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Cryo-EM analysis of Ebola virus nucleocapsid-like assembly

This protocol describes the reconstitution of the filamentous Ebola virus nucleocapsid-like assembly in vitro. This is followed by solving the cryo-EM structure using helical reconstruction, and flexible fitting of the existing model into the 5.8 Å cryo-EM map. The protocol can be applied to other f...

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Detalles Bibliográficos
Autores principales:	Wang, Yan, Binning, Jennifer M., Pintilie, Grigore D., Chiu, Wah, Amarasinghe, Gaya K., Leung, Daisy W., Su, Zhaoming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689349/ https://www.ncbi.nlm.nih.gov/pubmed/34977676 http://dx.doi.org/10.1016/j.xpro.2021.101030

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