Cargando…

Characterization of Enterovirus Associated m6A RNA Methylation in Children With Neurological Symptoms: A Prospective Cohort Study

Little is known about the particular changes of N6-methyladenosine (m6A) RNA methylation in enterovirus (EV) infection among children with neurologic symptoms. Here, we determined the characterization of EV associated m6A RNA methylation in this population. A prospective cohort study was conducted f...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Danping, Song, Yongling, Hu, Dandan, Li, Suyun, Liu, Guangming, Li, Peiqing, Yang, Sida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689127/
https://www.ncbi.nlm.nih.gov/pubmed/34949987
http://dx.doi.org/10.3389/fnins.2021.791544
_version_ 1784618485080391680
author Zhu, Danping
Song, Yongling
Hu, Dandan
Li, Suyun
Liu, Guangming
Li, Peiqing
Yang, Sida
author_facet Zhu, Danping
Song, Yongling
Hu, Dandan
Li, Suyun
Liu, Guangming
Li, Peiqing
Yang, Sida
author_sort Zhu, Danping
collection PubMed
description Little is known about the particular changes of N6-methyladenosine (m6A) RNA methylation in enterovirus (EV) infection among children with neurologic symptoms. Here, we determined the characterization of EV associated m6A RNA methylation in this population. A prospective cohort study was conducted from 2018/2 to 2019/12 at the Guangzhou Women and Children’s Medical Center. We included EV infected children with and without neurological symptoms. High-throughput m(6)A-RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-seq analysis were used to evaluate the m6A RNA methylation and transcript expression of cerebrospinal fluid samples. The functional annotation and pathways of differentially methylated m6A genes with synchronously differential expression were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Seven patients were enrolled in the control group, and 13 cases were in the neurological symptoms (NS) group. A total of 3472 differentially expressed genes and 957 m6A modified genes were identified. A conjoint analysis of MeRIP-seq and RNA-seq data found 1064 genes with significant changes in both the m6A modifications and mRNA levels. The different m6A RNA methylation was increased in the transcriptome’s CDS regions but decreased in both the 3′UTRs and stop codon among the NS group. Functional annotation like the “oxidative phosphorylation” gene pathway, “Parkinson’s disease” and GO terms like “respiratory electron transport chain,” “cellular metabolic process,” and “oxidation-reduction process” was enriched in symptomatic patients. Our study elucidated the changes of RNA m6A methylation patterns and related cellular functions and signaling pathways in EV patients with neurologic symptoms.
format Online
Article
Text
id pubmed-8689127
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86891272021-12-22 Characterization of Enterovirus Associated m6A RNA Methylation in Children With Neurological Symptoms: A Prospective Cohort Study Zhu, Danping Song, Yongling Hu, Dandan Li, Suyun Liu, Guangming Li, Peiqing Yang, Sida Front Neurosci Neuroscience Little is known about the particular changes of N6-methyladenosine (m6A) RNA methylation in enterovirus (EV) infection among children with neurologic symptoms. Here, we determined the characterization of EV associated m6A RNA methylation in this population. A prospective cohort study was conducted from 2018/2 to 2019/12 at the Guangzhou Women and Children’s Medical Center. We included EV infected children with and without neurological symptoms. High-throughput m(6)A-RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-seq analysis were used to evaluate the m6A RNA methylation and transcript expression of cerebrospinal fluid samples. The functional annotation and pathways of differentially methylated m6A genes with synchronously differential expression were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Seven patients were enrolled in the control group, and 13 cases were in the neurological symptoms (NS) group. A total of 3472 differentially expressed genes and 957 m6A modified genes were identified. A conjoint analysis of MeRIP-seq and RNA-seq data found 1064 genes with significant changes in both the m6A modifications and mRNA levels. The different m6A RNA methylation was increased in the transcriptome’s CDS regions but decreased in both the 3′UTRs and stop codon among the NS group. Functional annotation like the “oxidative phosphorylation” gene pathway, “Parkinson’s disease” and GO terms like “respiratory electron transport chain,” “cellular metabolic process,” and “oxidation-reduction process” was enriched in symptomatic patients. Our study elucidated the changes of RNA m6A methylation patterns and related cellular functions and signaling pathways in EV patients with neurologic symptoms. Frontiers Media S.A. 2021-12-07 /pmc/articles/PMC8689127/ /pubmed/34949987 http://dx.doi.org/10.3389/fnins.2021.791544 Text en Copyright © 2021 Zhu, Song, Hu, Li, Liu, Li and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Zhu, Danping
Song, Yongling
Hu, Dandan
Li, Suyun
Liu, Guangming
Li, Peiqing
Yang, Sida
Characterization of Enterovirus Associated m6A RNA Methylation in Children With Neurological Symptoms: A Prospective Cohort Study
title Characterization of Enterovirus Associated m6A RNA Methylation in Children With Neurological Symptoms: A Prospective Cohort Study
title_full Characterization of Enterovirus Associated m6A RNA Methylation in Children With Neurological Symptoms: A Prospective Cohort Study
title_fullStr Characterization of Enterovirus Associated m6A RNA Methylation in Children With Neurological Symptoms: A Prospective Cohort Study
title_full_unstemmed Characterization of Enterovirus Associated m6A RNA Methylation in Children With Neurological Symptoms: A Prospective Cohort Study
title_short Characterization of Enterovirus Associated m6A RNA Methylation in Children With Neurological Symptoms: A Prospective Cohort Study
title_sort characterization of enterovirus associated m6a rna methylation in children with neurological symptoms: a prospective cohort study
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689127/
https://www.ncbi.nlm.nih.gov/pubmed/34949987
http://dx.doi.org/10.3389/fnins.2021.791544
work_keys_str_mv AT zhudanping characterizationofenterovirusassociatedm6arnamethylationinchildrenwithneurologicalsymptomsaprospectivecohortstudy
AT songyongling characterizationofenterovirusassociatedm6arnamethylationinchildrenwithneurologicalsymptomsaprospectivecohortstudy
AT hudandan characterizationofenterovirusassociatedm6arnamethylationinchildrenwithneurologicalsymptomsaprospectivecohortstudy
AT lisuyun characterizationofenterovirusassociatedm6arnamethylationinchildrenwithneurologicalsymptomsaprospectivecohortstudy
AT liuguangming characterizationofenterovirusassociatedm6arnamethylationinchildrenwithneurologicalsymptomsaprospectivecohortstudy
AT lipeiqing characterizationofenterovirusassociatedm6arnamethylationinchildrenwithneurologicalsymptomsaprospectivecohortstudy
AT yangsida characterizationofenterovirusassociatedm6arnamethylationinchildrenwithneurologicalsymptomsaprospectivecohortstudy