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miR-10c Facilitates White Spot Syndrome Virus Infection by Targeting Toll3 in Litopenaeus vannemei

Toll-like receptors (TLRs) are canonical cell membrane receptors functioning to recognize pathogens and transduce signals to activate immune responses. It has been known that Toll3 in Pacific white shrimp Litopenaeus vannamei (LvToll3) plays a critical role in antiviral immunity by inducing the tran...

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Autores principales: Zuo, Hongliang, Liu, Xinxin, Luo, Mengting, Yang, Linwei, Zhu, Zhiming, Weng, Shaoping, He, Jianguo, Xu, Xiaopeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8688535/
https://www.ncbi.nlm.nih.gov/pubmed/34950131
http://dx.doi.org/10.3389/fimmu.2021.733730
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author Zuo, Hongliang
Liu, Xinxin
Luo, Mengting
Yang, Linwei
Zhu, Zhiming
Weng, Shaoping
He, Jianguo
Xu, Xiaopeng
author_facet Zuo, Hongliang
Liu, Xinxin
Luo, Mengting
Yang, Linwei
Zhu, Zhiming
Weng, Shaoping
He, Jianguo
Xu, Xiaopeng
author_sort Zuo, Hongliang
collection PubMed
description Toll-like receptors (TLRs) are canonical cell membrane receptors functioning to recognize pathogens and transduce signals to activate immune responses. It has been known that Toll3 in Pacific white shrimp Litopenaeus vannamei (LvToll3) plays a critical role in antiviral immunity by inducing the transcription of interferon regulatory factor (IRF), which mediates a signaling axis that is similar to the interferon system of vertebrates. However, the regulatory mechanism of the Toll3-IRF signaling is still unclear. In this study, a novel microRNA (miRNA) of miR-10 family, temporarily named as miR-10c, was identified from L. vannamei. miR-10c may play a nonnegligible regulatory role in shrimp immune responses since it was constitutively expressed in all detected tissues and transcriptionally induced by immune stimulation. Functional analysis validated that miR-10c could target LvToll3 to inhibit its expression, through which miR-10c blocked the nuclear translocation of IRF and facilitated white spot syndrome virus (WSSV) infection. To our knowledge, the present study revealed the first report of a Toll targeted by miRNA in crustaceans and provided a solid evidence base for supporting the role of LvToll3 in antiviral defense by activating IRF signaling in L. vannamei. Identification of the miR-10c/Toll3/IRF regulatory axis in shrimp provides new insights into the participation of miRNA in the regulation of immune responses and contributes to in-depth understanding of the mechanisms of Toll-induced immune responses in L. vannamei.
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spelling pubmed-86885352021-12-22 miR-10c Facilitates White Spot Syndrome Virus Infection by Targeting Toll3 in Litopenaeus vannemei Zuo, Hongliang Liu, Xinxin Luo, Mengting Yang, Linwei Zhu, Zhiming Weng, Shaoping He, Jianguo Xu, Xiaopeng Front Immunol Immunology Toll-like receptors (TLRs) are canonical cell membrane receptors functioning to recognize pathogens and transduce signals to activate immune responses. It has been known that Toll3 in Pacific white shrimp Litopenaeus vannamei (LvToll3) plays a critical role in antiviral immunity by inducing the transcription of interferon regulatory factor (IRF), which mediates a signaling axis that is similar to the interferon system of vertebrates. However, the regulatory mechanism of the Toll3-IRF signaling is still unclear. In this study, a novel microRNA (miRNA) of miR-10 family, temporarily named as miR-10c, was identified from L. vannamei. miR-10c may play a nonnegligible regulatory role in shrimp immune responses since it was constitutively expressed in all detected tissues and transcriptionally induced by immune stimulation. Functional analysis validated that miR-10c could target LvToll3 to inhibit its expression, through which miR-10c blocked the nuclear translocation of IRF and facilitated white spot syndrome virus (WSSV) infection. To our knowledge, the present study revealed the first report of a Toll targeted by miRNA in crustaceans and provided a solid evidence base for supporting the role of LvToll3 in antiviral defense by activating IRF signaling in L. vannamei. Identification of the miR-10c/Toll3/IRF regulatory axis in shrimp provides new insights into the participation of miRNA in the regulation of immune responses and contributes to in-depth understanding of the mechanisms of Toll-induced immune responses in L. vannamei. Frontiers Media S.A. 2021-12-07 /pmc/articles/PMC8688535/ /pubmed/34950131 http://dx.doi.org/10.3389/fimmu.2021.733730 Text en Copyright © 2021 Zuo, Liu, Luo, Yang, Zhu, Weng, He and Xu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Zuo, Hongliang
Liu, Xinxin
Luo, Mengting
Yang, Linwei
Zhu, Zhiming
Weng, Shaoping
He, Jianguo
Xu, Xiaopeng
miR-10c Facilitates White Spot Syndrome Virus Infection by Targeting Toll3 in Litopenaeus vannemei
title miR-10c Facilitates White Spot Syndrome Virus Infection by Targeting Toll3 in Litopenaeus vannemei
title_full miR-10c Facilitates White Spot Syndrome Virus Infection by Targeting Toll3 in Litopenaeus vannemei
title_fullStr miR-10c Facilitates White Spot Syndrome Virus Infection by Targeting Toll3 in Litopenaeus vannemei
title_full_unstemmed miR-10c Facilitates White Spot Syndrome Virus Infection by Targeting Toll3 in Litopenaeus vannemei
title_short miR-10c Facilitates White Spot Syndrome Virus Infection by Targeting Toll3 in Litopenaeus vannemei
title_sort mir-10c facilitates white spot syndrome virus infection by targeting toll3 in litopenaeus vannemei
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8688535/
https://www.ncbi.nlm.nih.gov/pubmed/34950131
http://dx.doi.org/10.3389/fimmu.2021.733730
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