian-chao, Gao, Jian-gang, Yang, Wei-bing, Zhang, Feng-ting, Tang, Yi-miao, Zhao, Chang-ping, Gao, Shi-qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686610/
https://www.ncbi.nlm.nih.gov/pubmed/34930131
http://dx.doi.org/10.1186/s12864-021-08163-3
_version_ 1784618050715123712
author Liu, Yong-jie
Li, Dan
Gong, Jie
Wang, Yong-bo
Chen, Zhao-bo
Pang, Bin-shuang
Chen, Xian-chao
Gao, Jian-gang
Yang, Wei-bing
Zhang, Feng-ting
Tang, Yi-miao
Zhao, Chang-ping
Gao, Shi-qing
author_facet Liu, Yong-jie
Li, Dan
Gong, Jie
Wang, Yong-bo
Chen, Zhao-bo
Pang, Bin-shuang
Chen, Xian-chao
Gao, Jian-gang
Yang, Wei-bing
Zhang, Feng-ting
Tang, Yi-miao
Zhao, Chang-ping
Gao, Shi-qing
author_sort Liu, Yong-jie
collection PubMed
description BACKGROUND: Known as the prerequisite component for the heterosis breeding system, the male sterile line determines the hybrid yield and seed purity. Therefore, a deep understanding of the mechanism and gene network that leads to male sterility is crucial. BS366, a temperature-sensitive genic male sterile (TGMS) line, is male sterile under cold conditions (12 °C with 12 h of daylight) but fertile under normal temperature (20 °C with 12 h of daylight). RESULTS: During meiosis, BS366 was defective in forming tetrads and dyads due to the abnormal cell plate. During pollen development, unusual vacuolated pollen that could not accumulate starch grains at the binucleate stage was also observed. Transcriptome analysis revealed that genes involved in the meiotic process, such as sister chromatid segregation and microtubule-based movement, were repressed, while genes involved in DNA and histone methylation were induced in BS366 under cold conditions. MethylRAD was used for reduced DNA methylation sequencing of BS366 spikes under both cold and control conditions. The differentially methylated sites (DMSs) located in the gene region were mainly involved in carbohydrate and fatty acid metabolism, lipid metabolism, and transport. Differentially expressed and methylated genes were mainly involved in cell division. CONCLUSIONS: These results indicated that the methylation of genes involved in carbon metabolism or fatty acid metabolism might contribute to male sterility in BS366 spikes, providing novel insight into the molecular mechanism of wheat male sterility. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-08163-3.
format Online
Article
Text
id pubmed-8686610
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-86866102021-12-20 Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366 Liu, Yong-jie Li, Dan Gong, Jie Wang, Yong-bo Chen, Zhao-bo Pang, Bin-shuang Chen, Xian-chao Gao, Jian-gang Yang, Wei-bing Zhang, Feng-ting Tang, Yi-miao Zhao, Chang-ping Gao, Shi-qing BMC Genomics Research BACKGROUND: Known as the prerequisite component for the heterosis breeding system, the male sterile line determines the hybrid yield and seed purity. Therefore, a deep understanding of the mechanism and gene network that leads to male sterility is crucial. BS366, a temperature-sensitive genic male sterile (TGMS) line, is male sterile under cold conditions (12 °C with 12 h of daylight) but fertile under normal temperature (20 °C with 12 h of daylight). RESULTS: During meiosis, BS366 was defective in forming tetrads and dyads due to the abnormal cell plate. During pollen development, unusual vacuolated pollen that could not accumulate starch grains at the binucleate stage was also observed. Transcriptome analysis revealed that genes involved in the meiotic process, such as sister chromatid segregation and microtubule-based movement, were repressed, while genes involved in DNA and histone methylation were induced in BS366 under cold conditions. MethylRAD was used for reduced DNA methylation sequencing of BS366 spikes under both cold and control conditions. The differentially methylated sites (DMSs) located in the gene region were mainly involved in carbohydrate and fatty acid metabolism, lipid metabolism, and transport. Differentially expressed and methylated genes were mainly involved in cell division. CONCLUSIONS: These results indicated that the methylation of genes involved in carbon metabolism or fatty acid metabolism might contribute to male sterility in BS366 spikes, providing novel insight into the molecular mechanism of wheat male sterility. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-08163-3. BioMed Central 2021-12-20 /pmc/articles/PMC8686610/ /pubmed/34930131 http://dx.doi.org/10.1186/s12864-021-08163-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liu, Yong-jie
Li, Dan
Gong, Jie
Wang, Yong-bo
Chen, Zhao-bo
Pang, Bin-shuang
Chen, Xian-chao
Gao, Jian-gang
Yang, Wei-bing
Zhang, Feng-ting
Tang, Yi-miao
Zhao, Chang-ping
Gao, Shi-qing
Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366
title Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366
title_full Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366
title_fullStr Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366
title_full_unstemmed Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366
title_short Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366
title_sort comparative transcriptome and dna methylation analysis in temperature-sensitive genic male sterile wheat bs366
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686610/
https://www.ncbi.nlm.nih.gov/pubmed/34930131
http://dx.doi.org/10.1186/s12864-021-08163-3
work_keys_str_mv AT liuyongjie comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT lidan comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT gongjie comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT wangyongbo comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT chenzhaobo comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT pangbinshuang comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT chenxianchao comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT gaojiangang comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT yangweibing comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT zhangfengting comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT tangyimiao comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT zhaochangping comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366
AT gaoshiqing comparativetranscriptomeanddnamethylationanalysisintemperaturesensitivegenicmalesterilewheatbs366