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Phenotypic Expression of Oral Fibroblasts Derived from Oral Submucous Fibrosis: An Assay through Cell Culture
OBJECTIVE: The present study aimed to establish cell lines of fibroblast from human OSF tissues and their response to varying concentrations of arecoline. The various morphological forms of fibroblasts were identified to establish phenotypic change. MATERIALS AND METHOD: Fibroblast cell lines were o...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686880/ https://www.ncbi.nlm.nih.gov/pubmed/35017962 http://dx.doi.org/10.4103/jpbs.jpbs_408_21 |
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author | Banerjee, Abhishek Mampilly, Mathew O. Kamath, V. V. Athreya, Vijayaraghavan Kotrashetti, Vijayalakshmi Srivastava, Kumar Chandan Shrivastava, Deepti |
author_facet | Banerjee, Abhishek Mampilly, Mathew O. Kamath, V. V. Athreya, Vijayaraghavan Kotrashetti, Vijayalakshmi Srivastava, Kumar Chandan Shrivastava, Deepti |
author_sort | Banerjee, Abhishek |
collection | PubMed |
description | OBJECTIVE: The present study aimed to establish cell lines of fibroblast from human OSF tissues and their response to varying concentrations of arecoline. The various morphological forms of fibroblasts were identified to establish phenotypic change. MATERIALS AND METHOD: Fibroblast cell lines were obtained from control samples as well as from OSF cases. The cell lines were treated with 50/100/150/300/500 ug/ml of arecoline and morphology were determined. RESULTS: Three morphological forms were detected; F1 spindle, F2 epitheloid and the F3 stellate. The F3 to F1 ratio was higher in OSF. Arecoline at 50ug/ml was stimulatory and at 150ug/ml cytotoxic to the cell lines. CONCLUSION: Arecoline seems to enhance proliferation of the fibroblast at lower concentrations but cytotoxic at higher levels. This is probably due to the generation of new cell lines and response of the arecoline receptors indicating phenotypic change. |
format | Online Article Text |
id | pubmed-8686880 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-86868802022-01-10 Phenotypic Expression of Oral Fibroblasts Derived from Oral Submucous Fibrosis: An Assay through Cell Culture Banerjee, Abhishek Mampilly, Mathew O. Kamath, V. V. Athreya, Vijayaraghavan Kotrashetti, Vijayalakshmi Srivastava, Kumar Chandan Shrivastava, Deepti J Pharm Bioallied Sci Original Article OBJECTIVE: The present study aimed to establish cell lines of fibroblast from human OSF tissues and their response to varying concentrations of arecoline. The various morphological forms of fibroblasts were identified to establish phenotypic change. MATERIALS AND METHOD: Fibroblast cell lines were obtained from control samples as well as from OSF cases. The cell lines were treated with 50/100/150/300/500 ug/ml of arecoline and morphology were determined. RESULTS: Three morphological forms were detected; F1 spindle, F2 epitheloid and the F3 stellate. The F3 to F1 ratio was higher in OSF. Arecoline at 50ug/ml was stimulatory and at 150ug/ml cytotoxic to the cell lines. CONCLUSION: Arecoline seems to enhance proliferation of the fibroblast at lower concentrations but cytotoxic at higher levels. This is probably due to the generation of new cell lines and response of the arecoline receptors indicating phenotypic change. Wolters Kluwer - Medknow 2021-11 2021-11-10 /pmc/articles/PMC8686880/ /pubmed/35017962 http://dx.doi.org/10.4103/jpbs.jpbs_408_21 Text en Copyright: © 2021 Journal of Pharmacy and Bioallied Sciences https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Banerjee, Abhishek Mampilly, Mathew O. Kamath, V. V. Athreya, Vijayaraghavan Kotrashetti, Vijayalakshmi Srivastava, Kumar Chandan Shrivastava, Deepti Phenotypic Expression of Oral Fibroblasts Derived from Oral Submucous Fibrosis: An Assay through Cell Culture |
title | Phenotypic Expression of Oral Fibroblasts Derived from Oral Submucous Fibrosis: An Assay through Cell Culture |
title_full | Phenotypic Expression of Oral Fibroblasts Derived from Oral Submucous Fibrosis: An Assay through Cell Culture |
title_fullStr | Phenotypic Expression of Oral Fibroblasts Derived from Oral Submucous Fibrosis: An Assay through Cell Culture |
title_full_unstemmed | Phenotypic Expression of Oral Fibroblasts Derived from Oral Submucous Fibrosis: An Assay through Cell Culture |
title_short | Phenotypic Expression of Oral Fibroblasts Derived from Oral Submucous Fibrosis: An Assay through Cell Culture |
title_sort | phenotypic expression of oral fibroblasts derived from oral submucous fibrosis: an assay through cell culture |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686880/ https://www.ncbi.nlm.nih.gov/pubmed/35017962 http://dx.doi.org/10.4103/jpbs.jpbs_408_21 |
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