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Quantitative proteomic dataset of the moss Physcomitrium patens PSEP3 KO and OE mutant lines

Small open reading frames (<100 codons) that are located on long noncoding RNAs (lncRNAs) can encode functional microproteins. These microproteins are shown to play important roles in different cellular processes, such as cell proliferation, development and disease response [1], [2], [3], [4], [5...

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Autores principales: Mamaeva, Anna, Knyazev, Andrey, Glushkevich, Anna, Fesenko, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8688553/
https://www.ncbi.nlm.nih.gov/pubmed/34977300
http://dx.doi.org/10.1016/j.dib.2021.107715
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author Mamaeva, Anna
Knyazev, Andrey
Glushkevich, Anna
Fesenko, Igor
author_facet Mamaeva, Anna
Knyazev, Andrey
Glushkevich, Anna
Fesenko, Igor
author_sort Mamaeva, Anna
collection PubMed
description Small open reading frames (<100 codons) that are located on long noncoding RNAs (lncRNAs) can encode functional microproteins. These microproteins are shown to play important roles in different cellular processes, such as cell proliferation, development and disease response [1], [2], [3], [4], [5], [6]. However, there are only a few known lncRNA-encoded functional microproteins in plants. One such microprotein that was named PSEP3, was identified in the moss Physcomitrium patens by mass-spectrometry analysis. 57-aa PSEP3 contains Low Complexity Region (LCR) enriched with proline. We have previously shown that PSEP3 is translated in protonemata and gametophores of P. patens, and its knockout (KO line) or overexpression (OE line) affects protonemata growth [7]. We performed a quantitative proteomic analysis of the mutant lines with PSEP3 knockout and overexpression. 7-days old protonemata of wild type (WT line) and both mutant lines (KO and OE) were collected and used for iTRAQ-based proteomic experiments. LC-MS/MS data were processed using PEAKS Studio v.8 software with protein identification based on a Phytozome protein database. More analysis of PSEP3 effects on plant growth can be obtained in the paper published in Nucleic Acid Research [8].
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spelling pubmed-86885532021-12-30 Quantitative proteomic dataset of the moss Physcomitrium patens PSEP3 KO and OE mutant lines Mamaeva, Anna Knyazev, Andrey Glushkevich, Anna Fesenko, Igor Data Brief Data Article Small open reading frames (<100 codons) that are located on long noncoding RNAs (lncRNAs) can encode functional microproteins. These microproteins are shown to play important roles in different cellular processes, such as cell proliferation, development and disease response [1], [2], [3], [4], [5], [6]. However, there are only a few known lncRNA-encoded functional microproteins in plants. One such microprotein that was named PSEP3, was identified in the moss Physcomitrium patens by mass-spectrometry analysis. 57-aa PSEP3 contains Low Complexity Region (LCR) enriched with proline. We have previously shown that PSEP3 is translated in protonemata and gametophores of P. patens, and its knockout (KO line) or overexpression (OE line) affects protonemata growth [7]. We performed a quantitative proteomic analysis of the mutant lines with PSEP3 knockout and overexpression. 7-days old protonemata of wild type (WT line) and both mutant lines (KO and OE) were collected and used for iTRAQ-based proteomic experiments. LC-MS/MS data were processed using PEAKS Studio v.8 software with protein identification based on a Phytozome protein database. More analysis of PSEP3 effects on plant growth can be obtained in the paper published in Nucleic Acid Research [8]. Elsevier 2021-12-16 /pmc/articles/PMC8688553/ /pubmed/34977300 http://dx.doi.org/10.1016/j.dib.2021.107715 Text en © 2021 The Author(s). Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Data Article
Mamaeva, Anna
Knyazev, Andrey
Glushkevich, Anna
Fesenko, Igor
Quantitative proteomic dataset of the moss Physcomitrium patens PSEP3 KO and OE mutant lines
title Quantitative proteomic dataset of the moss Physcomitrium patens PSEP3 KO and OE mutant lines
title_full Quantitative proteomic dataset of the moss Physcomitrium patens PSEP3 KO and OE mutant lines
title_fullStr Quantitative proteomic dataset of the moss Physcomitrium patens PSEP3 KO and OE mutant lines
title_full_unstemmed Quantitative proteomic dataset of the moss Physcomitrium patens PSEP3 KO and OE mutant lines
title_short Quantitative proteomic dataset of the moss Physcomitrium patens PSEP3 KO and OE mutant lines
title_sort quantitative proteomic dataset of the moss physcomitrium patens psep3 ko and oe mutant lines
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8688553/
https://www.ncbi.nlm.nih.gov/pubmed/34977300
http://dx.doi.org/10.1016/j.dib.2021.107715
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