Cargando…
A Label-Free Immunosensor Based on Gold Nanoparticles/Thionine for Sensitive Detection of PAT Protein in Genetically Modified Crops
Genetically modified (GM) crops containing phosphinothricin acetyltransferase (PAT) protein has been widely planted worldwide. The development of a rapid method for detecting PAT protein is of great importance to food supervision. In this study, a simple label-free electrochemical immunosensor for t...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8688707/ https://www.ncbi.nlm.nih.gov/pubmed/34950635 http://dx.doi.org/10.3389/fchem.2021.770584 |
_version_ | 1784618400381665280 |
---|---|
author | Yang, Qianwen Wang, Yu Liu, Xiaofeng Liu, Hua Bao, Huifang Wang, Jinbin Zeng, Haijuan |
author_facet | Yang, Qianwen Wang, Yu Liu, Xiaofeng Liu, Hua Bao, Huifang Wang, Jinbin Zeng, Haijuan |
author_sort | Yang, Qianwen |
collection | PubMed |
description | Genetically modified (GM) crops containing phosphinothricin acetyltransferase (PAT) protein has been widely planted worldwide. The development of a rapid method for detecting PAT protein is of great importance to food supervision. In this study, a simple label-free electrochemical immunosensor for the ultrasensitive detection of PAT protein was constructed using thionine (Thi)/gold nanoparticles (AuNPs) as signal amplification molecules and electrochemically active substances. Under optimum conditions, the limits of detection of the sensor for soybean A2704-12 and maize BT-176 were 0.02% and 0.03%, respectively. The sensor could detect crops containing PAT protein and had no cross-reaction with other proteins. After storage at 4°C for 33 days, the sensor still retained 82.5% of the original signal, with a relative standard deviation (RSD) of 0.92%. The recoveries of the sensor for soybean A2704-12 and maize BT-176 were 85%–108% and 98%–113%, respectively. The developed PAT-target immunosensor with high sensitivity, specificity, and satisfactory reproducibility and accuracy will be a useful tool in the trace screening of GM crops. Moreover, this design concept can be extended to other proteins by simply changing the antibody. |
format | Online Article Text |
id | pubmed-8688707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86887072021-12-22 A Label-Free Immunosensor Based on Gold Nanoparticles/Thionine for Sensitive Detection of PAT Protein in Genetically Modified Crops Yang, Qianwen Wang, Yu Liu, Xiaofeng Liu, Hua Bao, Huifang Wang, Jinbin Zeng, Haijuan Front Chem Chemistry Genetically modified (GM) crops containing phosphinothricin acetyltransferase (PAT) protein has been widely planted worldwide. The development of a rapid method for detecting PAT protein is of great importance to food supervision. In this study, a simple label-free electrochemical immunosensor for the ultrasensitive detection of PAT protein was constructed using thionine (Thi)/gold nanoparticles (AuNPs) as signal amplification molecules and electrochemically active substances. Under optimum conditions, the limits of detection of the sensor for soybean A2704-12 and maize BT-176 were 0.02% and 0.03%, respectively. The sensor could detect crops containing PAT protein and had no cross-reaction with other proteins. After storage at 4°C for 33 days, the sensor still retained 82.5% of the original signal, with a relative standard deviation (RSD) of 0.92%. The recoveries of the sensor for soybean A2704-12 and maize BT-176 were 85%–108% and 98%–113%, respectively. The developed PAT-target immunosensor with high sensitivity, specificity, and satisfactory reproducibility and accuracy will be a useful tool in the trace screening of GM crops. Moreover, this design concept can be extended to other proteins by simply changing the antibody. Frontiers Media S.A. 2021-12-07 /pmc/articles/PMC8688707/ /pubmed/34950635 http://dx.doi.org/10.3389/fchem.2021.770584 Text en Copyright © 2021 Yang, Wang, Liu, Liu, Bao, Wang and Zeng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Yang, Qianwen Wang, Yu Liu, Xiaofeng Liu, Hua Bao, Huifang Wang, Jinbin Zeng, Haijuan A Label-Free Immunosensor Based on Gold Nanoparticles/Thionine for Sensitive Detection of PAT Protein in Genetically Modified Crops |
title | A Label-Free Immunosensor Based on Gold Nanoparticles/Thionine for Sensitive Detection of PAT Protein in Genetically Modified Crops |
title_full | A Label-Free Immunosensor Based on Gold Nanoparticles/Thionine for Sensitive Detection of PAT Protein in Genetically Modified Crops |
title_fullStr | A Label-Free Immunosensor Based on Gold Nanoparticles/Thionine for Sensitive Detection of PAT Protein in Genetically Modified Crops |
title_full_unstemmed | A Label-Free Immunosensor Based on Gold Nanoparticles/Thionine for Sensitive Detection of PAT Protein in Genetically Modified Crops |
title_short | A Label-Free Immunosensor Based on Gold Nanoparticles/Thionine for Sensitive Detection of PAT Protein in Genetically Modified Crops |
title_sort | label-free immunosensor based on gold nanoparticles/thionine for sensitive detection of pat protein in genetically modified crops |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8688707/ https://www.ncbi.nlm.nih.gov/pubmed/34950635 http://dx.doi.org/10.3389/fchem.2021.770584 |
work_keys_str_mv | AT yangqianwen alabelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT wangyu alabelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT liuxiaofeng alabelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT liuhua alabelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT baohuifang alabelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT wangjinbin alabelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT zenghaijuan alabelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT yangqianwen labelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT wangyu labelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT liuxiaofeng labelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT liuhua labelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT baohuifang labelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT wangjinbin labelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops AT zenghaijuan labelfreeimmunosensorbasedongoldnanoparticlesthionineforsensitivedetectionofpatproteiningeneticallymodifiedcrops |