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miRNA-144-5p/ITGA3 Suppressed the Tumor-Promoting Behaviors of Thyroid Cancer Cells by Downregulating ITGA3

OBJECTIVE: To ascertain the mechanism of miRNA-144-5p and ITGA3 in thyroid cancer (TC). METHODS: From The Cancer Genome Atlas (TCGA), RNA expression profiles were obtained for the expression analysis of miRNAs and mRNAs in TC. qRT-PCR and western blot were utilized to measure the expression of miRNA...

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Detalles Bibliográficos
Autores principales: Zhang, Jizong, Zhong, Yan, Sang, Yiming, Ren, Guanghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8687768/
https://www.ncbi.nlm.nih.gov/pubmed/34938358
http://dx.doi.org/10.1155/2021/9181941
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author Zhang, Jizong
Zhong, Yan
Sang, Yiming
Ren, Guanghui
author_facet Zhang, Jizong
Zhong, Yan
Sang, Yiming
Ren, Guanghui
author_sort Zhang, Jizong
collection PubMed
description OBJECTIVE: To ascertain the mechanism of miRNA-144-5p and ITGA3 in thyroid cancer (TC). METHODS: From The Cancer Genome Atlas (TCGA), RNA expression profiles were obtained for the expression analysis of miRNAs and mRNAs in TC. qRT-PCR and western blot were utilized to measure the expression of miRNA-144-5p and ITGA3 at RNA and protein levels, respectively. The association between miRNA-144-5p and ITGA3 was validated by the dual-luciferase assay. CCK-8, scratch healing, transwell, and flow cytometry assays were employed to evaluate tumor-related cell behaviors. RESULTS: Low-expressed miRNA-144-5p and high-expressed ITGA3 were found in TC cells relative to normal cells. miRNA-144-5p expression was positively associated with suppressive effects on proliferative, invasive, and migratory ability of TC cells while negatively associated with cell apoptosis. miRNA-144-5p inhibited ITGA3 expression in TC, and its overexpression remarkably reversed the tumor-promoting effects of overexpressed ITGA3 on the biological functions of TC. CONCLUSION: It is verified in our study that cell growth of TC is inhibited by the miRNA-144-5p/ITGA3 axis, which represents an underlying target for TC. This research proposed a new insight into the strategy of TC treatment.
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spelling pubmed-86877682021-12-21 miRNA-144-5p/ITGA3 Suppressed the Tumor-Promoting Behaviors of Thyroid Cancer Cells by Downregulating ITGA3 Zhang, Jizong Zhong, Yan Sang, Yiming Ren, Guanghui Comput Math Methods Med Research Article OBJECTIVE: To ascertain the mechanism of miRNA-144-5p and ITGA3 in thyroid cancer (TC). METHODS: From The Cancer Genome Atlas (TCGA), RNA expression profiles were obtained for the expression analysis of miRNAs and mRNAs in TC. qRT-PCR and western blot were utilized to measure the expression of miRNA-144-5p and ITGA3 at RNA and protein levels, respectively. The association between miRNA-144-5p and ITGA3 was validated by the dual-luciferase assay. CCK-8, scratch healing, transwell, and flow cytometry assays were employed to evaluate tumor-related cell behaviors. RESULTS: Low-expressed miRNA-144-5p and high-expressed ITGA3 were found in TC cells relative to normal cells. miRNA-144-5p expression was positively associated with suppressive effects on proliferative, invasive, and migratory ability of TC cells while negatively associated with cell apoptosis. miRNA-144-5p inhibited ITGA3 expression in TC, and its overexpression remarkably reversed the tumor-promoting effects of overexpressed ITGA3 on the biological functions of TC. CONCLUSION: It is verified in our study that cell growth of TC is inhibited by the miRNA-144-5p/ITGA3 axis, which represents an underlying target for TC. This research proposed a new insight into the strategy of TC treatment. Hindawi 2021-12-13 /pmc/articles/PMC8687768/ /pubmed/34938358 http://dx.doi.org/10.1155/2021/9181941 Text en Copyright © 2021 Jizong Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Jizong
Zhong, Yan
Sang, Yiming
Ren, Guanghui
miRNA-144-5p/ITGA3 Suppressed the Tumor-Promoting Behaviors of Thyroid Cancer Cells by Downregulating ITGA3
title miRNA-144-5p/ITGA3 Suppressed the Tumor-Promoting Behaviors of Thyroid Cancer Cells by Downregulating ITGA3
title_full miRNA-144-5p/ITGA3 Suppressed the Tumor-Promoting Behaviors of Thyroid Cancer Cells by Downregulating ITGA3
title_fullStr miRNA-144-5p/ITGA3 Suppressed the Tumor-Promoting Behaviors of Thyroid Cancer Cells by Downregulating ITGA3
title_full_unstemmed miRNA-144-5p/ITGA3 Suppressed the Tumor-Promoting Behaviors of Thyroid Cancer Cells by Downregulating ITGA3
title_short miRNA-144-5p/ITGA3 Suppressed the Tumor-Promoting Behaviors of Thyroid Cancer Cells by Downregulating ITGA3
title_sort mirna-144-5p/itga3 suppressed the tumor-promoting behaviors of thyroid cancer cells by downregulating itga3
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8687768/
https://www.ncbi.nlm.nih.gov/pubmed/34938358
http://dx.doi.org/10.1155/2021/9181941
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