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Laminar Organization of the Entorhinal Cortex in Macaque Monkeys Based on Cell-Type-Specific Markers and Connectivity

The entorhinal cortex (EC) is a major gateway between the hippocampus and telencephalic structures, and plays a critical role in memory and navigation. Through the use of various molecular markers and genetic tools, neuron types constituting EC are well studied in rodents, and their layer-dependent...

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Autores principales: Ohara, Shinya, Yoshino, Rintaro, Kimura, Kei, Kawamura, Taichi, Tanabe, Soshi, Zheng, Andi, Nakamura, Shinya, Inoue, Ken-ichi, Takada, Masahiko, Tsutsui, Ken-Ichiro, Witter, Menno P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8688913/
https://www.ncbi.nlm.nih.gov/pubmed/34949991
http://dx.doi.org/10.3389/fncir.2021.790116
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author Ohara, Shinya
Yoshino, Rintaro
Kimura, Kei
Kawamura, Taichi
Tanabe, Soshi
Zheng, Andi
Nakamura, Shinya
Inoue, Ken-ichi
Takada, Masahiko
Tsutsui, Ken-Ichiro
Witter, Menno P.
author_facet Ohara, Shinya
Yoshino, Rintaro
Kimura, Kei
Kawamura, Taichi
Tanabe, Soshi
Zheng, Andi
Nakamura, Shinya
Inoue, Ken-ichi
Takada, Masahiko
Tsutsui, Ken-Ichiro
Witter, Menno P.
author_sort Ohara, Shinya
collection PubMed
description The entorhinal cortex (EC) is a major gateway between the hippocampus and telencephalic structures, and plays a critical role in memory and navigation. Through the use of various molecular markers and genetic tools, neuron types constituting EC are well studied in rodents, and their layer-dependent distributions, connections, and functions have also been characterized. In primates, however, such cell-type-specific understandings are lagging. To bridge the gap between rodents and primates, here we provide the first cell-type-based global map of EC in macaque monkeys. The laminar organization of the monkey EC was systematically examined and compared with that of the rodent EC by using immunohistochemistry for molecular markers which have been well characterized in the rodent EC: reelin, calbindin, and Purkinje cell protein 4 (PCP4). We further employed retrograde neuron labeling from the nucleus accumbens and amygdala to identify the EC output layer. This cell-type-based approach enabled us to apply the latest laminar definition of rodent EC to monkeys. Based on the similarity of the laminar organization, the monkey EC can be divided into two subdivisions: rostral and caudal EC. These subdivisions likely correspond to the lateral and medial EC in rodents, respectively. In addition, we found an overall absence of a clear laminar arrangement of layer V neurons in the rostral EC, unlike rodents. The cell-type-based architectural map provided in this study will accelerate the application of genetic tools in monkeys for better understanding of the role of EC in memory and navigation.
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spelling pubmed-86889132021-12-22 Laminar Organization of the Entorhinal Cortex in Macaque Monkeys Based on Cell-Type-Specific Markers and Connectivity Ohara, Shinya Yoshino, Rintaro Kimura, Kei Kawamura, Taichi Tanabe, Soshi Zheng, Andi Nakamura, Shinya Inoue, Ken-ichi Takada, Masahiko Tsutsui, Ken-Ichiro Witter, Menno P. Front Neural Circuits Neural Circuits The entorhinal cortex (EC) is a major gateway between the hippocampus and telencephalic structures, and plays a critical role in memory and navigation. Through the use of various molecular markers and genetic tools, neuron types constituting EC are well studied in rodents, and their layer-dependent distributions, connections, and functions have also been characterized. In primates, however, such cell-type-specific understandings are lagging. To bridge the gap between rodents and primates, here we provide the first cell-type-based global map of EC in macaque monkeys. The laminar organization of the monkey EC was systematically examined and compared with that of the rodent EC by using immunohistochemistry for molecular markers which have been well characterized in the rodent EC: reelin, calbindin, and Purkinje cell protein 4 (PCP4). We further employed retrograde neuron labeling from the nucleus accumbens and amygdala to identify the EC output layer. This cell-type-based approach enabled us to apply the latest laminar definition of rodent EC to monkeys. Based on the similarity of the laminar organization, the monkey EC can be divided into two subdivisions: rostral and caudal EC. These subdivisions likely correspond to the lateral and medial EC in rodents, respectively. In addition, we found an overall absence of a clear laminar arrangement of layer V neurons in the rostral EC, unlike rodents. The cell-type-based architectural map provided in this study will accelerate the application of genetic tools in monkeys for better understanding of the role of EC in memory and navigation. Frontiers Media S.A. 2021-12-07 /pmc/articles/PMC8688913/ /pubmed/34949991 http://dx.doi.org/10.3389/fncir.2021.790116 Text en Copyright © 2021 Ohara, Yoshino, Kimura, Kawamura, Tanabe, Zheng, Nakamura, Inoue, Takada, Tsutsui and Witter. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neural Circuits
Ohara, Shinya
Yoshino, Rintaro
Kimura, Kei
Kawamura, Taichi
Tanabe, Soshi
Zheng, Andi
Nakamura, Shinya
Inoue, Ken-ichi
Takada, Masahiko
Tsutsui, Ken-Ichiro
Witter, Menno P.
Laminar Organization of the Entorhinal Cortex in Macaque Monkeys Based on Cell-Type-Specific Markers and Connectivity
title Laminar Organization of the Entorhinal Cortex in Macaque Monkeys Based on Cell-Type-Specific Markers and Connectivity
title_full Laminar Organization of the Entorhinal Cortex in Macaque Monkeys Based on Cell-Type-Specific Markers and Connectivity
title_fullStr Laminar Organization of the Entorhinal Cortex in Macaque Monkeys Based on Cell-Type-Specific Markers and Connectivity
title_full_unstemmed Laminar Organization of the Entorhinal Cortex in Macaque Monkeys Based on Cell-Type-Specific Markers and Connectivity
title_short Laminar Organization of the Entorhinal Cortex in Macaque Monkeys Based on Cell-Type-Specific Markers and Connectivity
title_sort laminar organization of the entorhinal cortex in macaque monkeys based on cell-type-specific markers and connectivity
topic Neural Circuits
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8688913/
https://www.ncbi.nlm.nih.gov/pubmed/34949991
http://dx.doi.org/10.3389/fncir.2021.790116
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